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D Bosch

Publications and source records attributed to D Bosch.

59 records · Page 4Linked to original sources
PubMed1985

Analysis of the regulatory region of the ssb gene of Escherichia coli.

The regulation of the ssb gene of E. coli has been studied. We reported earlier that the SOS box of the neighbouring uvrA gene also controls the transcription of the ssb gene. Detailed analysis of the upstream region of ssb by S1 mapping reveals the existence of three in vivo functional promoters of which the most upstream one (PI) is inducible by DNA damage. Measurement of galactokinase synthesis using galK fusion plasmids indicates that the uninduced level of transcription from the PI promoter is low. Ssb multicopy plasmids lacking the PI promoter still complement the UV sensitivity of an Ssb mutant. The role of the three promoters in the regulation of the level of Ssb protein in the cell, is discussed.

Chromosome Mapping↗
PubMedDate not supplied

A common regulatory region shared by divergently transcribed genes of the Escherichia coli SOS system.

The Escherichia coli single-stranded DNA binding protein (SSB) is implicated in DNA replication, recombination and repair. On the chromosome, the ssb gene is located adjacent to the excision repair gene uvrA, but the two genes are transcribed in opposite directions. uvrA has been shown to be part of the E. coli SOS system by introducing Mud(Ap, lac) insertions distal to the regulatory region of the gene in the chromosome. Recent investigations suggest that SSB is also involved in the SOS response. However, because the SSB protein is essential to the cell, the inducibility of the ssb gene cannot be investigated by the insertion method. Therefore, we used plasmids harbouring the regulatory region of ssb fused to the galK structural gene, while leaving an intact ssb gene in the chromosome. We show here that expression of the ssb gene is dependent on two promoters of which one is damage inducible. Evidence is presented that the divergently transcribed ssb and uvrA genes are controlled by a common LexA binding site.

Bacterial Proteins↗
PubMedDate not supplied

Safety, efficacy, and cost savings in an outpatient intravenous antibiotic program.

We analyzed the costs of an outpatient intravenous (IV) treatment program using a broad-spectrum, third-generation cephalosporin. Fifty-six patients were treated for various infections in a hospital-based outpatient IV antibiotic program. The mean length of outpatient treatment was 7.4 days (range, 2 to 24 days). Infections in 53 patients resolved successfully, with only 3 patients experiencing recurrence. Side effect were minimal, with no significant toxicities; one case of oral candidiasis and one case of rash occurred. Collectively, the 56 patients were treated for 286 days in the hospital and 414 days in an outpatient program. Costs were totaled on a weekly basis and divided by seven to arrive at a daily charge; the antibiotic most frequently prescribed was used as the representative regimen. The mean cost for each hospital day was $417 compared with $155 per outpatient day, a savings of $262 on a daily basis. We conclude that outpatient IV antibiotic therapy is safe and effective for a variety of infections in a wide range of patients and can contribute to substantial financial savings.

Adolescent↗