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Biomedical subjects

D Bout

Publications and source records attributed to D Bout.

At least 37 records · Page 2Linked to original sources
PubMed1977

Circulating immune complexes in schistosomiasis.

Circulating immune complexes (CIC) were investigated by the [125I]Clq binding test, the complement fixation test (CFT) and optical density measurement after redissolving 3% polyethylene glycol precipitates of serum from patients infected by Schistosoma mansoni. A highly significant correlation was obtained among these three techniques. More than 60% of the patients demonstrated significantly higher values than control individuals. The level of CIC was found to be higher in the mild than in the hepatosplenic form of the disease. Parasite antigen, IgG, IgM and IgE were characterized in these CIC. In experimental schistosomiasis in mice, maximum levels of CIC, evaluated by the CFT, were observed between the 40th and the 70th day of infection.

Animals↗
PubMed1976

Detection and characterization of circulating immune complexes in rheumatoid arthritis.

Circulating immune complexes (IC) were detected and quantified in serum of patients with rheumatoid arthritis (RA) by three methods: radiolabeled Clq binding test, anticomplementary activity and optical density of 3% PEG precipitated sera. Rheumatoid factor (RF) is characterized in PEG precipitates by the Waaler-Rose reaction. The relationship between IC, complement and RF levels are analyzed.

Antigen-Antibody Complex↗
PubMed1976

Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis.

A specific antigen of Paracoccidioides brasiliensis was isolated from a metabolic extract of the fungus. The extraction was made by specific adsorption to and subsequent elution from a column containing a cross linked polymer to which the antibodies of a monospecific rabbit serum had been covalently attached. The purity of the final product was demonstrated by immunodiffusion analysis of the eluate using immune serum produced in a sensitized rabbit. The purified antigen was shown to have cationic electrophoretic mobility and alkaline phosphatase activity.

Alkaline Phosphatase↗
PubMed1975

Quantitative determination of specific IgE antibodies to Echinococcus granulosus and IgE levels in sera from patients with hydatid disease.

High serum IgE levels were found in eighty-nine patients with hydatid disease. Specific IgE antibodies to E. granulosus hydatid fluid or to a pure E. granulosus antigen were isolated using immunoabsorbents and quantified by a radioimmunoassay. The procedure made it possible to quantify, for the first time, the concentration of IgE antibodies in units per millilitre. Significant levels of IgE antibodies were demonstrated in twenty-three out of thirty-nine patients, by the use of standardized hydatid fluid antigens. The amount of IgE antibodies to E. granulosus was not related to serological findings. However, a highly significant correlation was obtained between levels of total serum IgE and IgE antibodies. A pure antigenic fraction prepared from hydatid fluid and known to be specific for E. granulosus was shown to be the most potent antigen reactive with IgE antibodies. The relationship of IgE antibodies to the viability of the hydatid cyst and to the serum IgE level is discussed.

Antibody Specificity↗
PubMedDate not supplied

Further studies on the circulating M antigen in human and experimental Schistosoma mansoni infections.

Circulating M antigen, previously described in urine from patients infected by Schistosoma mansoni, was shown in serum from infected patients, using human anti-M immune serum with immunodiffusion and immunoelectrophoretic analyses. This antigen was also shown to be present in the serum and urine from infected hamsters, in the urine from infected rabbits and in the serum from infected mice. Generally, it appeared on day 20 after infection. M antigen was specific for the genus Schistosoma and for the immature and adult worm stage. Its electrophoretic migration was cathodic. The molecular weight of urinary M antigen was around 45,000 daltons. The M antigen was thermostable, soluble in trichloroacetic acid, and contained no lipid component. It was hydrolyzed by protease, ribonuclease, amylase or neuraminidase, but was destroyed by sodium metaperiodate. All these properties betoken the polysaccharidic nature of M antigen.

Animals↗
PubMedDate not supplied

Characterization of allergens in Schistosoma mansoni, Fasciola hepatica and Echinococcus granulosus.

Various antigens interacting specifically with IgE antibodies from infected humans were identified in S. mansoni, F. hepatica and E. granulosus soluble extracts by means of radioimmunoelectrophoresis. Two allergens common to adult worm and cercariae were identified in S. mansoni extracts. They proved to be distinct from genus--or species--specific antigens previously identified in the parasite. In adult F. hepatica extract a species-specific antigen and another lipoprotein were shown to interact with IgE antibodies of patients with fasciolasis. Two major allergens were also found in whole fluid of sheep E. granulosus cysts. One of them corresponded to Echinococcus genus specific antigen. These results are discussed according to immunological findings in parasitic infections.

Allergens↗