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D Bout

Publications and source records attributed to D Bout.

At least 73 records · Page 4Linked to original sources
PubMed1980

Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen.

Circulating M antigen, specific for genus Schistosoma, was previously described in serum, urine, patients' milk, and in serum and urine of animals infected by S. mansoni. The M antigen was thermostable and soluble in trichloroacetic acid. It was not hydrolyzed by protease, ribonuclease, amylase, or neuraminidase but destroyed by sodium metaperiodate. In the present study, we have purified the M ag by using trichloroacetic acid solubility, DEAE Sephadex, and immunoadsorption. The M ag showed a neutral electric charge, a m.w. heterogeneity, and was only stained by periodic acid-Schiff. The composition study revealed M ag was a glycoprotein with a polysaccharide moiety (63% of the molecules) particularly rich in galactose, fucose, glucosamine, and mannose, and with a high molecular ratio of serine and threonine. The presence of O-glycosidic linkage allowed M ag to be considered as a mucin or a mucus glycoprotein-like component. It was localized in the cell wall of the gut of adult worms.

Amino Acids↗
PubMed1980

Evaluation of the enzyme-linked immunosorbent assay (ELISA) and other serological tests for the diagnosis of toxoplasmosis.

The enzyme-linked immunosorbent assay (ELISA) was evaluated in human toxoplasmosis in three laboratories using their own procedures. The same batch of serum samples was investigated in the three laboratories. ELISA results were compared by statistical analysis both with one another and with those of the dye test (DT), immunofluorescence (IF), complement fixation test (CFT), and indirect haemagglutination (IHA).Highly significant correlations were obtained between the three laboratories with ELISA using two different antigens and enzyme conjugates. The correlations between ELISA and the other serological tests showed the following sequence: CFT>IF>IHA>DT. Highly significant correlations were obtained between ELISA using anti-gamma-chain and anti-total immunoglobulin conjugates. The agreement in discrimination between sera with low and high antibody levels was good for all the different ELISA techniques but discrimination between positive and negative sera depended rather on the ELISA procedure used.

Enzyme-Linked Immunosorbent Assay↗
PubMed1980

Homocytotropic antibody responses during murine schistosomiasis. A follow-up study of both total immunoglobulins and Schistosoma mansoni specific antibodies.

Mice infected with Schistosoma mansoni develop a high increase of homocytotropic antibodies, thought to be involved in protection. IgE and IgG1 rise differently during the course of infection. Total serum IgE levels rise between days 20 and 30 and the maximum is observed around day 50. IgG1 levels remain almost unchanged until day 50 after which they increase dramatically, reaching a peak at day 100. S. mansoni specific IgE antibodies measured by passive cutaneous anaphylaxis parallel to variations of total IgE. On the contrary, S. mansoni specific IgG1 antibodies, also measured by passive cutaneous anaphylaxis, do not parallel total IgG1; they reach a peak between days 30 and 40 and remain high until day 100.

Animals↗
PubMed1979

Automation of enzyme-linked immunosorbent assay (ELISA).

A prototype of automatized enzyme-linked immunosorbent assay (ELISA) in tubes is described, using a commercially available basic material, easily modified. Nine hundred samples could be completely studied in a day by only one person. The different steps of the automatized ELISA were systematically studied to obtain the best performance. Its application is described in toxoplasmosis serodiagnosis.

Autoanalysis↗
PubMed1979

Purification of a surface-specific soluble antigen from Listeria monocytogenes.

A complex antigenic preparation obtained from Listeria monocytogenes serovariant 4b by freeze-pressing, centrifugation, and gel filtration treatment was studied by crossed immunoelectrophoresis, with the aim of preparing an antigenic fraction that could be used to investigate the serological response to listeric infection. Of 17 immunoprecipitates revealed in the soluble extract, one of three major antigens (designated antigen 2) was shown to be a strong antigen in humans or rabbits infected with L. monocytogenes serovariant 4b. A monospecific antiantigen 2 serum was obtained and used to prepare a serologically homogeneous antigen by immunoadsorption. Antigen 2, most probably located on the bacterial surface, is common to all serovariants of L. monocytogenes and to Listeria grayi and is not shared by the main bacterial species known to have common antigens with L. monocytogenes.

Antibodies, Bacterial↗
PubMed1979

Immunodiagnosis of hydatidosis using monospecific immune serum anti Ag5.

Microdouble diffusion technique using whole hydatid antigen and monospecific antiserum against Echinococcus genus-specific antigen 5 was applied to the diagnosis of human hydatid disease. The use of this simple and economical method may be extended to the specific diagnosis of parasitic diseases.

Animals↗
PubMed1978

Eosinophil-dependent cytotoxicity in rat schistosomiasis. Involvement of IgG2a antibody and role of mast cells.

Using Schistoma mansoni schistosomula collected in vitro and sensitized with 42-day-infected rat serum, normal rat peritoneal cells enriched in eosinophils were proven to subsequently adhere to and kill the schistosomula within 24 to 48 h. The cell-dependent, heat-stable antibody in infected rat serum reached a peak between 30 and 42 days after infection. Inhibition experiments with aggregated immunoglobulins indicated the role of IgG2a antibody in the adherence of eosinophils to sensitized schistosomula. The immune absorption technique showed that IgG2a antibody was involved in the mechanism of cytotoxicity of effector to target cells, whereas the role of IgE antibody could be excluded. Ultrastructural studies revealed the constant presence of eosinophils and mast cells in contact with schistosomula. The use of purified cell populations showed that the cytotoxic effect of the original cell population was significantly decreased after depletion of mast cells and partially restored after addition of mast cells. These observations, together with those concerning the role of IgE immune complexes in macrophage cytotoxicity, suggest the possible participation of anaphylactic antibodies in immunity to schistosomes in the rat.

Animals↗
PubMed1978

Inhibition enzyme immunoassay, application to human apolipoprotein B.

Inhibition enzyme immunoassay was applied to human apolipoprotein B (apo-B) from plasma. The technical conditions of the assay were determined. The detection limits of the assay were 200 ng to 10 microgram/ml. Correlation coefficients obtained between enzymoassay and rocket immunoelectrophoresis on one hand and radial immunodiffusion on the other were respectively 0.84 and 0.80. The inhibition enzymoassay provides a specific and highly sensitive method for the quantitation of apo-B.

Antibodies↗
PubMed1978

Enzyme immunoassay for human apolipoprotein B, the major protein moiety in low-density- and very-low-density lipoproteins.

We used enzyme immunoassay to measure apolipoprotein B concentration in human plasma. Pure lipoprotein B was isolated from serum samples of fasting normolipidemic subjects by sequential preparative ultracentrifugation and coated to a polystyrene tube surface by adsorption. Human serum samples and rabbit antiserum to human apolipoprotein B were incubated with the solid-phase lipoprotein B. Soluble antigen competed with solid-phase antigen for binding to antibodies. After washing, peroxidase-labeled sheep antibodies against rabbit immunoglobulins were added, and after further washing the bound label was assayed. This provided a direct measurement of the soluble antigen. The best technical conditions for the assay were determined. The minimum detectable concentration was 1 microgram per assay. The enzyme immunoassay yielded values that compare favorably with those obtained by radial immunodiffusion (r = 0.84) and by rocket immunoelectrophoresis (r = 0.80). The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, ane non-use of radioisotopes.

Apolipoproteins↗
PubMed1977

Mother-child relationship in human schistosomiasis mansoni. I. Parasitic antigens and antibodies in milk.

Immunoglobulins, anti-Schistosoma mansoni antibodies, complement components and schistosome antigens were investigated in milk from mothers infected with S. mansoni. No significant differences of immunoglobulins or complement component levels were observed between infected and control mothers. Anti-S. mansoni antibodies were detected in the milk of 8 out of 25 infected mothers. A significant relationship was observed between serum and "4", were demonstrated in milk from infected patients by the double diffusion micromethod. The function of these immunologically active substances transmitted by milk from mother to child is discussed.

Antibodies↗
PubMed1977

Enzyme immunoassay for the serodiagnosis of parasitic infections.

A review of some applications of the Enzyme-Linked Immunosorbent Assay (ELISA) for the serodiagnosis of parasitic infections is presented. Examples are given of applications with trichinellosis, hydatidosis, schistosomiasis, toxoplasmosis, african trypanosomiasis and amoebiasis. ELISA is believed to be a valuable addition to existing serological tools, especially due to its sensitivity and ease of performance.

Echinococcosis↗
PubMed1977

Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E.

A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.

Antigens, Fungal↗