Biomedical subjects

D C Cardinal

Publications and source records attributed to D C Cardinal.

9 recordsLinked to original sources

PubMed1981

Measuring platelet and leucocyte aggregation/adhesion responses in very small volumes of whole blood.

A technique is described by which leucocyte and platelet aggregation may be measured in 100 microliter (or less) of citrated whole blood. The blood is first diluted in a balanced salt solution and aggregation responses measured with an electronic aggregometer. Leucocytes may be aggregated with substances such as the chemotactic peptide f-met-leu-phe, and platelets with the conventional agents such as ADP or collagen. The response of the leucocytes to f-met-leu-phe is very sensitive to the inhibitory activity of mepacrine. The aggregation of platelets, but not leucocytes, is blocked by low concentrations of indomethacin. The technique is useful for drug screening, diagnostic purposes, ad investigating platelet and leucocyte pharmacology and physiology.

Animals↗

PubMed1980

The electronic aggregometer: a novel device for assessing platelet behavior in blood.

A novel device for measuring platelet aggregation has been devised. The technique, which depends upon changes in electrical impedance caused by platelet accretion onto electrodes, is suitable for measurements of aggregation in either platelet-rich plasma (PRP) or blood. In PRP both turbidometric and electronic techniques give very similar responses to collagen, thrombin, prostaglandin endoperoxides, ADP, and arachidonic acid, although the electronic aggregometer gives no "shape-change" information and was somewhat more sensitive. In whole blood all these stimuli gave similar dose-related responses to those seen in PRP. The new technique is very suitable for investigating platelet pharmacology, since the inhibitors of aggregation, such as indomethacin and prostacyclin, can be conveniently quantitated in blood by using this technique.

Adenosine Diphosphate↗

PubMed1980

A photometric device for measuring blood vessel diameter in the microcirculation.

A simple photometric device has been developed for measuring changes in blood vessel diameter in the microcirculation in conjunction with a monochrome television microscope. The device consists of a photocell mounted at the end of a small conical hood, which is attached to the screen of the television monitor. The photocell responds to changes in light emitted from the screen during dilatation or constriction of a blood vessel, and a continuous record of these changes is obtained by displaying the output from the photocell on a pen recorder. By using one or more photocells, mounted in separate hoods, the diameter of a number of vessels can be recorded simultaneously. The device has been used to record changes in the diameter of precapillary arterioles and postcapillary venules in the hamster cheek pouch microcirculation. The device has the advantage over image-shearing techniques of being automatic, and it is also considerably less expensive than a number of automated electro-optical or scanning systems.

Animals↗

PubMed1979