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D C Jain

Publications and source records attributed to D C Jain.

At least 73 records · Page 4Linked to original sources
PubMed1990

Sero-epidemiology of measles--a three years prospective study in a rural population of Rajasthan.

A community based prospective study on measles was undertaken during 1986 to 1988 in Ramgarh village of Alwar district (Rajasthan) to elucidate epidemiological features of measles. The initial population of the village was 5258 with 2018 children (0-14 years) which rose to total population of 5923 with 2200 children in 1988. During the entire period of study, all the children (0-14 years) were covered regularly through monthly domicilliary visits by trained paramedical personnel under direct supervision of Medical Officers. A total of 208 measles cases were detected which gave an overall incidence rate of 31.5 per 1000 children (0-14 years) per year. Incidence rate was highest in children of 2-5 years of age group and lowest (4.6 per 1000) in 10-14 years of age group. The maximum number (86.5 per cent) of cases occurred during the first six months of year. The Kolmogrov-Smirnov statistical method validated the seasonal character of the disease (Vn = 5.36, p less than 0.01). A significant (P less than 0.005) rise in seropositivity with increase in age was observed in children (6-36 months) who had no previous history of measles and measles immunization during their life time. A higher rate of sero-conversion was observed in children vaccinated after 10-months of age than those before. No significant relationship of seroconversion following vaccination could be seen with age of vaccination (p greater than 0.50), sex and nutritional status (p greater than 0.10).

Child↗
PubMed1989

Male sexual dysfunction after stroke.

Male sexual functions namely libido and potency were evaluated in fourteen right handed stroke patients. Half of the studied patients had reduced libido while reduced potency was found in 86% patients. Right hemispheric stroke patients had significantly higher reduction in libido as compared to left hemispheric stroke patients. Libido may have some specific cortical or limbic representation in right cerebral hemisphere.

Cerebral Infarction↗
PubMed1989

Detection of Wuchereria bancrofti antigen in serum and finger prick blood samples by enzyme immunoassay: field evaluation.

We have recently reported that a monoclonal antibody-based enzyme immunoassay for detection of filarial antigen in human serum is sensitive and specific for active infection with Wuchereria bancrofti. The present studies were undertaken to assess the feasibility of testing whole blood collected by finger prick in this assay. A preliminary study was performed to compare antigen test results obtained with whole blood, blood dried on filter paper, and serum. Results obtained with anticoagulated whole blood specimens agrees with serum results in 94 of 97 cases. All whole blood and serum specimens from 28 people with positive microfilaria smears were positive in the test. Filter paper blood specimens were less satisfactory because of decreased sensitivity and specificity. A population-based survey of 1009 persons was conducted in a village near Calicut, India, to evaluate the use of finger prick blood specimens for filarial antigen detection in field studies. Thirty-eight of 39 microfilaria carriers had positive antigen tests. In addition, 10.7% of amicrofilaremic endemic controls had positive tests. Additional studies are needed to test the hypothesis that filarial antigenemia in endemic controls indicates the presence of subclinical infection.

Adolescent↗
PubMed1989

Epidemiology of bancroftian filariasis in a semi-urban community of Kerala State.

A community-based longitudinal study was initiated in 1983 in a semi-urban area, endemic for bancroftian filariasis to determine the incidence and prevalence, natural history and clinical pattern of filarial infection and also to study the impact of control measures on it. The base-line filariometric indices observed during the initial clinico-parasitological survey and entomological observations made during the first 12 months of this study are highlighted in this paper. 5.5 per cent of the examined population had microfilaraemia with average infestation of 8.1 per 20 cmm. The microfilaraemia found to have significant (P less than 0.001) relationship with age, but not with sex (P greater than 0.05). The filarial disease which had the prevalence of 3.6 per cent showed a significant (P less than 0.001) relationship to both age and sex. The youngest microfilaria (mf) carrier was of 2 years and with filarial swelling was of 6 years age. A significant (P less than 0.001) relationship of educational status and family size was observed with disease while it was non-significant (P greater than 0.05) with microfilaraemia. With the increase in per capita income of the individuals, a significant decrease in number of persons with microfilaraemia (P less than 0.01) as well as with disease (P less than 0.05) was noted. Culex quinquifasciatus, the vector for this infection was prevalent throughout the period of observation with highest density in September. Vector infection and infectivity rates ranged from 0 to 4.4 per cent and 0 to 2.2 per cent respectively.

Adolescent↗
PubMed1989

Epidemiology of brugian filariasis in a rural community of Kerala State.

The baseline clinico-parasitological parameters collected during the initial survey and the entomological observations made during the first year as a part of a longitudinal study undertaken in a brugian endemic rural community are presented in this paper. Out of the total (11.604) examined population, 8.1 per cent had microfilaraemia and 7.6 per cent had filarial disease. A highly significant (P less than 0.001) relationship of age was observed with microfilaraemia and the disease. The filarial disease rate showed no significant difference between two sexes while the microfilaria (Mf) rate was significantly (P less than 0.001) higher in males than females. A significant (P less than 0.001) relationship of microfilaraemia as well as of filarial disease was found with the income and the educational status of the individual. The mf and the disease rates did not differ by the family size except those families with 1-3 members. However, the relationship of family size was found to be significant (P less than 0.001) with disease and non-significant (P greater than 0.05) with microfilaraemia. The vectors were prevalent throughout the year but the infection was mostly confined to Mansonia annulifera.

Adolescent↗
PubMed1988

Persistence of parasite antigenemia following diethylcarbamazine therapy of bancroftian filariasis.

This study was designed to reexamine the efficacy of diethylcarbamazine for bancroftian filariasis with special reference to changes in serum parasite antigen levels and antifilarial antibody titers after treatment. Patients with asymptomatic microfilaremia were treated with 6 mg/kg diethylcarbamazine daily for 12 days. Microfilaria counts fell dramatically after treatment, as expected. IgG antibody titers to adult and microfilarial antigens of B. malayi were increased 1 month after treatment in most patients. Titers fell slowly to or below pretreatment levels, but remained positive during subsequent months. Parasite antigen levels, measured by monoclonal antibody-based enzyme immunoassay, decreased to 72%, 58%, 53%, and 48% of pretreatment values 1, 3, 6, and 12 months after diethylcarbamazine treatment, respectively. Parasite antigen levels decreased similarly in subjects with and without residual microfilaremia after treatment. These results suggest that diethylcarbamazine has only partial macrofilaricidal activity against W. bancrofti with this dosage schedule. The sustained, impressive reductions in microfilaria counts after treatment despite significant persistence of parasite antigenemia may be explained by sublethal effects of the drug on adult worms. We believe that parasite antigen detection represents a valuable new approach for monitoring the efficacy of antifilarial drug therapy which we hope will lead to improved use of existing drugs and aid in the evaluation of new drugs for filariasis.

Animals↗
PubMed1987

A monoclonal antibody-based enzyme immunoassay for detecting parasite antigenemia in bancroftian filariasis.

We evaluated a monoclonal antibody-based enzyme immunoassay for detecting soluble parasite antigen in sera collected in an area in South India endemic for Wuchereria bancrofti. Filarial antigen was detected in sera from 56 of 57 microfilaremic patients, 9 of 64 aminofilaremic patients with clinical filariasis, and 11 of 70 endemic controls. Antigen was not detected in sera from patients from nonendemic areas who had a variety of other filarial and nonfilarial helminth infections. Parasite antigen titers were significantly correlated with microfilarial counts in night blood smears (r = .64, P less than .01). Negative antigen tests in patients with clinical filariasis may be explained in part by antibody-mediated clearance of circulating antigen. Antibodies to circulating W. bancrofti antigen were detected in 41 of 55 antigen-negative sera from patients with clinical filariasis. Despite this limitation, detecting parasite antigen by enzyme immunoassay provides significant advantages over previously available methods for diagnosing active W. bancrofti infection.

Antibodies↗
PubMed1987

Evaluation of specific humoral immune responses in human filariasis.

Using the antigens prepared from adult worms of B. malayi and D. immitis, a total of 37 malayan and 150 bancroftian serum samples were analysed for the presence of filarial specific antibodies by counterimmunoelectrophoresis and indirect enzyme linked immunosorbent assay. It was observed that microfilaraemic cases do show good specific humoral immune responses against homologous as well as heterologous antigen. Of the two antigens used, B. malayi adult antigen was found to be superior to D. immitis antigen in the detection of filarial antibodies.

Animals↗
PubMed1986

Detection of circulating parasite antigen in bancroftian filariasis by counterimmunoelectrophoresis.

We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble filarial antigen in sera collected in a Wuchereria bancrofti-endemic area in South India. Filarial antigen was detected in 38 of 38 sera from microfilaremic patients, 3 of 48 sera from amicrofilaremic patients with lymphatic pathology, and 3 of 5 sera from former microfilaria carriers with negative blood examinations 6 months or more after diethylcarbamazine therapy. One of 32 endemic control sera, 0 of 35 nonendemic sera, and 0 of 20 B. malayi sera were positive. Antigenemia was equally detectable in sera collected at night or during the day (when microfilariae are absent from the blood). Parasite antigen was also detected in the urine of patients with positive serum tests. Antibodies to circulating filarial antigen (also detected by CIEP) were absent in all but 2 antigen-positive sera but present in 22 of 45 antigen-negative sera from clinical filariasis patients and in 9 of 31 antigen-negative sera from endemic controls. Parasite antigen detection by CIEP appears to be a sensitive, specific, and practical diagnostic test for active W. bancrofti infection.

Antibodies↗