PubMed Health⌕ Search

Biomedical subjects

D C James

Publications and source records attributed to D C James.

18 recordsLinked to original sources
PubMed1990

The use of enzyme immunoassay (EIA) and radiobinding assay to investigate the cross-reactivity of Klebsiella antigens and HLA B27 in ankylosing spondylitis patients and healthy controls.

The binding of rabbit anti-Klebsiella antibodies to tissue-typed lymphocytes obtained from 30 ankylosing spondylitis (AS) patients and 54 healthy subjects has been measured by an enzyme immunoassay method. HLA B27 positive lymphocytes obtained from either AS patients (t = 3.60; p less than 0.001) or healthy subjects (t = 3.77; p less than 0.001) were found to bind Klebsiella antibodies to a significantly greater extent than non-B27 lymphocytes obtained from healthy controls. Absorption studies demonstrated that HLA B27 positive lymphocytes absorbed out significantly more anti-Klebsiella antibodies than HLA B27-negative lymphocytes (t = 6.76; p less than 0.005). These results are compatible with cross-reactivity or molecular mimicry between HLA B27 and epitopes on some Gram-negative bacteria such as Klebsiella.

Antigens, Bacterial↗
PubMed1979

Inferior caval venography in the assessment of renal carcinoma.

Inferior caval venography by a non-catheter technique was carried out in 30 cases of renal cell carcinoma. Occlusion of the renal vein was shown in 15 of these and occlusion of the inferior vena cava in a further three. Deformity of the vena cava by adjacent growth was found in nine instances. Renal arteriography was performed in 27 of these patients and the demonstration of the renal vein by this method has been compared with that of caval venography. The incidence of collateral veins on the arteriogram and the finding of impaired renal function as an index of renal vein occlusion has been discussed.

Adenocarcinoma↗
PubMed1979

Interstitial cystitis.

Twenty histologically proven cases of interstitial cystitis were investigated in an attempt to find evidence of a possible autoimmune mechanism in its aetiology. HLA tissue typing was performed, autoantibody profiles were obtained, and a questionnaire of the patients' past history completed, especially with respect to other autoimmune conditions. An experiment was designed to test the effect of sodium cromoglycate on rat bladders. The results are discussed in relation to the management of patients with interstitial cystitis.

Aged↗
PubMed1977

Successful bone marrow transplant for Fanconi's anaemia.

A 15-year-old boy with Fanconi's anaemia, who required four units of blood each month, received a bone marrow graft from his 9-year-old brother, who has HLA identical and compatible on mixed lymphocyte reaction. Considerable immunosuppression was used and bacterial infection was prevented by vigorous decontamination in a Vickers-Trexler isolator. After the graft the patient's blood counts remained satisfactory for nine months, but it took six months before qualitative immune function was normal.

Adolescent↗
PubMed1977

HLA antigens in sarcoidosis.

The HLA antigens were identified in sixty-five patients with sarcoidosis, comprising forty-five with uveitis, twelve with erythema nodosum and eight with arthritis. In the group with arthritis, B8 was present in seven of eight (P = 0-0016) and the haplotype 1,8 in five of eight (P = 0-0053). A1 was present in 44% with uveitis (P = 0-04). There was no other significant disturbance of antigen frequencies in uveitis or in erythema nodosum, but it was noteworthy that B27 was present in only two patients with uveitis.

Arthritis↗
PubMed1976

Diminished mixed lymphocyte reaction in ankylosing spondylitis, relatives, and normal individuals all with HL-A 27.

The mixed lymphocyte reaction against a pool of blocked lymphocytes was studied in individuals with and without HL-A 27, including controls, spouses, parents, sibs, and offspring, and patients with ankylosing spondylitis. In controls without HL-A 27 the mean increment of counts perminute was 46,630 compared with 29,860 in asymptomatic controls and relatives with HL-A 27 and 26,277 in spondylitic patients with HL-A 27. The mean response was also reduced to 34,902 in sibs and offspring without HL-A 27, and to 20,916 in three patients with spondylitis in the absence of HL-A 27.

HLA Antigens↗
PubMed1976

HLA frequencies in less common arthropathies.

There was no increased incidence of HLA-B27 in patients suffering from rubella arthropathy, when compared to both the Westminster controls and a group of patients with arthritis secondary to dysentery who nearly all possessed this allele.

Arthritis, Infectious↗
PubMed1976

The elective treatment of graft-versus-host disease following a bone marrow graft from a father to a son with severe combined immunodeficiency.

The details of the first successful bone marrow graft from father to son are described. The recipient developed an acute graft-versus-host reaction on the seventh day which was successfully treated with anit-lymphocyte globulin and methotrexate. This regime did not destroy the graft and the child is alive and well with normal immunological function some 2 1/2 years later.

Antilymphocyte Serum↗
PubMedDate not supplied

Effect of cigarette smoking on lipids, lipoproteins, blood coagulation, fibrinolysis and cellular components of human blood.

SUMMARY: A group of 187 volunteers aged 30-60 years were divided into heavy and light smokers and non-smokers. Heavy smokers smoked over 20 cigarettes per day and light smokers between 5-15, averaging about 7 cigarettes per day. (1) Compared with male non-smokers (N.S.), the male heavy smokers (H.S.) had a higher fasting serum turbidity, higher levels of cholesterol (mainly confined to the ester cholesterol fraction) and higher levels of serum phospholipids and triglycerides. In this H.S. group, the esterified fatty acid index (EFI) of beta and pre-beta lipoprotein were also raised as shown by paper and cellulose acetate electrophoresis. The Stypven clotting times were also shorter than for the N.S. group. (2) Increased levels of triglycerides, pre-beta EFI, longer fibrinolysis times and shorter Stypven times were recorded in the group of female heavy smokers. Changes in cholesterol levels, beta EFI, phospholipids and fasting serum turbidity were not seen in this group. (3) Again, in the male heavy smokers, haematocrit and haemoglobin levels and mean corpuscular volumes were raised. The white cell count (WBC) was very significantly raised and a differential count indicated rises in neutrophils and lymphocytes. These changes were not significant in the female H.S. group. (4) No significant changes were found in the group of light smokers but a trend was usually seen that suggested that these changes were influenced by the number of cigarettes smoked.

Adult↗