5-azacytidine-induced decrease in the frequency of Barr body in human fibroblasts.
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Azacitidine↗
Biomedical subjects
D C Luckett
Publications and source records attributed to D C Luckett.
Spontaneous and induced sister-chromatid exchanges in cultured mosquito cells.
Four mosquito cell lines derived from species of the genus Aedes were examined for spontaneous and induced sister-chromatid exchanges by the BrdU-labelling method. The five agents tested for SCE induction were: ethyl methanesulphonate (EMS), methyl methanesulphonate (MMS), ethyl nitrosourea (ENU), methyl nitrosourea (MNU), and 5-bromo-2-deoxyuridine (5-BrdU). It was found that there is significant interspecific and even intraspecific variation in the spontaneous frequency of SCEs. All five agents were effective inducers of SCEs and the induction was dose-dependent within the range of chemical concentrations used. This study shows that the mosquito cell lines may provide a simple and reliable in vitro assay system for testing the genotoxic effects of various agents, particularly on insects.
Aedes↗
Embryonic characteristics in superovulated mouse strains. Comparative analyses of the incidence of chromosomal aberrations, morphological malformations, and mortality of embryos from two strains of superovulated mice.
Superovulation was induced in an inbred (C3HeB/FeJ) and a random-bred (CD-1) strain of mice and pregnant females were serially sacrificed from 6-15 days of gestation. All embryos were examined chromosomally and by external morphology. A G-banding technique was used to detect specific chromosomal constitutions. The common types of chromosomal aberrations were monosomies and trisomies. Our results showed that the proportion of chromosomally abnormal embryos was significantly greater in the superovulated group of inbred mice, than in the spontaneously ovulating inbred group, an observation not made in the random-bred mice. In each strain, superovulation yielded a significantly higher proportion of morphologically malformed embryos, but no significant difference between strains with respect to this criterion was noted after superovulation. There was a significant increase in the post-implantation embryonic mortality in the random-bred strain but not in the inbred strain following superovulation. This study indicates that each strain has its intrinsic pattern with regard to the fate of embryos following superovulation.
Animals↗