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Biomedical subjects

D C Moore

Publications and source records attributed to D C Moore.

At least 19 recordsLinked to original sources
PubMed2001

Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis.

This study was performed to investigate the effect of loading on the biology of newly forming bone during limb lengthening. Unilateral 2.0 mm femoral lengthenings were performed in 20 male Sprague Dawley rats. Half (n = 10) of the animals were allowed to bear weight freely, while the other half were prevented from weight-bearing via an ipsilateral through-knee amputation. The animals in each group were sacrificed after one (n = 5) or four (n = 5) days of consolidation (post-operative days seven and 10, respectively). In situ hybridization for osteocalcin and collagen I, and antibody staining for collagen II and BMP 2/4 were used to evaluate the molecular influence of loading. There was more new bone in the distraction gap of the weight-bearing animals than there was in the non-weight-bearing animals. BMP 2/4 expression, and the messages for collagen I and osteocalcin, were more abundant in tissue from the weight-bearing animals; collagen II was higher in the non-weight-bearing animals. This suggests that early regenerate tissue is capable of responding to loading, and that weight-bearing appears to stimulate intramembranous ossification. These findings support the concept of early weight-bearing after limb lengthening.

Animals↗
PubMed2000

The effect of tubularization on the mechanical properties of patellar tendon grafts.

To determine the effect of tubularization on the prefailure mechanical properties of bone-patellar tendon-bone autografts used for anterior cruciate ligament repair, 10 bovine bone-patellar tendon-bone grafts were tested in tension before and after tubularization with running suture. The testing protocol involved a 5-N preload, 10 preconditioning cycles to 200 N, and a final test cycle to 950 N at 1000 N/sec. Five of the grafts were tested first as harvested (flat) and then again following tubularization. The remaining five grafts were tubularized prior to the initial testing, and final testing was done with the suture removed. Raw testing data were reduced to determine the amount of stretching associated with preconditioning, as well as laxity and stiffness of the preconditioned grafts. Tubularized grafts stretched significantly more than flat grafts during preconditioning: 3.5 times as much after the first preconditioning cycle (3.8+/-1.9 mm versus 1.1+/-0.78 mm) and 3.1 times as much after 10 cycles (5.0+/-2.1 mm versus 1.6+/-0.9 mm). There was no statistically significant difference in the stiffnesses of the tubularized and flat grafts, nor did tubularization have an effect on graft laxity. Interestingly, there was a slight increase in laxity the second time each graft was tested, regardless of whether the graft was flat or tubularized when it was first tested. These results highlight the importance of preconditioning patellar tendon grafts before fixation, especially those that have been tubularized.

Animals↗
PubMed1999

Cloning and molecular characterization of plasmid-encoded antigens of Borrelia burgdorferi.

Thirteen independent clones that encode Borrelia burgdorferi antigens utilizing antiserum from infection-immune rabbits were identified. The serum was adsorbed against noninfectious B. burgdorferi B31 to enrich for antibodies directed against either infection-associated antigens of B. burgdorferi B31 or proteins preferentially expressed during mammalian infection. The adsorption efficiency of the immune rabbit serum (IRS) was assessed by Western immunoblot analysis with protein lysates derived from infectious and noninfectious B. burgdorferi B31. The adsorbed IRS was used to screen a B. burgdorferi expression library to identify immunoreactive phage clones. Clones were then expressed in Escherichia coli and subsequently analyzed by Western blotting to determine the molecular mass of the recombinant B. burgdorferi antigens. Southern blot analysis of the 13 clones indicated that 10 contained sequences unique to infectious B. burgdorferi. Nucleotide sequence analysis indicated that the 13 clones were composed of 9 distinct genetic loci and that all of the genes identified were plasmid encoded. Five of the clones carried B. burgdorferi genes previously identified, including those encoding decorin binding proteins A and B (dbpAB), a rev homologue present on the 9-kb circular plasmid (cp9), a rev homologue from the 32-kb circular plasmid (cp32-6), erpM, and erpX. Additionally, four previously uncharacterized loci with no known homologues were identified. One of these unique clones encoded a 451-amino-acid lipoprotein with 21 consecutive, invariant 9-amino-acid repeats near the amino terminus that we have designated VraA (for "virulent strain-associated repetitive antigen A"). Since all the antigens identified are recognized by serum from infection immune rabbits, these antigens represent potential vaccine candidates and, based on the identification of dbpAB in this screen, may also be involved in pathogenic processes operative in Lyme borreliosis.

Adhesins, Bacterial↗
PubMed1999

Torsional stiffness of three spine constructs for thoracic scoliosis.

The standard two-rod thoracic scoliosis construct is bulky in the thin, young patient. A single-rod construct with hooks at every level was developed. Five calf spines were tested measuring angular displacement before and after instrumentation using three scoliosis constructs: a standard two-rod construct, a single-rod construct, and a single-rod construct with crossover hooks. Rotational displacements and stiffness were compared. There was no statistical difference in rotational displacement or stiffness among the three constructs. All three constructs were significantly stiffer in torsion than the uninstrumented control. The single-rod construct and the crossover construct may offer the spine surgeon additional options in instrumenting thoracic scoliosis, if our preliminary studies are verified by others.

Animals↗
PubMed1998

Scapholunate ligament reconstruction using a bone-retinaculum-bone autograft: a biomechanic and histologic study.

A new surgical technique has been recently described that involves reconstruction of the dorsal aspect of the scapholunate ligament (DSLL) with a bone-retinaculum-bone (BRB) autograft preparation from Lister's tubercle. In this study, the mechanic and histologic properties of the 2 tissues were compared. The BRB and DSLL specimens were harvested from 6 fresh-frozen human cadaveric forearms. The specimens were measured and then tested in tension with an MTS 810 servohydraulic materials testing machine at a rate of 10 mm/min. The BRB autograft was significantly weaker than the DSLL. However, because the mean cross-sectional area of the DSLL was more than 3 times as large as that of the BRB autograft, the failure stress (failure force/cross-sectional area) of the BRB autograft was not significantly different from that of the DSLL. Histologically, the DSLL and BRB autograft were also similar. These findings suggest that the BRB autograft may be appropriate graft material for scapholunate ligament reconstruction, but that structural parity with DSLL will ultimately depend on remodeling and hypertrophy during healing. This also highlights the importance of using a large BRB autograft to approximate the strength of the DSLL as much as possible, and that the BRB autograft must be protected postoperatively as it heals and remodels.

Aged↗
PubMed1998

Lowering of p27Kip1 levels by its antisense or by development of resistance to 1,25-dihydroxyvitamin D3 reverses the G1 block but not differentiation of HL60 cells.

Cyclin-dependent kinase inhibitors are proteins with functions which appear to involve regulation of cell cycle traverse, and have been suggested to have a role in cell differentiation. However, there is as yet no rigorous proof that this is the case. We have addressed the participation of one of these inhibitors, p27Kip1, in the induction of differentiation and the subsequent G1 block induced in HL60 cells by 1,25-dihydroxyvitamin D3 (1,25D3). First, it was noted that sublines of HL60 cells able to grow rapidly in the presence of 1,25D3 have protein levels of p27Kip1 lower than the levels in cells subjected to 1,25D3-induced growth inhibition, but higher than in untreated parental cells. In contrast, there was no discernible relationship between the levels of p27Kip1 and the expression of differentiation markers. Further, HL60 cells treated with 1,25D3 and an oligonucleotide antisense, but not mismatched, to p27Kip1 showed an almost complete elimination of the 1,25D3-induced G1 block, but no decrease in the expression of differentiation markers. Similar results were obtained following transient transfection with an expression vector bearing the entire p27Kip1 coding sequence in the anti-sense orientation. This is the first direct demonstration that p27Kip1 plays a role in the 1,25D3-induced G1 arrest, and that partial reduction in its levels has no effect on the induction of differentiation in HL60 cells.

Calcitriol↗
PubMed1997

Restoration of pedicle screw fixation with an in situ setting calcium phosphate cement.

STUDY DESIGN: Pedicle screws were pulled out of human cadaveric vertebrae before and after augmentation with polymethylmethacrylate or in situ-setting calcium phosphate cement. The fixation strength of screws augmented with calcium phosphate cement was compared with that of screws augmented with polymethylmethacrylate. OBJECTIVES: To determine whether a new in situ-setting calcium phosphate cement might be suitable for augmenting the fixation of pedicle screws. The principle objective was to compare the pull-out resistance of screws augmented with calcium phosphate cement with the pull-out behavior of screws augmented with polymethylmethacrylate. Polymethylmethacrylate augmentation was chosen as the standard because of its current clinical use. Five types of screws were tested to determine whether screw design had an effect on the efficacy of augmentation. SUMMARY OF BACKGROUND DATA: Although many factors affect the pull-out resistance of pedicle screws, a key determinant of their performance is the strength of their attachment to the spine. In elderly, osteopenic patients, the screw-bone interface is especially at risk for stripping during insertion or pull-out after surgery. In these patients, polymethylmethacrylate has been used to augment pedicle screw fixation, although its use is not without risk. In situ-setting calcium phosphate cements may provide an alternative to polymethylmethacrylate in this application. Like polymethylmethacrylate, calcium phosphate cements can be injected into the prepared screw hole. They have the added advantage of being resorbed and replaced during healing and normal bone remodeling. METHODS: Thirty human lower lumbar vertebrae (L3-L5) were implanted bilaterally with one of five types of pedicle screws (n = 6 for each screw type). The screws were pulled out 3.0 mm at 0.25 mm/sec with a servohydraulic materials testing machine. The 3.0-mm pull-out distance, which was slightly longer than one thread pitch, was designed to strip the screw-bone interface but to leave the pedicle otherwise intact. After the initial testing, the screws in each vertebrae were removed, and the screw tracks were filled with 2.0 cc of polymethylmethacrylate (one side) or calcium phosphate cement (contralateral side). After augmentation, the screws were reinserted, and the cements were allowed to harden for 24 hours. Postaugmentation testing followed the protocols for preaugmentation testing, and the pull-out resistance of screws augmented with calcium phosphate cement was compared with the pull-out resistance of screws augmented with polymethylmethacrylate. RESULTS: Mechanically, calcium phosphate cement compared favorably with polymethylmethacrylate for augmenting pedicle screws. Both restored the strength of the screw-bone interface: across all screw types, the average increase in pull-out strength was 147% with polymethylmethacrylate augmentation and 102% with calcium phosphate cement. There were no significant differences because of screw type with either type of augmentation. CONCLUSIONS: The in situ-setting calcium phosphate cement investigated in this study compared favorably with polymethylmethacrylate in a single-cycle, pull-out test of augmented pedicle screws in senile trabecular bone. With further evaluation, this cement may offer an alternative to polymethylmethacrylate for the enhancement of pedicle screw fixation clinically.

Bone Cements↗
PubMed1997

Hip screw augmentation with an in situ-setting calcium phosphate cement: an in vitro biomechanical analysis.

OBJECTIVES/HYPOTHESIS: This study was performed to determine whether a new, in situ-setting calcium phosphate cement would have sufficient mechanical integrity to reinforce compression screw fixation of unstable intertrochanteric fractures. We compared the cut-out resistance of screws augmented with calcium phosphate cement to the cut-out resistance of screws augmented with polymethylmethacrylate (PMMA). We used PMMA as the standard for comparison because it is currently used clinically. Our hypothesis was that initial fixation strength with PMMA and calcium phosphate cement augmentation would not be significantly different from one another. STUDY DESIGN: Cut-out testing of compression hip screws in paired human cadaveric proximal femurs was performed before and after augmentation with PMMA or calcium phosphate cement. Bilateral testing was performed to allow pairwise comparisons of the materials used for augmentation, and repeated testing was done to provide an internal control for the effects of bone quality. The initial fixation of screws augmented with calcium phosphate cement was compared with that of screws augmented with PMMA. METHODS: Ten paired human femurs (mean age, 75 +/- 9.2 years) were implanted with Richards AMBI compression hip screws. Basicervical osteotomies were then performed, yielding isolated proximal fragments for mechanical testing. Preaugmentation cut-out tests were performed under displacement control, with cut-out continuing to five millimeters at two millimeters per second. The screws were then removed, and the screw tracks were filled with 2.0 cubic centimeters of PMMA (one side) or calcium phosphate cement (contralateral side). After augmentation, the screws were reinserted and the cements were allowed to harden for twenty-four hours. Postaugmentation testing followed the protocols for preaugmentation testing, and the initial fixation strength of screws augmented with calcium phosphate cement was compared with the initial fixation strength of screws augmented with PMMA using a two-way repeated measures analysis of variance. RESULTS: The cut-out behavior of screws augmented with calcium phosphate cement was not significantly different from the cut-out behavior of screws augmented with PMMA. With calcium phosphate cement, yield strength increased by 15.8 percent (from 1,354 +/- 632 newtons to 1,568 +/- 320 newtons); with PMMA, the yield strength increased by 26.8 percent (from 1,477 +/- 526 newtons to 1,834 +/- 225 newtons). However, only the increase with PMMA augmentation was significant at p < 0.05). The energy to yield increased significantly (41 percent, p < 0.05) with both types of augmentation (from 2,399 +/- 1,186 newton-millimeters to 3,378 +/- 857 newton-millimeters for calcium phosphate cement, and from 2,635 +/- 1,113 newton-millimeters to 3,741 +/- 426 newton-millimeters for PMMA), whereas the stiffness increased only slightly with PMMA augmentation (6.2 percent, from 481 +/- 180 newtons per millimeter to 511 +/- 92 newtons per millimeter) and fell slightly with calcium phosphate cement augmentation (10 percent, from 457 +/- 201 newtons per millimeter to 411 +/- 663 newtons per millimeter). CONCLUSIONS: The in situ-setting calcium phosphate cement investigated in this study compared favorably with PMMA in a single-cycle cut-out test of augmented compression hip screws in senile trabecular bone. Our results suggest that these materials may have promise as substitutes for PMMA in the salvage of compression hip screw fixation in elderly osteopenic patients with complex intertrochanteric fractures and that further study of their use in this application is warranted.

Aged↗
PubMed1996

The role of pediculolaminar fixation in compromised pedicle bone.

STUDY DESIGN: This in vitro study analyzed the effects of a supralaminar hook on pedicle screw fixation in compromised pedicle bone. OBJECTIVES: To determine the ability of pediculolaminar fixation to restore pedicle screw pull-out strength after stripping of senile pedicle bone. SUMMARY OF BACKGROUND DATA: Despite improvements in pedicle screw design, the bone-screw interface remains the "weakest link" in pedicle screw fixation. This interface is especially vulnerable in osteoporotic bone previously instrumented pedicles, and at the ends of long instrumentation constructs. METHODS: Side-to-side testing between a pedicle screw and a pedicle screw supplemented with a supralaminar hook (pediculolaminar fixation) was performed in human cadaveric lumbar vertebrae. Comparisons were made for intact and compromised pedicle bone. RESULTS: Pediculolaminar fixation restored 89% of intact pedicle screw pull-out strength whereas the pedicle screw alone restored only 19% of intact pull-out strength. The role of pediculolaminar fixation was greatest in weaker bone. Significant differences were noted in energy to failure and post-failure energy. In intact bone, the pediculolaminar construct did not increase pull-out strength or energy to failure, although it did have a greater post-failure energy. CONCLUSIONS: Pediculolaminar fixation can augment pedicle screw fixation in pedicle bone compromised by previous stripping or significant osteoporosis or both.

Aged↗
PubMed1996

Natural course of 'subclinical' hypothyroidism in childhood and adolescence.

OBJECTIVES: To describe the natural course of "subclinical" hypothyroidism due to autoimmune thyroiditis in children and adolescents, and to determine whether, like euthyroid childhood autoimmune thyroiditis, it would also run a more benign course in this age group than in adults. DESIGN: Case series. SETTING: Pediatric endocrine clinic in a tertiary medical center. PATIENTS: Eighteen patients (age range, 5 to 19 years) with juvenile autoimmune thyroiditis and an initially elevated serum thyrotropin (thyroid-stimulating hormone) concentration were followed up from documentation of the elevated serum thyrotropin concentration for a mean of 5.8 years. Eleven patients never received treatment, and seven were followed up after discontinuation of therapy. MAIN OUTCOME MEASURES: Changes in the serum thyrotropin and thyroxine concentrations and thyroid gland size, as well as signs and symptoms of hypothyroidism, were monitored throughout the observation period. RESULTS: The mean duration of observation during which the patients did not receive any therapy was 47.3 months. At the end of the observation period, seven patients were euthyroid, 10 continued to have an elevated serum thyrotropin concentration with a normal serum thyroxine concentration, and one became hypothyroid. CONCLUSIONS: "Subclinical" juvenile hypothyroidism may be a benign and remitting process in many older children and adolescents. In view of the undefined risks of levothyroxine sodium therapy, it may be possible to follow up expectantly selected younger patients with a minimally elevated serum thyrotropin concentration, rather than to treat them empirically.

Adolescent↗