Biomedical subjects
Publications and source records attributed to D Chappard.
Black and white microphotography makes use of several colored filters for an increased contrast. These filters are sometimes expensive and do not correspond exactly to the stain to be masked on the negative. Our specific colored filters contain a sufficient and adjusted amount of the dye used in the staining technic in an Agar medium.
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In some pathologic cases, areas of woven bone can be observed in and around human adult bones. This plexiform neo-osteogenesis looks like fetal bone. The mean osteoplastic surface was quantified in these different kinds of bony tissues. The formation of this plexiform neo-osteogenesis is discussed.
Measure of nuclear areas was performed on buccal smears by the Quantimet 720 image analysing computer. Five different staining procedures were used and we consider that toluidin blue at pH 4 is the best for a fine quantitative caryometry.
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A quantimetric study of vaginal superficial cells is done every day of the sexual cycle on five normal women (no pill) with a cervical smear of type 2 (Papanicolaou classification). Our results show that nucelo-cytoplasmic ratio for 98% of the cells varies between 0.0040 and 0.0210. This level is independant of age and cycle duration and is proposed as reference for later studies of women under pill or with cervico-vaginal dysplasia and cancer.