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D Chiarasini

Publications and source records attributed to D Chiarasini.

5 recordsLinked to original sources

Scanning electron microscopy of femoral ossification in the human foetus.

The ossification of human femoral bone was studied in 10 fetuses aged from 8 to 40 weeks, and in a 3 1/2 month-old child. After dissection and radiographic examination, each femur was sectioned in a sagittal plane. One part was processed for routine histology, the remaining one for scanning electron microscopy. By comparing the radiographic, light microscopic and scanning electron microscopic pictures, it is possible to precise the pattern of ossification and to define 3 main periods during human fetal development (pure cartilage, metaphyseal and diaphyseal ossification, constitution of a cortex and a medulla in the diaphysis).

Embryonic and Fetal Development↗

[Effect of collagenase, hyaluronidase and pronase, alone or combined with cationic markers, on the renal filtration barrier in the rat].

The authors investigate the effect of collagenase, hyaluronidase and pronase, used either alone or associated with cationic tracers such as Alcian Blue, Ruth enium Red and Polyethyleneimine on the Rat glomerular filtration barrier. Collagenase appears to promote the perviousness of the three constitutive layers of the glomerular basement membrane. The effects of hyaluronidase and pronase seem to be specific and permit to suppose that the glomerular basement membrane supports several types of anionic sites. The affinity of cationic tracers seems to fluctuate according to the constitution of these sites.

Alcian Blue↗