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Biomedical subjects

D Cordonnier

Publications and source records attributed to D Cordonnier.

At least 91 records · Page 5Linked to original sources
PubMed1979

Ultrastructural study of human IgG and IgG-IgM crystalcryoglobulins.

Thirty human cryoglobulin precipitates obtained from 21 patients were fixed and examined by electron microscopy; following biochemical isolation and identification. This study showed that the fine structure of cryoprecipitates depends on the involved immunoglobulins and on their respective quantities. Monoclonal IgG kappa 1 or 3 cryoglobulins with antibody activity form crystalling precipitates of 22-nm diameter rods and annuli. When polyclonal, they form 6-nm-wide filaments. Mixed IgG and IgM cryoprecipitates appear as cylindric and annular bodies with an internal diameter of 12 nm and a total diameter of 62 nm. When IgM predominates over IgG, globular condensations with a diameter of 30 nm are seen. Mixtures in which the IgG is more abundant than the IgM include fingerprint-like periodic condensations.

Antibodies↗
PubMed1979

[Cryoglobulin analysis by ultracentrifugation in density stabilized gradient followed by immunodiffusion (author's transl)].

A technique is described which combines ultracentrifugation in a density gradient stabilized by gelification, with agar gel immunodiffusion. The use of this procedure to determine sedimentation parameters and antigenic determinations is presented and illustrated with the diagramms obtained in the identification of various cryoglobulins.

Centrifugation, Density Gradient↗
PubMed1978

[Deafness due to renal failure. Clinicopathological study (author's transl)].

In this series of 54 cases of chronic renal failure, the largest number of curves showed a curve of a presbyacousia type (3/4 of the total). This hypoacousia may be related to the length of time for which the renal pathology has existed and, parat from group 0 (patients treated without haemodialysis nor grafting), may be correlated with the curve of the speed of nervous conduction. This latter correlation is particularly close in patients with a strictly isolated renal disease. The aetiology of this deafness would appear to be related to the premature aging provoked by chronic renal failure. The method of surface preparation applied to 5 petrous temporal bones from 3 patients was felt to be of particular value in the study of this pathology. Whilst one case of unilateral sudden deafness of vascular aetiology is reported, no vascular changes were seen in the other petrous temporal bones. Case 7-11 was particularly demonstrative since bilateral deafness with a rapidly progressive descending occurred in the presence of changes in the pre-ganglionic cochlear fibres charaterised by demyelinisation. This was associated with a cell loss of approximately 25% in the spiral ganglion and less marked demyelinisation of the fibres of the vestibular nerve.

Adult↗
PubMed1975

Mixed IgG-IgM cryoglobulinemia with glomerulonephritis. Immunochemical, fluorescent and ultrastructural study of kidney and in vitro cryoprecipitate.

Comparative studies of renal biopsy specimens and in vitro cryoprecipitate were carried out in a patient with mixed immunoglobulin G (IgG)-immunoglobulin M (IgM) cryoglobulinemia associated with glomerulonephritis. The IgM isolated from the cryoprecipitate was an antibody with anti-IgG activity. Proliferative endocapillary glomerulonephritis was found in the kidney, with large amorphous deposits in the capillary walls. On immunofluorescent examination, these deposits contained IgG and C3. Ultrastructural studies of both cryoprecipitate and glomerular deposits revealed unusual structures designated as "cylindrical or annular bodies." The morphologic characteristics of these bodies were exactly the same in the kidney and in the cryoprecipitate. These findings suggest an identity between the glomerular deposits and the circulating cryoglobulin, supporting the hypothesis that the glomerulonephritis reported here is an immune-complex disease.

Aged↗