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D Critchley

Publications and source records attributed to D Critchley.

6 recordsLinked to original sources
PubMed1995

Comparisons between the effects of 5-HT and DL-fenfluramine on food intake and gastric emptying in the rat.

5-Hydroxytryptamine (5-HT) dose-dependently increased gastric emptying in rats, whereas DL-fenfluramine produced a biphasic dose response curve for gastric emptying. Thus, fenfluramine increased gastric emptying at a dose of 0.1 mg/kg but decreased it at doses of 1 and 3 mg/kg. Both 5-HT and DL-fenfluramine produced significant decreases in food intake. As 5-HT produced opposing effects on gastric emptying and food intake, it appears that the anorectic effect of 5-HT may not depend on changes in the rate of gastric emptying. In contrast, doses of DL-fenfluramine that decreased feeding also decreased gastric emptying, which suggests that reduced food intake may at least partly result from decreased gastric emptying. Taken together, these results suggest that the effects of 5-HT and DL-fenfluramine on feeding and gastric emptying may be mediated by different mechanisms.

Animals↗
PubMed1995

Effect of hospitalization on conventional and 24-hour blood pressure.

Blood pressure (BP) levels, particularly in hypertensives, often fall on admission to hospital. Whether such a BP fall also occurs for BP measured automatically without the presence of medical and nursing staff and hence without the alerting reaction has not previously been studied. Twenty-two subjects, mean age 80 +/- 7 years (BP range 110-190/60-110 mmHg) underwent nurse-taken BP measurements and 24-hour non-invasive BP monitoring on admission to hospital and again after 5 days. Nurse-taken systolic BP (SBP) levels on admission fell significantly from 149 +/- 21 mmHg to 137 +/- 16 mmHg (p < 0.01) on day 2, to 131 +/- 16 mmHg on day 5 (p < 0.05). Mean diastolic BP (DBP) levels showed a less marked fall over 5 days from 87 +/- 12 mmHg to 84 +/- 11 mmHg (p < 0.05). Mean 24-hour BP levels showed no significant change from admission to day 5 (131 +/- 17/72 +/- 12 mmHg vs. 131 +/- 17/72 +/- 10 mmHg, respectively). In addition, nurse-taken SBP on day 5 was similar to mean 24-hour and daytime SBP taken on day 1 or day 5. Using shorter periods of BP monitoring gave similar mean BP levels to the full mean 24-hour BP level although BP reproducibility was poorer, even with more than 10 hours of BP monitoring. A period of automatic BP monitoring on admission to hospital will rapidly reveal a patient's sustained hospital BP level.

Aged↗
PubMed1977

Distinguishable transformation-defective phenotypes among temperature-sensitive mutants of Rous sarcoma virus.

Eight transformation-defective, temperature-sensitive (ts) mutants of the Prague strain of Rous sarcoma virus, subgroup A, have been isolated after mutagenesis with 5-bromodeoxyuridine followed by selection on the basis of focus tests. Five of these mutants, ts GI201, GI202, GI203, GI204, and GI205, exhibit properties like most previously reported isolates in that they show a temperature-sensitive response to each of a variety of transformation-specific parameters tested. Interestingly, GI201, in addition to the temperature-sensitive defect, carries a lesion that was observed as a nonconditional loss of expression of plasminogen activator protease. Three mutants, ts GI251, GI252, and GI253 have been disignated partial transformation-defective (PTD) mutants since they behave as ts mutants according to some tests for transformation and as wild type according to others. These three mutants fail to form foci at the nonpermissive temperature (41 degrees C) and art nontumorigenic in 3-week-old chickens (body temperature, 42 degrees C). The agglutinability by concanavalin A of cells infected with these mutants shows a definite temperature sensitivity, as do the rate of 2-deoxyglucose uptake and the disappearance of the 250, 000-dalton normal cell glycoprotein (large, external, transformation sensitive [LETS]). Although the PTD mutant-infected cells, unlike cells infected with other transformation mutants, exhibit a cell-bound plasminogen activator protease at the nonpermissive temperature, this activator is not detectable as a free protease in the medium, as it is with wild-type, virus-infected cells. The PTD mutants behave like the wild-type parent in their ability to induce transformed growth properties in the infected cells, i.e., growth beyond normal cell saturation density with or without serum-supplemented medium and growth leading to colony formation in soft-agar- or methyl cellulose-containing suspension media.

Agglutination↗
PubMedDate not supplied

Nurses' knowledge of nebulised therapy.

Nebulisers are commonly used in the management of chronic obstructive airways disease. A survey was therefore conducted to ascertain what nurses knew about this form of therapy. Several areas of deficient knowledge were revealed, including how to clean the equipment properly, what the flow rate of gas should be and what type of gas to use. A standardised protocol for nebuliser therapy is included.

Health Knowledge, Attitudes, Practice↗