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D D Anderson

Publications and source records attributed to D D Anderson.

26 records · Page 2Linked to original sources
PubMed1981

Biological properties of "partial" transformation mutants of Rous sarcoma virus and characterization of their pp60src kinase.

We have isolated mutants of Rous sarcoma virus from an unmutagenized stock of the Schmidt-Ruppin strain of Rous sarcoma virus. These mutants induce only a "partial" transformation, and the transformation properties induced show unusual properties or combinations. Cells infected with mutant CU2 have a unique "blebby" morphology, have lost surface fibronectin, form very small colonies in soft agar, and are nearly normal with respect to adhesiveness and hexose transport. Cells infected with mutant tsCU11 have a nearly normal morphology, but grow well in soft agar. Cells infected with mutant CU12 have a fusiform morphology, intermediate levels of hexose transport and fibronectin, and form very large colonies in soft agar. Because the appearance of the different parameters of transformation is dissociated in these mutant-infected cells, these data are interpreted as supporting a model in which the transforming protein pp60src interacts with more than one primary target in generating the transformed phenotype. All of the mutants display levels of pp60src kinase activity less than that of the wild type. In the case of mutant CU12, the lower kinase activity is in part a consequence of a lower steady-state amount of pp60src inside the cell.

Animals↗
PubMedDate not supplied

The effect of intercondylar notchplasty on the patellofemoral articulation.

Using pressure-sensitive film, we measured the patellofemoral contact areas and pressures after increasing degrees of notchplasty in eight fresh-frozen cadaveric knee specimens. Each specimen was stabilized on an axial loading frame with physiologic loads applied through the quadriceps tendon at varying flexion angles. The patellofemoral joint was loaded at 90 degrees, 105 degrees and 120 degrees of knee flexion. The same measurements were then obtained after serial notchplasties of 3, 6, and 9 mm. The film was analyzed for contact areas and for contact pressures by densitometry. There was no statistical significance between contact area or pressure after notchplasties of 3, 6, or 9 mm at 90 degrees, 105 degrees, and 120 degrees of knee flexion. These data suggest that routine notchplasty does not affect the patellofemoral articulation.

Cadaver↗
PubMedDate not supplied

Insulin-like growth factor I accelerates functional recovery from Achilles tendon injury in a rat model.

We studied the effects of insulin-like growth factor I on Achilles tendon healing in a rat model. Rats were randomized into groups of six each: sham surgery, transection alone, and transection plus growth factor. Postoperatively, rats treated with growth factor had a significantly smaller maximum functional deficit and a decreased time to functional recovery than rats in the untreated groups. Biomechanical testing revealed no significant differences in the measured parameters between the treated and the untreated groups after transection. To study the mechanism of action, six additional animals received an Achilles tendon injection of the inflammatory agent carrageenan alone and six received carrageenan plus growth factor. Rats treated with growth factor did not show the inflammation-induced functional deficit experienced by the control rats. Spectrometric myeloperoxidase assays on the remaining eight rats after Achilles tendon transection demonstrated no significant difference between the untreated and the growth factor-treated groups, indicating a mechanism other than neutrophil recruitment by which the growth factor limits inflammation. Histologic studies were performed on carrageenan-injected rats at postinjection day 2 and on surgically treated rats at postoperative day 15. No gross histologic differences were seen between untreated and growth factor-treated groups. This study demonstrated that via a possible antiinflammatory mechanism, insulin-like growth factor I reduces maximum functional deficit and accelerates recovery after Achilles tendon injury.

Achilles Tendon↗