Fatal leishmaniasis in renal-transport patient.
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Adult↗
Biomedical subjects
D D Ma
Publications and source records attributed to D D Ma.
Aortic valve replacement in a patient with von Willebrand's disease.
Few cases have been reported to date of cardiac surgery performed on patients with von Willebrand's disease. This report details the management of such a patient, who underwent aortic valve replacement for severe calcific aortic stenosis.
Aortic Valve↗
Differential cytotoxicity of deoxyguanosine and 8-aminoguanosine for human leukemic cell lines and normal bone marrow progenitor cells.
The inhibitory effect of deoxyguanosine (GdR) alone or in combination with the purine nucleoside phosphorylase (PNP) inhibitor, 8-aminoguanosine (AG) was tested on human T, B, cALL and myeloid leukaemia cell lines and on normal human bone marrow haemopoietic progenitor cells. GdR was found to be toxic to T-leukaemia cells. AG (100 microM) alone did not have any inhibitory effect, but when used with GdR (2.5 X 10(-5)M) a synergistic effect was seen towards T cells. Incubation with GdR and AG resulted in a marked decrease in cell viability (greater than 90 per cent in three and greater than 75 per cent in four of 5 T leukaemic cell lines tested at 72 h). This drug combination did not inhibit the growth of non-T leukaemic cells and was also non-toxic to normal bone marrow multipotent progenitor cells (CFU-GEMM) in vitro. Adenosine deaminase (ADA) acts consecutively with PNP in purine degradation. The addition of an ADA inhibitor, deoxycoformycin and deoxyadenosine, however, did not enhance the toxicity of GdR and AG for T cell leukaemia. The possibility of using GdR and AG for in vitro removal of residual T leukaemic blasts with the sparing of normal bone marrow cells, prior to autologous bone marrow transplantation should be further explored.
Cell Line↗
Enhanced delivery of synthetic oligonucleotides to human leukaemic cells by liposomes and immunoliposomes.
The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos alone. In addition, anti-CD32 or anti-CD2 immunoliposomes improved the delivery of oligos to leukaemic cells carrying the appropriate receptor for the specific antibody-linked immunoliposome. The uptake of oligos was twice that of the liposome or non-specific immunoliposome encapsulated oligos. These findings support the use of liposomes or immunoliposomes to deliver antisense oligos into human leukaemic cells.
Cholesterol↗
Comparison of two methods for concentrating stem cells for cryopreservation and transplantation.
Dextran sedimentation with centrifugation and semicontinuous flow centrifugation was used to concentrate marrow stem cells from collections of human bone marrow. With the dextran sedimentation method, recovery of the myeloid stem cells was 92 percent in 35 percent of the original volume, but contamination by mature cells was high. In the semicontinuous flow centrifugation method the recovery of myeloid stem cells was 82 percent in 15 percent of the original volume. Contamination with platelets, erythrocytes and neutrophils was reduced to 20.4, 29.6, and 38.6 percent respectively of the original values. It was concluded that semicontinuous flow centrifugation provides a comparatively convenient and efficient method for concentrating large collections of human bone marrow cells in a closed system.
Bone Marrow Cells↗