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Biomedical subjects

D D Mirjah

Publications and source records attributed to D D Mirjah.

5 recordsLinked to original sources
PubMed1977

Isolation and study of murine C3.

C3 was isolated in purified form from fresh murine serum and plasma by precipitation as euglobulin followed by removal of other proteins on immunoadsorbent columns bearing antibodies raised against specifically C3 depleted serum. Recovery was 30--55% and the C3 was all in its native form. Functional activity was demonstrated by fixation of the C3 on EAC142gp cells and by interaction with lymphocyte C3 receptors. Mouse C3 in plasma and after isolation had a molecular weight of 240,000. Its cleavage by classical pathway and cobra factor induced C3 convertases and by trypsin yielded a major conversion product with molecular weight not less than 210,000, the electrophoretic mobility of which differed when it was generated from isolated C3 rather than in plasma.

Animals↗
PubMed1976

Enumeration of immunoglobulin-bearing lymphocytes in whole peripheral blood.

A new method is described for the detection by immunofluorescence of lymphocyte populations in whole peripheral blood. Lymphocytes are identified by their lack of intracellular peroxidase, and the absolute number of different populations can be accurately enumerated by avoiding procedures for isolating mononuclear cells before fluorescent staining. Among twenty-two normal individuals the mean +/- s.d. percentage and absolute number of lymphocytes with stable surface immunoglobulin were as follows: IgM, 8-1+/-2-3%, 142+/-60/mu1; IgD,8-9+/-2-2%, 212+/-85/mu1;IgA, 2-2+/-1-0%, 41+/-24/mu1; IgG, 2-3+/-1-6%, 60+/-47/mu1. It was confirmed that the detection of true IgG-bearing lymphocytes depends on washing the blood at 37degrees before staining or the use of F(ab)2 anti-IgG antibodies. The proportion of lymphocytes stained by whole IgG polyvalent anti-immunoglobulin antibody, which detects both true immunoglobulin-bearing lymphocytes (B cells) and Fc receptor cells was constant (20-5+/-1-0%) both in and between individuals, but the absolute numbers (380+/-117/mu1) varied with the lymphocyte count.

Adult↗
PubMedDate not supplied

Immunological studies in inflammatory bowel disease.

Three aspects of immunological function were studied in patients with Crohn's disease and ulcerative colitis (inflammatory bowel disease): atopic status and serum IgE levels; serum concentration of C-reactive protein; and C3 activation. The incidence of atopy, assessed by prick testing with common allergens, did not differ in patients with inflammatory bowel disease from healthy controls. 12 of 39 patients with Crohn's disease and 5 of 20 with ulcerative colitis, among whom were some non-atopic subjects, had elevated serum levels of IgE. Serum levels of C-reactive protein in patients were significantly greater than normal, even in those in whom the disease was clinically quiescent. Symptomatic patients with Crohn's disease had significantly higher levels than similar patients with ulcerative colitis and in Crohn's disease the levels correlated well with an overall assessment of severity and disease activity. Although conversion of C3 was detected in fresh serum samples from inflammatory bowel disease patients and not controls, only minimal traces were present in just 7 of 89 samples of EDTA--plasma from 47 patients; this finding did not correlate with disease activity. However, there were low titres of immunoconglutinin in the sera of some patients, but not in controls, suggesting that complement activation may be occurring in vivo.

C-Reactive Protein↗