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Biomedical subjects

D D Petrunin

Publications and source records attributed to D D Petrunin.

At least 19 recordsLinked to original sources
PubMed1995

[Immunochemical studies of the system of specific proteins of the human placenta].

The levels of placental lactogen, (PL), trophoblastic beta-globulin (TBG), placental alpha-microglobulin (PAMG-1), alpha 2-microglobulin of fertility (AMGF) in the blood serum of females, amniotic liquid and tissue of the placenta during pregnancy were comparatively studied and the protein-synthesizing function in the placenta in gestosis was evaluated. An original approach to differential assessment of the placental function was developed, by using the comprehensive serological immunochemical assay for placental proteins, which was based on concurrent immunodiffuse determination of PL, TBG, and enzyme immunoassay for AMGF and PAMG-1.

Adult↗
PubMed1995

[Immunochemical studies of several proteins in angina pectoris].

A comprehensive immunochemical assay was developed for quantification of apoproteins of the atherogenic lipoproteins B, H, Lp(a) and the "acute phase proteins" with unstable angina pectoris that had varied increases in the parameters studied in comparison with their physiological measures. The significance of the proteins under test was discussed in terms of pathogenesis of atherosclerosis. The asset of a complex immunochemical assay as part of the procedure involved simplicity and accessibility for wide medical practice, providing valid information.

Acute-Phase Proteins↗
PubMed1992

[Possibility of selective binding of HIV-1 infected cells by immunosorbents].

A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.

Acquired Immunodeficiency Syndrome↗
PubMed1991

Comparative study of enzyme-linked immunosorbent assay and radioimmunoassay techniques in determining serum placental protein 14 levels in gynecologic patients.

A comparative analysis of radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques in determining placental protein 14 (PP14) levels in gynecologic patients was performed. Serum PP14 levels were assessed in the patients in the last period of gestation, during menopause and in healthy nonpregnant controls. The possible discrepancy in PP14 levels was also examined in gynecologic patients with benign and malignant tumors. Both RIA and ELISA techniques proved to be sufficiently sensitive to measure PP14 concentration.

Enzyme-Linked Immunosorbent Assay↗
PubMed1991

[Immunosorption of individual HIV proteins and virions].

Immunosorbents specifically binding native (gp160, gp120, gp41) and recombinant env proteins and HIV-I virions were synthesized on the basis of Sepharose 4B and Silica with immobilized ligands such as gamma-fraction of rabbit antiserum to HIV-I proteins and purified antibodies to env proteins of HIV-I. The possibility was shown of selective extraction of HIV-I virions and individual HIV proteins both in vitro and in vivo. The titer of virus antigens (in ELISA) after perfusion via an immunosorbent of patterns with a high content of virions and HIV-I proteins was 8 times as low as the starting titer (after perfusion via the control sorbent it was 2-fold decreased). Extracorporeal immunosorption in animals after intravenous injection of recombinant env protein permitted the latter's titer to be 5 times lower. After perfusion via the control sorbent the titer dropped by at least 20% as compared with the starting titer. The possibility of using immunosorption in multimodality therapy of AIDS is under discussion.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Protein factor of fertility in malignancies.

Serum levels of the protein factor of fertility (PFF) were measured by immunoenzymatic assay with a sensitivity of 4 ng/ml. The normal concentration of PFF was within 8-20 ng/ml in males and 20-40 ng/ml in females. Elevated levels of PFF--from 50 to 400 ng/ml--were found in patients with ovarian tumors (19%), cancer of the uterine body (25%) and tumors of the uterine cervix (37%).

Animals↗
PubMed1990

[Leukocyte thermostable alpha globulin immunochemical test in the evaluation of the functional state of human leukocytes].

For detection of functional State of Human Leucocytes incubation test was proposed. From the levels of LTAG (Leucocyte thermostable alpha-globulin)--one of the cationic proteins of granules neutrophils, which excrete neutrophils from granules in the time of incubation, it is possible to estimate the degree of injury of the functional state of Leucocytes from patients and in experimental systems in vitro. The immunochemical techniques were used to study the levels of LTAG.

Acute Disease↗
PubMed1990

[Detection of myeloperoxidase in eye tissues of man].

Myeloperoxidase (MPO) is an enzyme which usually detected in leukocytes of peripheral blood. Its principal function is to produce bactericidal hypochlorite-ions in reaction of hydrogen peroxide with chlor ions. The present work deals with testing myeloperoxidase activity in different eye tissues. Evolution of MPO activity has been tested by means of dianisidine reaction and immunochemical identification in extracts. High activity of MPO was found for retina and lens of healthy men and elderly people with lens opalescence. It should be noted that cellular body, cornea, aqueous humor, vitreous humor do not manifest any noticeable activity. Iris has low activity.

Adult↗
PubMed1987

[Comparative study of the immunosuppressive properties of trophoblastic beta 1-glycoprotein and placental alpha 2-microglobulin in vitro].

It has been shown that trophoblastic beta 1-glycoprotein (TBG) and placental alpha 2-microglobulin (PAMG-2) in concentrations 60-120 micrograms/ml suppresses both the inductive and proliferative phase of unidirectional mixed lymphocyte reaction in mice, as well as proliferative responses to phytohemagglutinin or pokeweed mitogen. TBG protein was more effective. The proteins were not toxic for lymphocytes.

Alpha-Globulins↗
PubMed1985

[Complex immunochemical analysis of the proteinogram and the system of soluble leukocytic antigens in children with chronic and recurrent infections].

The immunochemical study of the blood sera of children with chronic and relapsing infections has shown an increase in the content of alpha 2-macroglobulin and alpha 1-antitrypsin in the absence of significant changes in the concentration of immunoglobulins and complement components. The immunochemical analysis of the system of soluble leukocytic antigens (SLA) has revealed a decrease in the level of SLA-1 simultaneously with the presence of redundant amounts of SLA-5 and SLA-8.

Antigens↗
PubMed1985

[Complex immunochemical study of plasma proteins and soluble antigens of leukocytes in patients with ischemic heart disease during hemoperfusion therapy].

An immunochemical analysis of 16 individual plasma proteins and 8 leukocyte soluble antigens (LSA) was performed over time in 11 coronary patients after hemosorption. The concentration of plasma lipoproteins gradually returned to normal within 2-3 weeks. Leukocyte dysfunction in the patients manifested itself in change of the specific LSA concentration in hemolysates as compared to that in donors. Shortly after hemosorption a selective 2-8-fold increase in the LSA concentration in the hemolysates was noted. Response of the leukocyte system was observed in all the cases and recorded in repetition of the procedures. Individual LSA function is under study.

Adult↗