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Biomedical subjects

D D Rao

Publications and source records attributed to D D Rao.

11 recordsLinked to original sources
PubMed1992

Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block.

DNA blot analysis of the cloned rat beta 3-adrenergic receptor gene revealed unexpected restriction enzyme cleavage sites that suggested the presence of one or more introns near the end of the coding block. This region of the rat gene was mapped and sequenced and was found to contain two introns. The first intron occurs 12 amino acids from the end of the coding block, as deduced by comparison with the beta 3 receptor cDNA. Sequence analysis of the first intron indicates that it might contain enhancer elements that could be important in the adipose tissue-specific expression of this gene. The mouse and human beta 3 receptor genes have been assumed to be intronless; however, these genes contain potential splice sites that are homologous to those present in the rat gene. The relevant regions of the mouse and human beta 3 receptor cDNAs were cloned and, by comparing them to the respective genomic sequences, it was concluded that these genes also contain one or more introns. Sequence analysis of the mouse and human beta 3 receptor cDNAs indicates that they code for proteins that are, respectively, 12 and 6 amino acids larger than previously deduced from genomic clones.

Adipose Tissue↗
PubMed1990

Two forms of the rat D2 dopamine receptor as revealed by the polymerase chain reaction.

We have used the polymerase chain reaction technique (PCR) to clone the cDNA of the D2 dopamine receptor from rat striatal mRNA. Two major PCR products were produced; one product was identical to a previously published rat cDNA, while the other, more abundant product differed only by an 87-nucleotide insert located in the region of the putative third cytoplasmic loop of the D2 receptor. A PCR approach for determining message abundance was used to determine the relative message abundance of the two forms of the D2 receptor in a variety of tissues. Possible implications of the two forms of the D2 receptor for dopamine-mediated signal transduction are discussed.

Amino Acid Sequence↗
PubMed1979

RNA synthesis of vesicular stomatitis virus. VIII. Oligonucleotides of the structural genes and mRNA.

The single-stranded RNA genome of vesicular stomatitis virus (VSV, Indiana serotype, San Juan strain) yields approx. 75 RNase T1-resistant oligonucleotides ranging in size from 10 to 50 bases. Each of the five structural genes, isolated as duplex RNA molecules hybridized to complementary mRNA, contains two or more of these large oligonucleotides. One of the oligonucleotides is identified as part of the non-coding region near the 3' end of the genome. Comparison of these results with others indicate that the RNA sequence of VSV is apparently stable in the laboratory but not in the wild. RNase T1-resistant oligonucleotides are also shown for all five VSV mRN species. Whether the mRNA for these digestions are are isolated from duplex RNA molecules or as single-stranded RNA species, the oligonucleotide patterns for each mRNA are virtually identical, indicating that each mRNA is transcribed from contiguous sequences on the genome. Comparison with published oligonucleotide patterns obtained from other isolates of VSV or from VSV deletion mutants indicate that identity and changes in their genome structure can be correlated with specific structural genes.

Base Sequence↗
PubMed1979

Synthesis of a small RNA in cells coinfected by standard and defective interfering particles of vesicular stomatitis virus.

A small RNA, containing approximately 50 nucleotides, is synthesized by cells coinfected with standard vesicular stomatitis virus and its defective interfering (DI) particles. Infection of cells by standard virus or DI particles alone does not lead to synthesis of significant amounts of small RNA. The RNA is initiated at its 5' end with (p)ppXp and is not polyadenylylated at the 3' end despite a content of 51% adenosine. It has sequences complementary to the genome of a DI particle. The synthesis of the small RNA correlates with the replication of the genome of DI particles with molar ratio small RNA/genome RNA of DI particles greater than 50. When replication of DI genomes is prevented by the addition of cycloheximide or prior UV irradiation of DI particles, small RNA is not synthesized in coinfected cells. These results indicate that the small RNA is not the result of transcriptional initiation and that it may relate to interference mediated by DI particles.

Animals↗
PubMed1977

Anorexia nervosa with acute tubular necrosis treated with parenteral nutrition.

A patient with nonoliguric acute renal failure secondary to acute tubular necrosis in conjunction with anorexia nervosa is described. Parenteral feeding at a critical time has salutory effects on the biosynthesis of new protein and thereby reduces many of the hazards of azotemia. The technique of estimating endogenous acid production is applied for the first time in a severely malnourished subject and documents the retention of dietary sulfur which presumably is retained in the formation of new tissue in the recovery phase.

Acute Kidney Injury↗
PubMed1976

Renal tubular acidosis: practical guides to diagnosis and treatment.

The syndrome of renal tubular acidosis in some one of its various forms should be suspected when an infant or child has failure to thrive, metabolic acidosis, constipation, diarrhea, vomiting, anorexia, polyuria, or dehydration in infancy. Confirmatory biochemical findings include an inappropriately high urinary pH, inadequate acid excretion and/or abnormal tubular reabsorption of filtered bicarbonate. Growth can be normal when there is sustained correction of the metabolic acidosis through appropriate alkaline therapy.

Acidosis, Renal Tubular↗
PubMedDate not supplied

A study on cancer mortality in Tarapur-based atomic energy community.

Cancer mortality risks for individuals who were employed at nuclear facilities in Tarapur and for their respective family members with whom they lived were examined. Cancer deaths that occurred in this population between 1971 and 1988 were compared with death rates published by the Bombay City Cancer Registry. Risks were expressed as standardized mortality ratios (SMRs), which were computed by dividing the observed number of deaths by the expected number of deaths and multiplying this value times 100. There were 11 deaths from cancer among the employees, and this figure was too small to permit any trend analysis with respect to radiation exposures. The SMRs for all cancers and leukemia for male employees and for middle-aged male family members who were not exposed to any radiation were not statistically significant. A much larger database of person years at risk would be required to reach definite conclusions. The combined cancer risks for employees and families combined were similar to risks experienced by individuals in Bombay.

Adult↗