Biomedical subjects

D D Schramm

Publications and source records attributed to D D Schramm.

3 recordsLinked to original sources

PubMed2001

Chocolate procyanidins decrease the leukotriene-prostacyclin ratio in humans and human aortic endothelial cells.

BACKGROUND: Polyphenolic phytochemicals inhibit vascular and inflammatory processes that contribute to disease. These effects are hypothesized to result from polyphenol-mediated alterations in cellular eicosanoid synthesis. OBJECTIVE: The objective was to determine and compare the ability of cocoa procyanidins to alter eicosanoid synthesis in human subjects and cultured human aortic endothelial cells. DESIGN: After an overnight fast, 10 healthy subjects (4 men and 6 women) consumed 37 g low-procyanidin (0.09 mg/g) and high-procyanidin (4.0 mg/g) chocolate; the treatments were separated by 1 wk. The investigation had a randomized, blinded, crossover design. Plasma samples were collected before treatment and 2 and 6 h after treatment. Eicosanoids were quantitated by enzyme immunoassay. Endothelial cells were treated in vitro with procyanidins to determine whether the effects of procyanidin in vivo were associated with procyanidin-induced alterations in endothelial cell eicosanoid synthesis. RESULTS: Relative to the effects of the low-procyanidin chocolate, high-procyanidin chocolate induced increases in plasma prostacyclin (32%; P<0.05) and decreases in plasma leukotrienes (29%; P<0.04). After the in vitro procyanidin treatments, aortic endothelial cells synthesized twice as much 6-keto-prostaglandin F(1alpha) (P<0.01) and 16% less leukotriene (P<0.05) as did control cells. The in vitro and in vivo effects of procyanidins on plasma leukotriene-prostacyclin ratios in culture medium were also comparable: decreases of 58% and 52%, respectively. CONCLUSION: Data from this short-term investigation support the concept that certain food-derived flavonoids can favorably alter eicosanoid synthesis in humans, providing a plausible hypothesis for a mechanism by which they can decrease platelet activation in humans.

Adult↗

PubMed2000

A dose-response effect from chocolate consumption on plasma epicatechin and oxidative damage.

Evidence from epidemiological studies suggests that a diet high in plant foods and rich in polyphenols is inversely associated with a risk for cardiovascular and other chronic diseases. Chocolate, like red wine and green tea, is a polyphenol-rich food, primarily containing procyanidin polyphenols. These polyphenols are hypothesized to provide cardioprotective effects due to their ability to scavenge free radicals and inhibit lipid oxidation. Herein, we demonstrate that 2 h after the ingestion of a procyanidin-rich chocolate containing 5.3 mg total procyanidin/g, of which 1.3 mg/g was (-)-epicatechin (epicatechin), plasma levels of epicatechin increased 133 +/- 27, 258 +/- 29 and 355 +/- 49 nmol/L in individuals who consumed 27, 53 and 80 g of chocolate, respectively. That the rise in plasma epicatechin levels was functionally significant is suggested by observations of trends for dose-response increases in the plasma antioxidant capacity and decreases in plasma lipid oxidation products. The above data support the theories that in healthy adults, 1) a positive relationship exists between procyanidin consumption and plasma procyanidin concentration and 2) the rise in plasma epicatechin contributes to the ability of plasma to scavenge free radicals and to inhibit lipid peroxidation.

Adult↗

PubMed1998

Unaltered meiotic chromosome segregation in Drosophila melanogaster raised on a 5% quercetin diet.

Flavonoid plant pigments are an integral part of the human diet. Although potentially negative mitotic effects of flavonoids have been observed in model organisms, investigation into meiotic effects of flavonoids has been neglected. As flavonoids affect cell signalling and DNA replication, and because the flavonoid content of the human food supply is being increased, determining the effects of flavonoids on meiotic fidelity is important. Here, the effect of the human food supply's most prevalent flavonoid, quercetin, on the level of meiotic recombination and the amount of X and 4th chromosome non-disjunction in Drosophila melanogaster females was determined. This model organism was chosen since Drosophila melanogaster and Homo sapiens share a remarkable number of commonalities in the meiotic processes of oogenesis and because genetic techniques allow a detailed analysis of meiotic processes in Drosophila. No significant effect on either non-disjunction levels or the percentage distribution of exchange bivalents was observed. A significant effect was observed on the number of offspring; F1 and F2 generations of flies raised on a quercetin diet produced over 10% more progeny than flies raised on a control diet. In this investigation, high quercetin consumption by Drosophila melanogaster females did not pose a threat to meiotic fidelity.

Animals↗