Biomedical subjects
D Datta
Publications and source records attributed to D Datta.
Myoelectric prostheses in the management of Poland's syndrome. A report of 2 cases.
Two cases of Poland's syndrome are reported in whom myoelectric prostheses have been used. The rare congenital sporadic disorder of Poland's syndrome characterized by absence of pectoralis major and varying degrees of ipsilateral upper limb deficiency has been reported by various authors (Rasjad and Sutiaksa, 1991, Gausewitz et al, 1984; David, 1982; Ireland et al, 1976). Management of the upper limb deficiency depends on the degree of functional and cosmetic disability. We report two cases of Poland's syndrome where myoelectric prostheses have been successfully used. We have been unable to find any published report of the use of a myoelectric prosthesis in Poland's syndrome.
Electron affinities of opposite-parity bound states in Th-: Relativistic-configuration-interaction studies.
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Electron affinities of six bound states of Ce- formed by attachment of 6p and 5d electrons to Ce.
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Atypical case of testicular tumour.
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Possibility of formation of rare-earth negative ions by attachment of f electrons to the atomic ground state.
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Hyperfine-structure studies of Zr II: Experimental and relativistic configuration-interaction results.
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Multireference relativistic configuration-interaction calculations for (d+s)n transition-metal atomic states: Application to Zr II hyperfine structure.
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Laser-rf double-resonance measurements of the hyperfine structure in 49Ti II.
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Relativistic many-body results for the hyperfine structure of the ground state of the Ag I isoelectronic sequence.
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Prevalence of Clostridium difficile in hospitalised patients with acute diarrhoea in Calcutta.
During a 12-month period, Clostridium difficile was isolated from 38 (11.1%) of 341 hospitalised patients suffering from acute diarrhoea and from five (3%) of 172 patients as control who were admitted with illnesses other than diarrhoea. It was the sole bacterial pathogen in 31 cases. 87% of the isolates obtained from patients with diarrhoea produced neutralisable cytotoxin. None of these patients had any history of previous treatment using antibiotics. No age or gender-specific high incidence was observed. Clinical presentation in these patients revealed no distinctive pattern. Both dysenteric and diarrhoeal presentations were found, the latter being more frequent. The results suggest that Clostridium difficile by itself may be a causal agent of acute diarrhoea.
Amputation for gangrene of the limbs following severe meningococcal infection.
Five cases of severe meningococcal infection in children and young adults with gangrene leading to major amputation are described. They are associated with necrosis of the skin and soft tissue, intravascular coagulation and shock. The surgical options, prosthetic implications and the consequent rehabilitation outcome are discussed.
Responses induced by long-term toxic effects of heavy metals on fish tissues concerned with digestion, absorption, and excretion.
The present study relates to the bioassay as well as the responsiveness induced by long-term exposure to sublethal concentrations of soft acceptor heavy metals, lead (Pb, IV A) and cadmium (Cd, II B), in various fish tissues viz., stomach/intestinal bulb, intestine, liver, pancreas, and kidney of Mystus vittatus (Bloch) and Labeo rohita (Hamilton) belonging to 2 different trophic strata. Responses, however, have been recorded by histopathological and histochemical studies on the aforesaid tissues concerned mainly with digestion, absorption, and excretion. Histopathological observations depict the vacuolation, degranulation, exocytosis of the various cells including their nuclear damage. Erosion of the top plate of intestinal villi, enlargement of epithelial cells, shrinkage of glomeruli, destruction or disruption of the nephric tubular cells, etc. have also been registered. Histochemical investigations emphasize on the change in the intensity of staining reactions of biologically active macromolecules particularly amino acids of the protein component and glycogen among the carbohydrate component as well as acid, neutral and/or mixed mucopolysaccharides of the cells concerned. Toxic effects of the afore-mentioned heavy metals at the tissue level have been discussed.
Effect of inducers on metabolism of benzo(a)pyrene in vivo and in vitro: analysis by high pressure liquid chromatography.
The characterisation of metabolites formed from benzo(a)pyrene (BP) by Aspergillus ochraceus TS and effect of inducers on BP metabolism are reported. The high pressure liquid chromatographic profile of BP metabolites was similar to that of mammalian microsomes furnishing diols, quinones and phenols. The production of BP-4,5-dihydrodiol (K-region diol) by Aspergillus ochraceus TS seems to be novel and provides first report on BP metabolism by eukaryotic fungi. In control, phenols and quinones were produced in excess over dihydrodiols while the induced preparation showed the reverse order. Presumably the induction effecting production of excess dihydrodiols influenced the synthesis of epoxide hydrolase. In addition, a differential increase in BP metabolism was observed with inducers of narrow and broad specificity.
Relative efficacy of trimethoprim-sulfamethoxazole and nalidixic acid for acute invasive diarrhea.
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Studies on the kinetic sequence of in vitro ribosome assembly using cibacron blue F3GA as a general assembly inhibitor.
We have found that all E. coli ribosomal proteins strongly bind to an agarose affinity column derivatized with the dye Cibacron Blue F3GA. We have also shown that the capacity to bind the dye is lost when the proteins are organized within the structure of the ribosome or are members of pre-formed protein-RNA complexes. We conclude that the binding of ribosomal proteins to this dye involves specific protein-RNA recognition sites. These observations led us to discover that Cibacron Blue can be used to inhibit in vitro ribosome assembly at any stage of the assembly process. This has allowed us to determine a kinetic order of ribosome assembly.
Preparative ion-exchange high-performance liquid chromatography of bacterial ribosomal proteins.
We have developed analytical and preparative ion-exchange HPLC methods for the separation of bacterial ribosomal proteins. Proteins separated by the TSK SP-5-PW column were identified with reverse-phase HPLC and gel electrophoresis. The 21 proteins of the small ribosomal subunit were resolved into 18 peaks, and the 32 large ribosomal subunit proteins produced 25 distinct peaks. All peaks containing more than one protein were resolved using reverse-phase HPLC. Peak volumes were typically a few milliliters. Separation times were 90 min for analytical and 5 h for preparative columns. Preparative-scale sample loads ranged from 100 to 400 mg. Overall recovery efficiency for 30S and 50S subunit proteins was approximately 100%. 30S ribosomal subunit proteins purified by this method were shown to be fully capable of participating in vitro reassembly to form intact, active ribosomal subunits.
Rectal histology in acute bacillary dysentery.
A recent epidemic of acute Shigella dysentery in West Bengal (India) provided us with an opportunity to examine the rectal mucosal abnormalities seen in this condition. One hundred two patients were investigated using sigmoidoscopy, rectal biopsy, and rectal swab for culture. Pure culture of Shigella was obtained in 37 cases, and the rectal biopsy specimens from these patients were assessed in detail. The mean (+/- SD) duration of illness was 47.8 +/- 27.4 h (range 8-120 h), and most patients (31 of 37, 84%) had diarrhea with blood and mucus in the stools. Significant findings at histology were as follows. (a) Cellular infiltrate was predominantly round cell or mixed round cell and neutrophilic in the majority of patients (27, 73%). (b) Disorganization of crypts was seen in as many as 31 patients (84%); in most subjects the distorted architecture was mild, but in a few the defect was severe with crypt branching and dilatation. (c) In the majority of patients the inflammatory process extended to the muscularis mucosae and submucosa; edema with or without increased cellular infiltrate was seen in the muscularis mucosae in 92% and in the submucosa in 80%. (d) There was no difference in the rectal histology of patients with a short history of disease (less than 48 h) compared with those with a longer history, except for goblet cell depletion which was more in those with diarrhea for more than 48 h. (e) The mucosal abnormalities in patients with watery diarrhea were, in general, milder than in those with dysentery, although the difference was statistically not significant; 2 of 6 patients with watery diarrhea had severe colitis. (f) The mucosal abnormalities were more severe in patients with Shigella dysenteriae infection compared with Shigella flexneri.