Biomedical subjects
Publications and source records attributed to D Dimitrov.
The authors present their own attempt of miniinvasive management of pelvic inflammatory disease. Two groups of patients are compared--in the first one the women are treated only conservatively, while the second one has undergone miniinvasive surgical treatment. The preferences of combined therapy are pointed out.
This study was carried out to investigate whether 3,5-dichloro-salicylaldehyde isonicotinoyl hydrazone (SH7), an analogue of the antituberculosis drug isoniazid (INH), recently synthesized in our laboratory, could prevent isoniazid-induced liver damage. Forty-two white healthy mice were treated, once daily for 30 days, with solutions of INH, SH7 and combinations of them at doses of 7.5 mg/kg p.o. and 15 mg/kg p.o. At the end of this period, livers were harvested for histopathological analysis. The levels of lipid peroxidation products (MDA) and the activities of antioxidant defense enzymes, superoxide dismutase (SOD) and catalase (CAT), were also measured in the liver homogenates. Treatments with combinations of INH and SH7 decreased the levels of MDA, normalized the previously depressed levels of SOD and CAT and prevented isoniazid-induced liver damages. A previously demonstrated tuberculostatic and superoxide scavenger activity (SSA) of the isonicotinoylhydrazone SH7 and results from the present study thus set out a proposal for a new isoniazid/SH7 combination therapy designed to provide hepatoprotection.
The rate of the health expenses is an important factor for the fulfillment of the actions in the field of the healthcare. The means, collected from different financial sources/the National budget-98% from the necessary means, receipts out of the budget-1.5%, collected means from the population-0.5%/are spent as it follows: for the National health institutions of the municipalities-65%; for the health institutions of national level and for the central supply with some kind of drugs, serums and vaccines-17%; for the health institutions, financed directly from the International Fond-18% from the means. The main part from the means are spent for salaries and for the social security of the staff.
6-Oxo-4(2-thienyl)-2-thioxo-1,3-dihydropyrimidine-5-carbonitrile (1) was synthesized and reacted with alkyl halides to give the S-alkyl compounds (2a,b), which reacted with phosphorous oxychloride to yield (3a,b). Reaction of 3a,b with phenyl-hydrazine, hydrazine hydrate, ammonia, acid hydrazides and thiourea leads to formation of the compounds (4a,b), the dihydrazino compound derivative (5), the amino compounds (7a,b), triazolopyrimidine derivatives (9a-d), and the mercapto compounds (10a,b), respectively. Treatment of (10a) with chloroacetone gave (11a), which condensed with benzaldehyde giving the tricyclic compound (12). Also (10b) reacted with ethyl chloroacetate followed by hydrolysis and cyclization yielded (14). Heating of (5) gave (6), which reacted with sodium nitrite and/or carbondisulphide and give (15) and (16), respectively.
The meningitis and the meningoencefalitis is 29% from all of the organic diseases of the Central Nerve System. The actuality of this problem is determined by the following factors: 1. Social-only the childish group and the active group of workers among the adults are concerned; 2. The diseases are taking their course seriously with a high percentage of lethality-30%; 3. When there is untimely and inadequate therapy, there occurred additional manifestations of the disease.
Founder analysis is a method for analysis of nonrecombining DNA sequence data, with the aim of identification and dating of migrations into new territory. The method picks out founder sequence types in potential source populations and dates lineage clusters deriving from them in the settlement zone of interest. Here, using mtDNA, we apply the approach to the colonization of Europe, to estimate the proportion of modern lineages whose ancestors arrived during each major phase of settlement. To estimate the Palaeolithic and Neolithic contributions to European mtDNA diversity more accurately than was previously achievable, we have now extended the Near Eastern, European, and northern-Caucasus databases to 1,234, 2, 804, and 208 samples, respectively. Both back-migration into the source population and recurrent mutation in the source and derived populations represent major obstacles to this approach. We have developed phylogenetic criteria to take account of both these factors, and we suggest a way to account for multiple dispersals of common sequence types. We conclude that (i) there has been substantial back-migration into the Near East, (ii) the majority of extant mtDNA lineages entered Europe in several waves during the Upper Palaeolithic, (iii) there was a founder effect or bottleneck associated with the Last Glacial Maximum, 20,000 years ago, from which derives the largest fraction of surviving lineages, and (iv) the immigrant Neolithic component is likely to comprise less than one-quarter of the mtDNA pool of modern Europeans.
Telomere shortening may reflect the total number of divisions experienced by a somatic cell and is associated with replicative senescence. We found that the average rate of telomere shortening in peripheral blood mononuclear cells (PBMCs) obtained longitudinally from nine different infants during the first 3 years of life (270 bp per year) is more than fourfold higher than in adults and does not correlate with telomerase activity. These results show that the rate of telomere loss changes during ontogeny, suggesting the existence of periods of accelerated cell division. Because human immunodeficiency virus (HIV) preferentially infects actively dividing cells, our observation suggesting accelerated cell division in children may provide an explanation for some of the distinctive pathogenic features of the HIV disease in infants, including higher viral loads and more rapid progression to acquired immunodeficiency syndrome (AIDS).
The concentration of human immunodeficiency virus type 1 (HIV-1) particles in blood plasma is very predictive of the subsequent disease course in an infected individual; its measurement has become one of the most important parameters for monitoring clinical status. Steady-state virus levels in plasma reflect a balance between the rates of virions entering and leaving the peripheral blood. We analyzed the rate of virus clearance in the general circulation in rhesus macaques receiving a continuous infusion of cell-free particles in the presence and absence of virus-specific antibodies. Here we show, by measuring virion RNA, particle-associated p24 Gag protein and virus infectivity, that the clearance of physical and infectious particles from a primary, dual-tropic virus isolate, HIV-1DH12, is very rapid in naive animals, with half-lives ranging from 13 to 26 minutes. In the presence of high-titer HIV-1DH12-specific neutralizing antibodies, the half-life of virion RNA was considerably reduced (to 3.9-7.2 minutes), and infectious virus in the blood became undetectable. Although physical virus particles were eliminated extravascularly, the loss of virus infectivity in the blood reflected the combined effects of extravascular clearance and intravascular inactivation of HIV-1 infectivity due to antibody binding.
BACKGROUND: This study includes all cases of cancer among Republic of Bulgaria Air Force and civil aviation aircrew. Cancer incidence, Standardized Incidence Ratios (SIRs) and 95% confidential intervals were calculated for the pilots, using data about cancer incidence in the same age groups of the male population of the Republic of Bulgaria for the same period of time, taken from the National Cancer Register. RESULTS: The results show a considerable difference in the structure of cancer among males in Bulgaria, as compared to male pilots (r<0.0001). While cancer of the respiratory system has the greatest incidence among the civil population, cancer of the bladder has the greatest incidence among pilots, followed by testicular and skin cancer. For the period under consideration there is a lower risk of malignant diseases in pilots as compared to the rest of the population. There is a higher risk of testicular cancer only in civil aviation pilots. For Air Force aircrew there is a 10-fold higher risk of bladder cancer as compared to the rest of the population. CONCLUSION: We established an interdependence between age and cancer incidence, cancer incidence being higher among pilots in the age group from 20-40 yr. For the remaining age groups, cancer incidence among the civil population is approximately 2.5 times higher. Of the pilots with cancer, 73.53% returned to a flying career after an average of 7 mo treatment.
This is a case report on a 16-year-old girl presenting lipoma located in the temporal cerebral lobe, in conjunction with an ever increasing frequency of epileptic seizures, unresponsive to the anticonvulsive treatment carried out. A comprehensive analysis is done of the data concerning clinical course, diagnostic examinations' specificity and microsurgical removal of the lipoma with ensuing optimal postoperative results--total abatement of epileptic seizures against the background of reduced anticonvulsive therapy throughout the 3.5 years observation period. The literature survey shows that intracranial lipomas with the location described represent as casuistic rarity.
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The rate of decline of plasma HIV RNA in patients treated with anti-retroviral drugs has been postulated to reflect the half-lives of previously HIV-infected cells. Here, Zvi Grossman and colleagues argue that the observed decline is explained by the kinetics of ongoing infection cycles. Residual cell-to-cell infection that becomes increasingly difficult to block could stabilize cellular provirus reservoirs.
Most patients with chronic odontogenic sinusitis experience aeration problems which remain unresolved even after classic radical antrotomy has been performed. The aim of this study was to create a high-yield diagnostically feasible and accurate CT technique which would allow the evaluation of the degree of postoperative changes of maxillary sinus aeration and overcome the limited value of traditional imaging modalities. We studied the aeration sequelae in the maxillary sinus following antrotomy in thirty-five patients employing Sytec 3000 CT scan equipment with modern densoprofile software. We found a prevalence of 62.92% for minor complications following antrotomy--a reduction in the volume of the maxillary sinus up to 25%, whereas the more serious complications accounted for 5.72%. Conventional radiologic modalities demonstrated only 25% of these changes. Our results underscore the importance of the densoprofile CT technique as a valuable and accurate diagnostic tool in detecting the changes consistent with the postantrotomy syndrome.
Twenty-eight mass burn disasters in Bulgaria with 381 injuries in the last 27 years (1968-1994) were reviewed. Mass burn disasters were classified as follows: industrial accidents, 23, with 273 injured and 103 dead; traffic accidents, 2, with 97 injured and 30 dead; domestic accidents, 2, with 11 injured and 6 dead. Organization of first aid at the disaster site and in the regional hospitals is described, as well as the time of transport of the patients to major hospitals near the site of the accident. As a priority, the injured were transported early to the biggest center for burns and plastic surgery in the capital.
A seroepidemiological study was conducted in Kuwait to evaluate the pattern of acquisition of human parvovirus B19 by children in Kuwait and to compare it with patterns described in other regions in different climatic zones. A total of 218 serum samples from children less than 16 years of age were tested for the presence of anti-B19 IgG by enzyme immunoassay. The overall seroprevalence was 17.4%. Infants in Kuwait had low levels of maternally-acquired anti-B19 IgG (8.6%). The age of peak exposure to parvovirus B19 was 10-15 years compared with less than 10 years in England and Wales and more than 20 years in Singapore. The results of this study indicate an influence of geographic differences on transmission of parvovirus B19.
Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.
Membrane recombinant CD4 was electroinserted into the plasma membrane of red blood cells (RBCs) from four HIV patients. CD4 had been labeled with 125I before electroinsertion. The RBCs-CD4-125I were labeled with 51Cr and autotransfused to the donor patients. The hematological indexes and the P50 value of the RBCs were not modified by the electroinsertion of CD4. The life span of the RBCs was not affected by electroinsertion of CD4 (t1/2 approximately 30 days), whereas the exposed CD4 showed a kinetics of disappearance characterized by two half-life times: a short one (t1/2 approximately 1 day) and a long one approximately equal to that of the RBCs. No side effects or anti-CD4 immune responses were observed in patients over a period of 28 days. The RBC-CD4 entity appears to be long-lived and has no adverse effect in HIV patients.
p21Sdi1 (also known as Cip1 and Waf1), an inhibitor of DNA synthesis cloned from senescent human fibroblasts, is an inhibitor of G1 cyclin-dependent kinases (Cdks) in vitro and is transcriptionally regulated by wild-type p53. In addition, p21Sdi1 has been found to inhibit DNA replication by direct interaction with proliferating cell nuclear antigen. In this study we analyzed normal human fibroblast cells arrested in G0 and determined that an excess of p21Sdi1 was present after immunodepletion of various cyclins and Cdks, in contrast to mitogen-stimulated cells in early S phase. Expression of antisense p21Sdi1 RNA in G0-arrested cells resulted in induction of DNA synthesis as well as entry into mitosis. These results suggest that p21Sdi1 functions in G0 and early G1 and that decreased expression of the gene is necessary for cell cycle progression.