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Biomedical subjects

D Dumonde

Publications and source records attributed to D Dumonde.

6 recordsLinked to original sources
PubMed1992

Antibody affinity to retinal S-antigen in patients with retinal vasculitis.

Using a modified enzyme-linked immunosorbent antibody method that included dissociation of antigen antibody complexes with sodium thiocyanate, we examined the functional affinity of antibody to retinal S-antigen in 48 patients with retinal vasculitis and 46 age-matched healthy control subjects. Antibody affinity was markedly lower in patients with retinal vasculitis than in healthy subjects. Low-affinity antibody was more prevalent in acute retinal vasculitis and in patients with normal levels of circulating immune complexes. We found distinct differences between the antiretinal antibodies found in patients with retinal vasculitis and those in control subjects. The association of low-affinity antibody with normal levels of circulating immune complexes may suggest defective regulation of antiretinal autoimmunity and have important pathogenic implications.

Antibody Affinity↗
PubMed1988

Analysis of antigenic determinants of retinal S-antigen with monoclonal antibodies.

Eight murine monoclonal antibodies to retinal S-antigen were found to recognize three antigenic clusters in competitive binding experiments. Variable patterns of immunohistochemical staining of retinal sections supported the competitive binding studies. Monoclonal antibodies to two of these three antigenic clusters on S-antigen reacted by Western immunoblotting with both intact S-antigen and two cyanogen bromide cleaved peptide fragments of bovine S-antigen. The binding of the monoclonal antibodies was localized to epitopes contained on two peptide fragments of 26,000 and 18,000 molecular weight. The same peptide fragments also react with polyclonal rat or rabbit antisera to bovine S-antigen. Monoclonal antibodies to the third antigenic cluster did not react with either intact or fragmented S-antigen in immunoblotting studies.

Animals↗
PubMed1987

Immunoaffinity purification of S-antigen using monoclonal antibodies to different antigenic sites.

Retinal S-antigen (S-ag) was purified by monoclonal antibody (MoAb) immunoaffinity chromatography from soluble protein extracts of bovine and human retina. Purification of S-ag was readily achieved by affinity chromatography using four different MoAb-Sepharose 4B columns. The four antibody columns gave different recoveries with material of comparable enrichment with greater than 95% purity as judged by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The use of two different MoAbs covalently bound to Sepharose 4B and known to be directed to disparate, spacially distant epitopes on S-ag led to at least a twofold increase in recovery, with the aforementioned purity. Immunoaffinity purified S-ag retained its serological and uveitogenic properties. The high recovery of S-ag associated with this one-step procedure is preferable to conventional preparatory techniques, and enables high antigen recovery when tissue availability is limited (eg human retina).

Antibodies, Monoclonal↗
PubMed1985

Cyclosporin A in the treatment of posterior uveitis.

Cyclosporin A was used in nine patients with severe refractory posterior uveitis. Four patients had Behcet's disease, one had sarcoidosis, one had HLA B27 related arthritis and uveitis and three had idiopathic retinal vasculitis. The drug had beneficial ocular effects in all patients in the early stages of treatment with an improvement in the visual acuity and reduction in the severity of inflammation. The relapse rate and need for additional steroids were reduced in all patients. However, renal function deteriorated in seven patients to such an extent that the dose of Cyclosporin A had to be reduced with consequent relapse of uveitis.

Adult↗
PubMed1982

Sensitization of human lymphocytes in vitro. Kinetics and specificity of the response to hemocyanins.

In vitro primary sensitization of human peripheral blood T-cells to hemocyanins was detected during a 12-14 day culture period with antigen (KLH or HCH)-pulsed macrophages. Primed T-cells proliferate in secondary culture (2-3 days) when restimulated with antigen presented by macrophages. The kinetics of primary sensitization and secondary responsiveness are interrelated and are dependent on the antigen doses employed. Antigen-induced proliferation of cells sensitized in vitro is identical to proliferation of T-cells from immunized donors in terms of antigen specificity and the clonal nature of the response to antigen. Through the use of thymidine suicide techniques, distinct populations of cells responding to KLH or HCH can be demonstrated using cells from actively immunized donors or cells that have been sensitized in vitro to both hemocyanins.

Dose-Response Relationship, Immunologic↗