PubMed Health⌕ Search

Biomedical subjects

D E Bice

Publications and source records attributed to D E Bice.

76 records · Page 5Linked to original sources
PubMedDate not supplied

Neutrophil involvement in the retention and clearance of dust intratracheally instilled into the lungs of F344/N rats.

This study evaluated polymorphonuclear leukocyte (PMN) involvement in translocation of dust to bronchial lymph nodes after deposition of dust in the lungs of control and neutropenic F344/N rats. Rats were rendered neutropenic with an intraperitoneal (IP) injection of anti-rat PMN antiserum (APA); control rats were injected IP with 0.9% saline solution. Eighteen hours after IP injections, control and APA-treated rats were instilled intratracheally with 5 x 10(8) microspheres suspended in 0.9% saline solution, which caused an influx of PMNs into the pulmonary airspaces of control rats, but not of APA-treated rats. One day postinstillation (PI), 77.2% of the microspheres recovered in bronchoalveolar lavage fluid (BALF) from control rats were associated with pulmonary alveolar macrophages (PAMs) and 18.8% with PMNs; 4.0% were free. In BALF from the APA-treated rats, 66.3% of the microspheres were associated with PAMs and 0.3% with PMNs; 36.3% were free. Two days PI, about 95% of the microspheres in BALF from control and APA-treated rats were associated with PAMs; by 4 and 7 days PI, essentially 100% were with PAMs. Amounts of microspheres translocated to bronchial lymph nodes of control rats were four fold less than in the APA-treated rats on days 2, 4, and 7 PI (p < .05). The results suggest that PMNs in pulmonary airspaces of F344/N rats phagocytize dust and thereby interfere with the mechanism(s) involved in dust penetration into the pulmonary interstitium.

Animals↗
PubMedDate not supplied

Comparison of systemic and local immune responses after multiple pulmonary antigen exposures.

Both systemic and local immune responses were evaluated in five male cynomolgus monkeys after a primary and multiple secondary challenges of the lung with sheep red blood cell (SRBC) antigen. Comparisons of peripheral blood and pulmonary antibody-forming cell (AFC) responses were made after primary and multiple secondary intrapulmonary challenges with SRBC. The primary immune response to SRBC was characterized by a low lung to blood AFC ratio. This ratio, however, increased successively with each challenge, resulting in a 15-fold increase after the third exposure of the lung to SRBC. These data suggest a decreasing contribution of systemic AFC to the lung response with each successive challenge. Histopathologic and autoradiographic evaluation was done to compare a newly immunized lobe, a multiply challenged lobe, and a saline instilled lobe. Large localized perivascular lymphoid follicles appeared in the lung lobe challenged multiple times. About 17% of these large mononuclear cells had taken up 3H-thymidine compared to 5% in the newly immunized lobe and 3.8% in the control lobe. The number of proliferating cells appeared to reflect the degree of immune responsiveness within each lung lobe. Together these data support a hypothesis that after the primary immune response is established, the secondary immune response is generated by local clonal expansion of antigen-specific lymphocytes within the lung.

Analysis of Variance↗
PubMedDate not supplied

Inflammation and recruitment of immune cells into the lung.

The mechanisms responsible for recruitment of antibody-forming cells (AFC) into lung lobes exposed to antigen are not known. Because instillation of antigen induces inflammation, AFC may enter immunized lung lobes by changes in vascular permeability and/or in response to the release of mediators. The purposes of this study were to evaluate inflammatory responses produced by particulate antigens or interleukin-1 (IL-1) and to examine the recruitment of AFC and lymphocytes into the lung in response to these inflammatory stimuli. Two peaks of inflammation were observed in the lung lobes of dogs exposed to sheep red blood cells (SRBC), one at 1 day and the second around 9 days after instillation. Lymphocytes did not enter the lung during the first inflammatory response. However, lymphocytes and anti-SRBC AFC did enter immunized lung lobes during the second inflammatory response. AFC and lymphocytes also entered a lung lobe instilled with rabbit red blood cells (RRBC) at 6 days after immunization with SRBC. The observation that lymphocytes did not enter at 1 day after instillation of SRBC, but did enter the RRBC lung lobe at 1 day after instillation of RRBC, suggested that a population of lymphocytes, including AFC, were present in blood several days after immunization with SRBC that were capable of entering sites of inflammation in the lung. Instilled IL-1 was chemotactic in vivo for neutrophils and induced inflammation in the lung. However, IL-1 was not directly chemotactic for AFC or lymphocytes in vivo.

Animals↗