PubMed Health⌕ Search

Biomedical subjects

D E Lange

Publications and source records attributed to D E Lange.

At least 19 recordsLinked to original sources
PubMed1991

[Papilla-preserving flap procedure in periodontal implantation of alloplastic materials. Comparative study].

40 periodontal defects with probing depths of 5 mm in 10 patients were treated with a conventional flap or with the "papilla preservation flap". In addition, half of the defects received granular hydroxyapatite. In terms of pocket depth and probing attachment-level no significant differences were seen between the 4 groups after 3 and 6 months. Regarding the development of gingival recession, however, the papilla preservation technique has significant advantages and showed better aesthetic results.

Durapatite↗
PubMed1991

[Actinobacillus actinomycetemcomitans on oral mucosa].

The present study was carried out to delineate the parameters influencing the extracerevicular oral colonization of Actinobacillus actinomycetemcomitans. Numbers of cultivable A. actinomycetemcomitans in subgingival plaque samples, saliva, and samples from buccal and tongue mucosae were determined in 33 patients with A. actinomycetemcomitans-associated periodontitis. Subgingival samples from patients with localized juvenile periodontitis (LJP) had significantly higher levels of A. actinomycetemcomitans than samples obtained in non-LJP patients (740,000 vs. 59,000, median, p less than 0.05). Samples from buccal mucosae and saliva were infected with A. actinomycetemcomitans in 85 vs. 70%, and 77 vs. 65%, respectively. Multiple regression analysis of log 10-transformed counts of A. actinomycetemcomitans in samples from cheek as dependent variable yielded a significant influence of numbers of deep periodontal pockets and diagnosis. The occurrence of A. actionomycetemcomitans on buccal mucosa seems to reflect the overall subgingival load with the organism.

Adolescent↗
PubMed1991

[Deviations of the central ray in standardized bite-wing radiographs].

Interpretation of serial radiographs without sufficient information of projection geometry is hardly possible. We obtained 51 pairs of bite-wing radiographs in 12 patients by use of a modified filmholder (Duckworth et al. 1983). Two orthodontic wires of different profile, placed coaxially on the perpendicular, cast shadows on the film, which were differentiated by their respective shape. By placing each wire a known distance from the film plane, the angular difference between the perpendicular to the film and the actual direction of the central ray could be calculated. In 63% of pairs the angular deviation of the central rays between the serial exposures was below 0.9 degrees. The results of the present study show that utilization of this type of device can provide radiographs of sufficient quality to allow their use in image subtraction.

Humans↗
PubMed1990

Multiple periodontal abscesses after systemic antibiotic therapy.

Multiple periodontal abscesses were reported in medically compromised patients. We examined patients with a non-contributory medical history referred for the treatment of numerous periodontal abscesses. All patients had taken oral broad spectrum antibiotics 1 to 3 weeks prior to the outburst of the abscesses (8 patients: penicillin, 2 patients: tetracycline). The patients suffered from advanced periodontal disease, 82% of the examined sites showed probing depths greater than 3 mm, 56% attachment loss greater than 3 mm. Subgingival plaque samples were analysed from 2 different abscess sites. Bacteroides gingivalis (19/20), Fusobacterium nucleatum (13/20) and Streptococcus intermedius (13/20) were the most prevalent anaerobic microbiota. Strains resistant to the prescribed antibiotic were found in 55% (11/20) of the subgingival plaque samples. It was concluded that in patients with advanced periodontal disease, systemic antibiotic therapy without subgingival debridement may change the composition of the subgingival microbiota, thus favouring the outburst of multiple periodontal abscesses.

Adult↗
PubMed1990

Morphological compositions of subgingival microbiota in Actinobacillus actinomycetemcomitans-associated periodontitis.

Infrequent occurrence of spirochetes and rather low proportions of these organisms have been reported in localized juvenile periodontitis, where periodontal lesions often harbour large numbers of Actinobacillus actinomycetemcomitans, a suspected principal periopathogen strongly implicated in the pathogenesis of this and other forms of chronic periodontitis. We studied the association of subgingival A. actinomycetemcomitans with the morphological composition of the subgingival microbiota in a large population of patients suffering from advanced periodontitis. Subgingival plaque from the deepest pockets of every quadrant of their dentitions was sampled and pooled in 70 patients between 14 and 63 years of age, and analysed morphologically by phase-contrast microscopy. A minimum % similarity index was employed to define 4 clusters with different morphological composition of the floras. The actual proportion of A. actinomycetemcomitans was determined at 2 sites with deep periodontal pockets. All clusters harboured patients infected with A. actinomycetemcomitans. If present, in clusters predominated by motile rods or medium-sized spirochetes, the organism was found in rather low proportions (median 5.3% and 3.4%, respectively). However, the cluster with a pooled flora mainly consisting of coccoid cells revealed periodontal sites with A. actinomycetemcomitans in proportions of more than 53% (median), if the organism was present (p less than 0.01). We found a positive correlation between proportions of A. actinomycetemcomitans and cocci (R = 0.65) and negative correlations with spirochetes and motile rods (R = 0.61, R = -0.59, respectively). Cautious interpretation of subgingival plaque predominated by coccoid cells is recommended, if deep periodontal pockets and obvious signs of inflammation are present, since these pockets were found to be often infected with large numbers of A. actinomycetemcomitans.

Actinobacillus↗
PubMed1990

[The effects of orthodontic treatment on periodontal findings in adults].

After periodontal therapy 12 patients with advanced alveolar bone loss were orthodontically treated and recalled for approximately 51.1 months. Orthodontic tooth movement resulted in a mean overjet reduction of 1.7 mm, a mean overbite reduction of 1.3 mm and closure of the anterior spaces. No significant differences were observed in the probing depths and attachment levels between the test and control teeth. 31% of the treated teeth showed signs of root resorption. The results indicate that orthodontic therapy in patients with successfully treated advanced periodontal disease does not lead to further attachment loss if the patients are periodontally well maintained.

Adult↗
PubMed1990

[Quantifying bacteriological findings in the subgingival flora].

The variation of selected bacteriological parameters was investigated in 25 untreated A. actinomycetemcomitans-associated periodontitis patients. Samples of subgingival plaque were collected twice within 6 weeks with paper points for cultivation as well as with curettes for analyses of the morphological composition. Total counts of colony forming units, counts of A. actinomycetemcomitans as well as proportions of spirochetes were determined. Our results suggest a high reproducibility of quantitative data if a standardised sampling procedure with paper points is employed. On the other hand, considerable differences were observed in the morphological composition of subgingival plaque.

Actinobacillus↗
PubMed1990

[Cyclosporine-induced gingival hyperplasia in renal transplant patients].

Cyclosporine-induced gingival hyperplasia was investigated in a clinical study of 80 renal transplant patients. 34% of the patients exhibited at least mild gingival hyperplasia in the anterior, and 9% presented this finding also in the posterior region. No direct correlation was found between the oral dose or the whole blood concentration of cyclosporine and the presence of gingival overgrowth. Young and female patients were at a significantly greater risk of developing cyclosporine-induced gingival hyperplasia than the other patients. The presence of dental plaque or gingival inflammation was not found to be related to the incidence of gingival hyperplasia.

Adolescent↗
PubMed1990

[Concentrations of A. actinomycetemcomitans in subgingival plaque following short-term minocycline therapy].

In the present study we examined alterations in the proportions of cultivable flora of A. actinomycetemcomitans (A.a.) in subgingival plaque of 25 patients with different forms of periodontitis, 7 days after systemic administration of 200 mg/d Minocycline-HCl. Remarkable shifts in the flora were apparent. Sites with low or extremely low initial levels of A.a. (less than 10%) showed an increase in mean proportions from 2.7% to 15.7% (p less than 0.01). A.a. was eliminated in only 18% of sites after 1 week of minocycline therapy. Subgingival scaling resulted in 80% of sites without detectable A.a. However, if present, the organism accounted for 51% of the cultivable microflora, on average. Our data emphasize the importance of a sufficiently extended period of antibiotic therapy and efficient mechanical debridement of all tooth surfaces to eliminate the organism.

Actinobacillus↗
PubMed1989

Actinobacillus actinomycetemcomitans contamination of toothbrushes from patients harbouring the organism.

The main ecological niche of Actinobacillus actinomycetemcomitans (A.a.) seems to be the periodontal pocket, but it can also be isolated from supragingival plaque, buccal and tongue mucosa, or saliva. We examined toothbrushes from 21 patients, all identified as harbouring moderate to large numbers of A.a. in subgingival plaque, for contamination with this organism. 29% of the toothbrushes presented by our patients yielded detectable numbers of A.a. Immediately after toothbrushing this figure rose to 62%, but dropped to 50% after 1 h. Numbers of isolated A.a. on toothbrushes were weakly correlated with the degree of periodontal destruction, and significantly more numbers of A.a. on toothbrushes could be detected if the organism was found on mucous membranes or in saliva. There was no association with gingival inflammation, supragingival plaque nor mean numbers of isolated subgingival A.a.

Actinobacillus↗
PubMed1989

Phenotypic dynamics of macrophage subpopulations during human experimental gingivitis.

The purpose of the present study was to investigate whether functionally different macrophages are present in clinically healthy gingiva and during human experimental gingivitis. Eight male probands were introduced to an oral hygiene program until all reached mean Plaque and Gingival Index scores approaching zero. During the following 19 days all oral hygiene was abandoned. At d -14, 0, 2, 4, 7, 11 and 19 clinical indices and gingival biopsies were taken. Cryostat sections were incubated with monoclonal antibodies against mature macrophages (25F9), inflammatory macrophages (27E10) and anti-inflammatory macrophages (RM 3/1). Positive cells were counted in the inflammatory infiltrate (IF) and the connective tissue (CT). At d -14 elevated numbers of 27E10-positive cells were observed which decreased significantly at d 0 (p less than 0.018) and increased again at d 19 (p less than 0.026). Significant differences in the number of RM 3/1-positive cells were found between d 0 and d -14, 2, 4 and 7 (p less than 0.05) while no differences in the number of 25F9-positive cells were observed throughout this study. It was concluded that experimental gingival inflammation is characterized by the appearance and disappearance of functionally different macrophage subpopulations.

Adult↗
PubMed1989

Prevalence of alcohol related admissions to general medical units.

The prevalence of alcohol related admissions to hospitals in Manitoba was examined in an investigation limited to the general medicine units of four representative hospitals within the province. All admissions during one-month periods were closely examined. In order to determine if an admission was related to alcohol, the investigators obtained permission from each patient to ask the attending physician to rate the degree to which they felt the admission was alcohol related, and intensively reviewed all charts. The percentage of alcohol related admissions was found to range from 6.38 percent to 14.93 percent on medical units. The disorders which alcohol related patients presented on admission tended to differ from those in the not-related category. At all hospitals, disorders of the gastrointestinal system were in the majority for the alcohol related group, while the not-related group presented more difficulty in the area of cardiovascular disorders. The cost which is associated with these alcohol related admissions is large, the highest being $61,050.00 for a one-month period in one of the hospitals surveyed. The findings clearly indicate that in Manitoba, health care facilities and health care professionals are very much involved in problems related to alcohol. The findings of this study indicate that the problem is with us and we should make every effort to ensure proper and effective care, and to establish systems for the identification and referral of alcohol related admissions.

Adult↗
PubMed1989

[Vital staining and phase contrast microscopy results of plaque smears].

The information about the vitality of the plaque is limited. Only motile bacteria can be distinguished as alive. Vital staining allows to differentiate between living nonmotile rods and dead, previously motile rods. With increasing severity of the periodontal destruction, the proportion of alive respectively motile rods and vibrions increases. The vital staining shows very clearly the relation between destruction and living rods.

Adolescent↗
PubMed1989

[Efficacy of a PVP-iodine compound on selected pathogens of the oral cavity in vitro].

The study was designed to test the efficacy of a PVP-iodine antiseptic agent on potentially pathogenic oral bacteria. A 0.5% concentration of the test compound was sufficient to inhibit the growth of 7 out of 8 tested strains. Only bacteroides gingivalis continued to grow during exposure to a 0.5 and 1% concentration of the test compound. Quantitative suspension experiments demonstrated that the onset of action occurred within five minutes. Quantitative suspension experiments with protein loading resulted in a lower efficacy of the tested agent. Results indicate that the tested compound may be recommended as an oral disinfectant.

Bacteria↗