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Biomedical subjects

D E Lee

Publications and source records attributed to D E Lee.

16 recordsLinked to original sources
PubMed1991

Effects of hyperlipemia on radioimmunoassays for progesterone, testosterone, thyroxine, and cortisol in serum and plasma samples from dogs.

Hyperlipemic serum and plasma samples often are received by clinical laboratories for endocrinologic analysis by radioimmunoassay. We designed a study to determine what effect, if any, hyperlipemia has on estimation of lipid-soluble hormone concentrations determined by solid-phase radioimmunoassays. Progesterone, testosterone, thyroxine, and cortisol concentrations were determined in canine plasma and serum with various degrees of lipemia. Samples of serum, heparinized plasma, and EDTA-treated plasma were obtained from blood collected from 4 female and 4 male Beagles by use of evacuated tubes. To induce hyperlipemia in vitro, IV fat emulsion was diluted in deionized water to produce 0 (water only), 33, 67, or 100% mixtures. Twenty microliters of each mixture then was added to the subsamples of serum and plasma from each dog. Hormone concentrations were determined, using validated radioimmunoassays. Triglyceride concentrations were determined by enzymatic assay. Addition of IV fat emulsion in vitro was an accurate and reproducible means of altering triglyceride concentrations in the samples. Triglyceride concentrations as high as 700 mg/dl had no effect on radioimmunoassays for progesterone, testosterone, and thyroxine in serum, heparinized plasma, or EDTA-treated plasma. Addition of 100% (but not 33 or 67%) fat emulsion reduced the mean cortisol concentration in heparinized plasma by 12% (P less than 0.05). This severe hyperlipemia did not affect quantification of cortisol in serum or EDTA-treated plasma.

Animals↗
PubMed1991

Using data describing physician inpatient practice patterns: issues and opportunities.

Information on physicians' inpatient practice patterns is becoming increasingly available through state data systems. This article describes the kind of information that is becoming publicly available, provides illustrations of how these data can be used, and discusses issues and potential problems that may occur in using the data.

Health Services Research↗
PubMed1989

Nonconstrained total elbow arthroplasty. Development and results in patients with functional class IV rheumatoid arthritis.

Twenty-four nonconstrained total elbow arthroplasties (TEAs) in 20 patients with severe disabling functional class IV rheumatoid arthritis were reviewed retrospectively to determine the effects of their disease on the end result. The mean follow-up period was 6 years (range, 2-11 years). All planes of elbow motion improved after surgery (P less than .005), there was a highly significant reduction in pain and improvement in function (both P less than .005) and patient satisfaction was high. The severity of the disease does not appear to affect the end result when a nonconstrained TEA is used, and the results compare favorably with those of other series reported.

Adult↗
PubMed1988

Research note: reference others and self-destruction.

48 students were presented hypothetical situations having to do with smoking, drinking liquor, using other drugs, and over-eating. They were asked to indicate their reference others and the others' expectations for their behavior in these situations. Family members and friends were the most frequently named reference others, varying in importance with the situation. Perception of reference others' expectations tends to correspond with respondents' behavior, encouraging drinking and discouraging smoking and drugs. Inconsistencies between reference-others' expectations and respondents' behaviors and respondents' values elicited comments suggesting that the questions alone may lead respondents to feel dissatisfied with the behavior.

Adult↗
PubMed1987

The self-deception of the self-destructive.

317 college students as respondents were measured for suicide proneness and self-destructive behaviors and were also asked questions about self-attitudes, value for life, beliefs about suicide and self-destruction, religiosity and dogmatism. Those who score high on suicide proneness and self-destructiveness do not tend to be the same people, and they differ from one another. Correlations and factor analyses suggest the Suicide Prone are aware of their tendencies and are influenced by their value for life and beliefs about suicide and self-destruction. The Self-destructive are tied to negative self-evaluations, are less aware of their self-destructive tendencies, and score significantly higher than the Suicide Prone on dogmatism. Belief structure of the highly dogmatic person may allow those who are self-destructive to deny negative self-attitudes and to be unaware of self-destructive behaviors which are inconsistent with their beliefs.

Adult↗
PubMed1986

'Catatonia' produced by alfentanil is reversed by methylnaloxonium microinjections into the brain.

Alfentanil, a short-acting and powerful analgesic, when injected peripherally to rats (0.5 mg/kg) produced a catatonic state characterized by a rigid akinesia. The present study was designed to explore the neuroanatomical location of the opiate receptors mediating the alfentanil induced catatonia. The catatonic effect of alfentanil was measured using a bar test and depression of locomotor activity in rats tested in photocell cages during an active nocturnal phase of their cycle. Methylnaloxonium HCl (MN), a quaternary derivative of naloxone which does not readily cross the blood-brain barrier, injected into the lateral ventricle significantly reduced the catatonia at doses of 0.125-2.0 micrograms as measured in both the locomotor and bar test. MN perfusion of similar doses directly into the nucleus raphe pontis, but not in the caudate nucleus significantly antagonized the catatonia. These data complement results on alfentanil-induced muscular rigidity (Blasco et al., see companion paper) where EMG indices of rigidity in rats were reversed by microinjections of low doses of MN (0.125 and 0.5 microgram) in the nucleus raphe pontis, but not the caudate nucleus even at a high dose (4.0 micrograms). Together these results suggest that the region of the nucleus raphe pontis is an important neural substrate for opiate-induced muscular rigidity, and that the catatonic state produced by opiates depends on more diffuse opiate receptor activation of which one important component may be the nucleus raphe pontis.

Alfentanil↗
PubMed1986

Monosaccharide transport by Eimeria tenella sporozoites.

Eimeria tenella sporozoites were incubated in the presence of 3 different [14C]-labeled sugars; D-glucose, 2-deoxy-D-glucose and 3-O-methyl-D-glucose. The initial velocity, Vi, of uptake of D-glucose and 2-deoxy-D-glucose was similar, 41 micrograms/10(10) sporozoites/min and 46 micrograms/10(10) sporozoites/min, respectively; whereas that for 3-O-methyl-D-glucose was significantly lower, 17 micrograms/10(10) sporozoites/min. Initial velocity studies also revealed that glucose uptake was a saturable event, with an apparent KT of 20 mM and an apparent Vmax of 312 micrograms/10(10) sporozoites/min. Uptake was unaffected by exogenous sodium levels or the presence of ouabain. However, 0.1 mM phloretin significantly inhibited glucose uptake. Thus, it would appear that E. tenella sporozoites possess a Na-independent, phloretin-sensitive, carrier-mediated monosaccharide-transport system.

Animals↗
PubMed1985

Alternative self-destruction.

Data from 63 respondents indicate significant relationships between both suicide proneness and self-destructive behavior and reverence for life but not with self-worth. Need for research includes construction of a different measure of self-worth and creation of typologies to compare correlates of suicide-proneness and of self-destruction.

Humans↗
PubMed1985

Refractory distal ulcerative colitis responsive to 5-aminosalicylate enemas.

Patients with distal ulcerative colitis may be refractory to standard drug therapy. 5-Aminosalicylate, the active moiety of sulfasalazine, is effective topically in new onset distal colitis. Six patients with distal colitis unresponsive to sulfasalazine and corticosteroids had a prompt and dramatic response to the administration of 5-aminosalicylate enemas with clinical, sigmoidoscopic, and histological improvement. Topical 5-aminosalicylate should be a useful addition to the current available therapies for some patients with refractory distal ulcerative colitis.

Administration, Oral↗
PubMed1978

Intake of nickel and vanadium by humans. A survey of selected diets.

The nickel and vanadium contents of nine institutional diets were determined by atomic absorption spectrometry with background correction. The following values were obtained for nickel: mean concentration, 0.27 +/- 0.02 microgram/g (dry weight); range, 0.19 and 0.41 microgram/g; mean intake, 165 +/- 11 microgram/day or 75 +/- 10 microgram/1000 cal. The respective values for vanadium were: 0.032 +/- 0.004 microgram/g (dry weight); 0.019 to 0.050 microgram/g; 20.4 +/- 2.3 microgram/day or 8.9 +/- 1.0 microgram/1000 cal. Thus, vanadium is present at approximately one order of magnitude less than nickel.

Diet Surveys↗
PubMed1976

A method for qualitative identification of sugars and semiquantitative determination of lactose content suitable for a variety of foods.

A semiquantitative chromatographic technique was developed and standardized using water/chloroform extracts of foods which are spotted onto heat-activated silica gel thin layer chromatographic (TLC) plates, run in butanol/acetic acid/ether/water, developed in acids anisaldehyde, and quantitated planimetrically using Purdy and Truter's formula. Clinically significant amounts of lactose were found in low-calorie sweeteners, breads, yogurt, margarine, penicillin, Gantrisin, and other commonly ingested nondairy substances.

Beverages↗
PubMed1976

Self-destructive consequences of sex-role socialization.

Suicide proneness and self-destruction scales, together with measures of self-favorability and self-confidence, were administered to two samples (n = 106 and n = 213). The affective responses of the second sample (134 women and 79 men) were measured by asking respondents to circle adjectives classified for activity-passivity, positive-negativeness, and extroversion-introversion that expressed their feelings toward eight situations. Women scored lower than men on suicide proneness, higher on self-destruction, and lower on self-favorability and self-confidence. Women reacted more passively to the situations than men and more negatively to the public challenge situation. Women tend to extrovert (turn outward on others) their positive feelings in pleasant situations and to introvert (turn blame on themselves) their negative reactions to unpleasant situations. Men do the reverse. It is suggested that women are self-destructive in passive ways, reflecting their lack of self-favorability and confidence and manifested by failure to react to everyday situations in ego-building ways.

Attitude↗