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D F Hidvegi

Publications and source records attributed to D F Hidvegi.

12 recordsLinked to original sources
PubMed1992

The efficacy of image-guided stereotactic brain biopsy in neurologically symptomatic acquired immunodeficiency syndrome patients.

A prospective series of 50 neurologically symptomatic human immunodeficiency infected patients with intracranial lesions who underwent image-guided stereotactic brain biopsy is presented. Patients were diagnosed with primary central nervous system lymphoma (14 patients), progressive multifocal leukoencephalopathy (14 patients), toxoplasmosis (13 patients), human immunodeficiency virus encephalitis (3 patients), infarction (2 patients), and 1 patient each with metastatic adenocarcinoma, metastatic melanoma, cryptococcoma, and atypical mycobacterial infection. Two of the patients with toxoplasmosis had a second intracranial abnormality. Two biopsies resulted in either descriptive diagnosis only or were nondiagnostic; the definitive diagnostic efficacy of image-guided stereotactic biopsy was thus 96%. No deaths were incurred as a result of biopsy. Four intraoperative or postoperative hemorrhages occurred; in only 1 patient was there a residual neurological deficit related to the surgery. Image-guided stereotactic biopsy may thus be considered both safe and effective in this patient population.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Comparison of the Papanicolaou and Feulgen staining methods for DNA quantification by image analysis.

The possibility of using archival cytology material to study the evolution of neoplastic disease with regard to DNA content abnormalities was investigated. The accuracy of measuring the integrity optical density (OD) of nuclei that correlates to DNA amounts of those nuclei, on slides stained by the Papanicolaou method, was assessed and compared with a standard Feulgen method. Our data on rat liver nuclei peritoneal washings from patients with ovarian cystadenofibromas and ovarian cystadenocarcinomas suggested that analysis of cytological material using the Papanicolaou method is not reliable and that destaining the slides followed by Feulgen staining provides an optimal and reliable method of DNA quantification.

Adenofibroma↗
PubMed1989

Epithelioid hemangioendothelioma: cytomorphology and histological features of a case.

A case of epithelioid hemangioendothelioma involving the left lung, the 10th thoracic vertebra, and paravertebral soft tissues is presented. Preoperative sputum cytology was thought to show adenocarcinoma. Histologic examination demonstrated an intravascular bronchioloalveolar tumor; immunohistochemical staining for factor VIII-related antigen revealed strongly positive tumor cells. The cytomorphology of this rare neoplasm is discussed.

Aged↗
PubMed1988

Pleomorphic carcinoma of the pancreas with osteoclast-like giant cells expressing an epithelial-associated antigen detected by monoclonal antibody 44-3A6.

This article describes a case of pleomorphic adenocarcinoma of the pancreas, metastatic to the adrenal glands and liver, that has unique cytological and histological features. Cytology demonstrated numerous mononuclear and multinucleated tumor giant cells as well as numerous osteoclast-like giant cells. These features were also seen in the histological sections, as were numerous clear cells. Electron microscopy demonstrated features suggesting an epithelial origin of the epulis cells. Prominent immunoreactivity with the monoclonal antibody (MCA) 44-3A6 of these cellular constituents was seen in the aspirate and in the tissue sections. These data provide further evidence to support the epithelial origin of these cellular components.

Adenocarcinoma↗
PubMed1985

Nasopharyngeal carcinoma: antikeratin immunohistochemistry and electron microscopy.

Eighteen examples of nasopharyngeal carcinoma (NPC), a tumor with potential diagnostic difficulty, were studied retrospectively. Using the WHO classification, 16 cases were undifferentiated carcinoma (UC). Immunohistochemistry for each tumor was performed on paraffin sections using two commercially available polyclonal antisera and a monoclonal antibody, AE-1. Method 1 used trypsinization, overnight incubation with the primary antibody and the avidin-biotin complex (ABC) technic. Method 2 used a 20-minute incubation with the primary antibody without trypsinization and employed the peroxidase-antiperoxidase (PAP) technic. Method 2 is the one most frequently employed by pathologists who use immunohistochemistry as a diagnostic aid. Method 1 gave clear positive results in each case with antibody AE-1 and, in most cases, with the polyclonal antisera. Electron microscopy in 10 cases demonstrated desmosomes in each case and easily demonstrable tonofilaments in five. The results of this study indicate that in the diagnosis of UC, the most common variant of NPC, squamous differentiation can be documented readily by electron microscopy and immunohistochemistry for keratin proteins. With the latter, optimization of technic is essential for reliable results.

Adult↗
PubMed1977

Olfactory neuroblastoma: an ultrastructural study.

The ultrastructural features of two olfactory neuroblastomas are reported. By means of light microscopy, one was well differentiated while the other was a poorly differentiated, small cell neoplasm. The latter case required ultrastructural examination to establish the diagnosis. Electron microscopy of human tumors for diagnostic purposes may be particularly helpful in deciphering small, round cell tumors. Catecholamines were not biochemically detected in a portion of recurrent tumor from Case 2. The significance of this is equivocal in view of the limited previous biochemical studies of this neoplasm. However, by both light and electron microscopy, the morphology of olfactory and sympathetic neuroblastomas are strikingly similar.

Adolescent↗
PubMedDate not supplied

Improved transmission electron microscopy technique for the study of cytologic material.

A modification of the technique of Coleman et al for the preparation of single cells in cytologic specimens for electron microscopy (EM) is described. By employing materials in the initial cytologic processing that are useful for EM, such as a paraformaldehyde-glutaraldehyde fixative, lactated Ringer's solution as a rinsing medium, glycerol as a mounting medium and cacodylate buffer for removal of coverslips, the use of alcohol fixatives and standard mounting media could be avoided. This preserved the cytoplasmic detail, which is usually degenerated in cells removed from cytologic specimens and processed for EM.

Biopsy, Needle↗
PubMedDate not supplied

Cytomorphologic and cytochemical aspects of malignant histiocytosis with spindle-cell differentiation. A case report.

Spindle-cell differentiation and tumor formation have been observed rarely in cases of malignant histiocytosis. We describe below one case of malignant histiocytosis with spindle-cell differentiation. Touch preparation of autopsy material revealed cytologic features that correlated with the histologic appearance of atypical components of this entity, such as spindle and nonspindle histiocytes and abundant erythrophagocytosis. Cytochemical properties indicated the histiocytic nature of the cells that composed this tumor. A morphologic account of the different types of cells is presented.

Child↗
PubMedDate not supplied

Crush preparations of lesions of the central nervous system. A useful adjunct to the frozen section.

A series of 32 cases in which crush preparations were used in addition to frozen sections for the rapid diagnosis of lesions of the central nervous system (CNS) is presented. The cytopathologic features in crush preparations of astrocytomas, glioblastomas multiforme and a pituitary adenoma are described. Excellent preservation of cellular detail was seen in the crush preparations. Frozen sections lacked cytologic detail but provided a better view of the tissue architecture. The crush preparations yielded the correct diagnosis in 29 of the 32 cases. In the other three, a secondary component of the neoplasms (oligodendroglioma and fibrosarcoma) was identified only in the paraffin sections. Use of both frozen sections and crush preparations is recommended for all cases in which an immediate diagnosis of a CNS lesion is required.

Adenoma↗
PubMedDate not supplied

Clear-cell adenocarcinoma of the female urethra.

Malignant cells were found in the urine of a 54-year-old woman. The papillary tumor subsequently removed from the posterior wall of the urethra proved to be a clear-cell adenocarcinoma. The cytologic, histologic and ultrastructural features of this tumor were very similar to clear-cell adenocarcinomas arising in the female genital tract, suggesting a possible Müllerian origin of this neoplasm.

Adenocarcinoma↗