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Biomedical subjects

D F SHARMAN

Publications and source records attributed to D F SHARMAN.

17 recordsLinked to original sources
PubMed1963

Iontophoretic release of adrenaline, noradrenaline and 5-hydroxytryptamine from micropipettes.

Estimations have been made of the amounts of adrenaline, noradrenaline and 5-hydroxytryptamine released by iontophoresis from micropipettes. In most experiments, the amount released was linearly related to the total electrical charge, but the transport numbers for different pipettes, filled with samples of the same solutions of adrenaline or of noradrenaline, varied substantially. Two pipettes containing noradrenaline failed to release any appreciable amounts of the drug. The transport number of 5-hydroxytryptamine was relatively constant (mean, 0.14).

Epinephrine↗
PubMed1963

THE SUBCELLULAR LOCALIZATION OF DOPAMINE AND ACETYLCHOLINE IN THE DOG CAUDATE NUCLEUS.

The distribution of dopamine (3,4-dihydroxyphenylethylamine) between the subcellular fractions from homogenates of dog caudate nucleus was compared with the distributions of acetylcholine, 5-hydroxytryptamine and lactate dehydrogenase activity. The distributions of noradrenaline and 5-hydroxytryptamine between the subcellular fractions from homogenates of dog hypothalamus were also determined. Most of the dopamine, in contrast to acetylcholine, occurred in the soluble supernatant fraction; the remainder was associated with the fractions rich in pinched-off nerve ending particles, but localization in any one fraction was not as sharp as that of acetylcholine. Evidence is presented that suggests that the dopamine occurs in a free or easily released form throughout cell cytoplasm.

Acetylcholine↗
PubMed1962

Drug-induced changes in the concentration of 5-OR indolyl compounds in cerebrospinal fluid and caudate nucleus.

The effects of reserpine and imipramine on the concentration of indolyl compounds bearing an OR group in position 5 (5-OR indolyl compounds) in the cerebrospinal fluid and the caudate nucleus of the dog have been studied. Reserpine was shown to produce an increase in the concentration of these compounds in the cerebrospinal fluid. In the caudate nucleus reserpine produced a fall in the concentration of the basic 5-OR indolyl compounds accompanied by an equivalent increase in the concentration of acidic 5-OR indolyl compounds. No significant change in the concentration of 5-OR indolyl compounds in the cerebrospinal fluid was observed after treatment with imipramine.

Animals↗
PubMed1962

Noradrenaline content in the heart and spleen of the mouse under normal conditions and after administration of some drugs.

The noradrenaline content of the heart and spleen was investigated in normal mice and in mice treated with drugs. A modification of the methods of Bertler, Carlsson & Rosengren (1958) was used for extraction, and of v. Euler & Floding (1955) for fluorimetric estimation of the amine. In normal mice the mean noradrenaline content of the heart was 0.55 mug/g and that of the spleen 0.26 mug/g fresh tissue. Iproniazid (100 mg/kg), nicotine (0.1 mg/kg) and histamine (0.5 mg/kg), given 1 and 3 hr before killing the mice, did not significantly change the concentration of noradrenaline in the heart. Neither did nicotine and histamine, administered 1 hr before death, significantly alter the noradrenaline content of the spleen. The rapid changes in the catechol amine content of mouse tissues reported with these drugs by De Schaepdryver & Preziosi (1959) were not observed. In contrast, reserpine (2.5 mg/kg), methyl reserpate methyl ether (1 mg/kg), and methyl 18-epireserpate methyl ether (2 mg/kg) caused severe depletion of noradrenaline from the heart and spleen of the mice.

Anesthetics, General↗
PubMed1961

On the question of the occurrence and metabolism of 5-hydroxytryptamine and related indole compounds in mammalian semen.

The content of 5-hydroxytryptamine was examined in the semen of man, bull, ram, boar and dog. Spectrophotofluorimetric and chromatographic analysis of extracts prepared by several methods has shown that the semen contains only minute quantities of 5-hydroxytryptamine, if any. One cannot attribute to 5-hydroxytryptamine the uterus-stimulating effect which the human seminal plasma is known to exert. Tryptophan, although present in the seminal plasma, is not converted to 5-hydroxytryptamine on incubation of semen. 5-Hydroxytryptophan is oxidatively deaminated, but not decarboxylated, by spermatozoa. 5-Hydroxytryptamine itself, like tryptamine and tyramine, is not deaminated by either whole semen or washed suspensions of ram spermatozoa.

5-Hydroxytryptophan↗