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Biomedical subjects

D Ferre

Publications and source records attributed to D Ferre.

11 recordsLinked to original sources
PubMed1993

Discrepancies of DNA content of various solid tumours before and after culture measured by image analysis. Comparison of cytogenetical data.

Parallel cytophotometric ploidy studies and cytogenetic analysis were performed on 15 various human solid tumours. The quantification of DNA by image analysis was carried out on cytological imprints of fresh tumours and on smears obtained after cell culture. The results obtained by both sets of calculations were compared with each other and with the cytogenetic results. 6 cases (40%) showed concordance between the 3 techniques. One case was aneuploid for both DNA image analysis measurements but the cytogenetic data showed only a diploid stem line. In 3 cases out of 15 (20%), smears DNA analysis and cytogenetic results were concordant: in 2 tumours, the culture step failed to preserve aneuploid stem lines that were present in the imprint analysis. In the third one, a minority tetraploid peak observed after culture was absent on the imprint slide. Concordance between imprints and cytogenetic data and discordance with smears' analysis was observed in 3 cases (20%). These 3 cases were diploid or near diploid but the DNA analysis on the smears after culture showed an aneuploid stem line in each case. The last 2 cases showed a total disagreement between the 3 techniques. By measuring the DNA content with an image analyser, the observer can ensure that only tumoral cells are taken into account. The present study revealed that cytogenetic data represent only about 60% of the population that is effectively present in the culture dish and that the cultured population represents only 47% of the population present on the fresh tumour imprint.

Cells, Cultured↗
PubMed1992

Can chromatin texture predict structural karyotypic changes in diploid cells from thyroid cold nodules?

To assess the effect of a single chromosomal translocation on the nuclear phenotype of human cells, seven diploid adenomas and five diploid carcinomas of the thyroid gland were studied using quantitative nuclear morphometry. Four adenomas and three carcinomas were cytogenetically normal, whereas three adenomas and two carcinomas had a unique chromosomal translocation. A densitometric parameter discriminated adenomas from carcinomas (skewness of the optical density histogram, SODH), and tumours with and without chromosomal translocation (standard deviation of the optical density, SDODH). These results demonstrate that single chromosomal structural rearrangements produce quantifiable alterations of nuclear organisation, but that other nuclear features which do not express an aneuploid DNA content or an abnormal karyotype differentially characterise benign and malignant conditions.

Adenoma↗
PubMed1990

Chromosomal changes in renal cell carcinoma. No evidence for correlation with clinical stage.

A cytogenetic study was performed in 18 renal cell carcinomas using a culture method previously described. The significance of chromosome involvement in clonal aberrations was evaluated according to the mean + 1 SD objective criterion. Chromosomes 3, 7, 9, and 17 were preferentially involved in both numerical and structural changes. The cytogenetic data have been correlated with the clinical staging, but in contrast to a previous study, abnormalities of chromosome 3 were not associated with a higher clinical stage.

Adult↗
PubMed1990

Chromosomal changes in thyroid tumors. Relation with DNA content, karyotypic features, and clinical data.

A cytogenetic study was performed in 63 thyroid tumors after a monolayer short-term culture. Clonal chromosomal changes were found in 47% of carcinomas and 31% of adenomas. Chromosome 7 was altered in 40% of cytogenetically abnormal tumors. The modal DNA index measured in 26 tumors was consistent with the chromosomal mode in 88% of cases. A quantitative morphometric analysis of nuclear features differentiated between diploid thyroid adenomas with or without a single translocation, which suggests that they have different biological properties. Clonal chromosomal changes were observed in 78% of carcinomas with an aggressive behavior, but only 28% of those had no risk factors. The two patients who died early had abnormalities of chromosome 7.

Adenoma↗
PubMed1989

Frequent clonal chromosomal changes in human non-malignant tumors.

A cytogenetic analysis has been performed on 109 non-malignant human solid tumors of various histological types after short-term culture. These tumors were derived from epithelial, mesenchymal, embryonal and neurogenic tissues. The chromosome count was in the diploid range in virtually all specimens. Clonal chromosomal changes were found in 37% of tumors: 20% had numerical deviations, 12% structural rearrangements, and 5% both karyotypic alterations. Chromosome 7 was most frequently involved in 25% of abnormal specimens. Our results suggest that chromosomal changes contribute to non-malignant tumorigenesis and that their analysis may provide information about the genetic events which shift benign tumor cells to malignant behavior.

Aneuploidy↗
PubMed1989

Drug-related chromosomal changes in chemoresistant human ovarian carcinoma cells.

Four resistant sublines were derived from the sensitive human ovarian carcinoma IGROV 1 (OV1-P) cell line by exposure to increasing concentrations of vincristine, doxorubicin, and cisplatinum. The vincristine-resistant sublines expressed the MDR phenotype associated with a complete reversion of malignant properties. Cytogenetic studies of sensitive and resistant cells have been repeatedly performed over a 1-year period. Consistent and stable drug-related chromosomal structural rearrangements have been observed in each resistant population affecting chromosomes 3, 4, 6, 8, 11, 22, and X. The most significant result was the presence in OV1/P cells of a minor subclone with a del(11)(p13) marker that represented the whole OV1/VCR population. This result suggests a possible role of this deletion either in the drug-selection process, or in the malignant reversion.

Adenocarcinoma↗
PubMed1986

Recurrent deletion of the short arm of chromosome 3 in human renal cell carcinoma: shift of the c-raf 1 locus.

A cytogenetic study performed on 6 human renal cell carcinomas after short-term culture on extracellular matrix with serum-free medium showed aneuploidy without structural changes in 2 tumors and a rearrangement of the short arm of chromosome 3 in 4 tumors, including deletions and a translocation involving the 3p14 and 3p21 bands. Chromosomal in situ hybridization with a c-raf 1 probe demonstrated that in 2 renal cancers with del3(p14 or 21) the cellular oncogene had shifted from 3p25 to 3p14 as a result of an interstitial deletion.

Carcinoma, Renal Cell↗
PubMed1985

[Antispermatozoa autoantibodies in male sterility. Value of the detection of surface immunoglobulins G by the MAR test].

The mixed antiglobulin reaction (MAR) test for immunoglobulins G was performed on 574 spermograms, resulting in the detection of surface auto-antibodies directed against spermatozoa in 4.18% of the cases. Concomitant investigations of spermograms for spontaneous agglutination and cross-penetration in a control cervical mucus, together with assays of antispermatozoa antibodies in serum and seminal fluid, have led to the diagnosis of asthenospermia in 87.5% of the cases. Spontaneous sperm agglutination was present in all cases; the cross-penetration test was negative or doubtful in 92.3%. Auto-antibodies against spermatozoa were found in 76.2% of serum and/or seminal fluid samples.

Autoantibodies↗
PubMedDate not supplied

Identification of a clustering of chromosomal breakpoints in the analysis of 203 human primary solid tumor non specific karyotypic rearrangements.

The distribution of 271 chromosomal breakpoints involved in 203 clonal structural non "specific" rearrangements identified in 82 human primary solid tumors of various histologies has been analyzed. According to the "mean + 1 SD" criterion the normalized breakpoint frequency was significantly higher for chromosomes 1p, 3p, 3q, 4q, 6q, 7q, and 11p. Clusters of 3 or more breakpoints were assigned to only 24 of the 329 bands (7%) of the standard karyotype, and 9 high density breakpoint segments have been identified. These results indicate that a nonrandom pattern of chromosomal rearrangements can be extracted from the complex karyotypic changes observed in solid tumors, which suggest that the involved regions play a general role in tumorigenesis.

Chromosome Aberrations↗
PubMedDate not supplied

Cytogenetic changes in 67 cranial and spinal meningiomas: relation to histopathological and clinical pattern.

The cytogenetic analysis of 67 meningiomas (58 intracranial and 9 spinal tumors) identified chromosomal abnormalities in 63% of cases. When chromosomes involved in numerical and structural changes with a frequency of more than one standard deviation above the mean were considered, distinct cytogenetic patterns could be identified according to sex, anatomical location and histology. The chromosomes more frequently affected were 1, 2, 3, 4, 8, 14, 15, 19, 22, Y. No conclusion could be drawn regarding the prognostic significance of these karyotypic alterations.

Adult↗