Biomedical subjects

D Filipović

Publications and source records attributed to D Filipović.

At least 19 recordsLinked to original sources

PubMed1993

Peritubular Na-K exchange ion pump in maleate-treated frog kidney proximal tubular cells.

1. After perfusion of isolated frog kidneys for 1 hr with 10(-3) or 10(-2) M maleate Ringer, the peritubular membrane potential gradually declined in a dose-dependent manner. 2. The ouabain-like effects of maleate on cell Na and K activities were dose-dependent and smaller than the effects of zero K or 10(-4) M ouabain. Intracellular pH was not altered in the presence of 10(-2) M maleate. 3. The driving force for Na entry into the cell was reduced, respectively, to 81.4 and 58.4% (of control) in the presence of 10(-3) and 10(-2) M maleate. 4. There was no histochemically detectable inhibition of proximal tubule Na-K ATPase activity during 3 hr of perfusion with 10(-2) M maleate.

Animals↗

PubMed1993

[Habituation and effects of protein synthesis blockers on neuronal activity in the cerebral sensorimotor cortex in rabbits].

The investigation of brain functions has been for many years an exciting and at the same time a very complex and enigmatic domain of research. In our experiments we followed the occurrence of habituation, a simple form of learning, during a peripheral light stimulation, in sensorimotor cortex neurons of rabbits. In the majority of the mature neurons habituation occurred with repeated light stimulations with same features, while in immature rabbits it was less frequently observed and was slower. Since in basic patterns of habituation and learning, cell protein plays a significant role, we followed the effects of protein synthesis blockers in the process of training and electrogenesis. Microiontophoretic application of actinomycin D and cyclohexamide compromised the occurrence of habituation in high number of registered neurons, while the application of a neurotransmitter, acetyl choline, promoted habituation in the sensorimotor brain cortex neurons of immature experimental animals.

Acetylcholine↗

PubMed1993

[The effect of homocarnosine on neuronal activity during satiation and starvation].

We investigated effects of homocarnosine (gamma-aminobutyric-histidine) dipeptid on neuron nucleus caudatus and hypocampus activity in small and hungry experimental animals--awake grown-up rabbits. In the given conditions, through glass microelectrodes having several channels, an extracellular registration of impulse discharge was performed as well as the microapplication of homocarnosine (HC) in the immediate surroundings of registered neurons. The obtained results show that the investigated different functional conditions of the organism are manifested in the neurons' activity of the investigated brain structures. Homocarnosine represses the activity of most registered neurons in both satiated and hungry experimental animals, while that effect is more manifested in satiated animals. The demonstrated effects of homocarnosine point to its probable neuromediator role.

Action Potentials↗

PubMed1992

[The effect of clonazepam on experimental epilepsy induced by electrostimulation].

The investigations were aimed at the study of effects of preparations which belong to the benzodiazepine group-Clonazepam (CZ), on total bioelectric activity (EEG) and epileptic discharge induced by electrostimulation of the hippocampus in the experimental animals (awake adult rabbits). Clonazepam was administered both intramuscularly (i.m.) and intravenously (i.v.) at a dose of 0.2-2.5 mg/kg of the body mass. The obtained results show that the administration of clonazepam prolongs the duration and the arrangement of the spindle in the bioelectric activity in rabbits and stops epileptic attacks of the grand mal type.

Animals↗

PubMed1985