Requirement for transcription in the neighborhood of the phage attachment region for lysogenization of Escherichia coli by bacteriophage lambda.
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Coliphages↗
Biomedical subjects
D Freifelder
Publications and source records attributed to D Freifelder.
Zonal centrifugation.
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Centrifugation, Zonal↗
Studies on lysogeny in Escherichia coli with bacteriophage lambda. Physical observation of the insertion process.
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Bacterial Proteins↗
The termini of bacteriophage T7 DNA.
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Adenine↗
Stimulation of nuclease activity by thymine starvation.
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Adenine Nucleotides↗
X-ray inactivation of bacteriophages: role of O 2 -dependent damage in single- and double-strand DNA phages.
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Air↗
Electron microscopic study of the ethidium bromide-DNA complex.
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Bromides↗
Persistence of small fragments of newly synthesized DNA in bacteria following thymidine starvation.
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Coliphages↗
Apparatus for preparation of linear density gradients.
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Centrifugation, Zonal↗
A phage lambda endonuclease controlled by genes O and P.
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Centrifugation, Density Gradient↗
Interruption of DNA injection by x-irradiation of phage lambda.
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Adsorption↗
Lack of polarity of DNA injection by Escherichia coli phage lambda.
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Carbon Isotopes↗
The formation of homoimmune double lysogens of phage lambda and the segregation of single lysogens from them.
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Centrifugation, Density Gradient↗
Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.
We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The causes of the culture-to-culture variation in recovery of covalent circular deoxyribonucleic acid were investigated but an understanding of this is not yet at hand. Some commonly used conditions which drastically reduce the recovery of covalent circles are described.
Bacteriolysis↗
Molecular weights of coliphages and coliphage DNA. I. Measurement of the molecular weight of bacteriophage T7 by high-speed equilibrium centrifugation.
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Amino Acids↗
Molecular weights of coliphages and colip- hage DNA. II. Measurement of diffusion coefficients using optical mixing spectroscopy, and measurement of sedimentation coefficients.
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Coliphages↗
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.
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Centrifugation, Density Gradient↗
Topological relationship of prophage lambda to the bacterial chromosome in lysogenic cells.
X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying lambda prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of covalent circular DNA from the product show that the prophage DNA is linearly inserted into the sex factor, as predicted by Campbell's model for lysogenization.
Centrifugation, Density Gradient↗