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D Fumarola

Publications and source records attributed to D Fumarola.

At least 199 records · Page 11Linked to original sources
PubMedDate not supplied

Limulus test, parenteral drugs and biological products: an approach.

The Authors report the results concerning the control of presence of bacterial endotoxins in parenteral drugs, blood products and immunological materials, by means of Limulus amebocyte lysate test. All the data suggest that Limulus assay is a simple, rapid, specific and sensitive test for endotoxin detection, mostly when it is correctly performed.

Antibodies, Bacterial↗
PubMedDate not supplied

[Possible contamination of preparation of transfer factor with bacterial endotoxins].

In order to detect the possible contamination with bacterial endotoxins of some preparations of Transfer Factor, Limulus amebocyte lysate test was performed. The results obtained (TF preparations positive with L.T. for endotoxin assay) call sur detection to evaluate very carefully the immunologic responses in vitro ad in vivo, with these biological products contaminate can induce. In fact, bacterial endotoxins with their multiple potent effect on biologic system, represent a contant possible surce of variability in experimental biology and pathology.

Drug Contamination↗
PubMedDate not supplied

Inhibition of ADP-induced platelet aggregation as a possible test for evaluation of the enterotoxigenicity of some enterobacteria. Preliminary study.

Some culture filtrates or enterotoxin preparations from enterobacteria that activate the adenylate cyclase system (vibrio cholerae, LT fraction from escherichia coli and klebsiella pneumoniae, shigella dysenteriae type 1) exibit an inhibiting effect on ADP-induced platelet aggregation, while other enterotoxin preparations not effective on adenylate cyclase system, don't interfere with this model. The A. propose the platelet aggregation as cellular assay to detect enterotoxin fractions effective upon adenylate cyclase system.

Adenosine Diphosphate↗
PubMedDate not supplied

[Verification of contamination with bacterial endotoxins in blood derivatives used in coagulative disorders: observations with the Limulus test].

The AA. expose the results of an investigation for an in vitro endotoxin assay by Limulus lysate test in blood products employed in coagulative area. The Limulus assay has been shown to be a simple, rapid, accurate and sensitive method of detecting bacterial endotoxin or endotoxin-like material in these biological products.

Biological Products↗
PubMedDate not supplied

[Lipopolysaccharides of Rickettsiaceae and the Limulus endotoxin assay].

The A.A. have examined by Limulus endotoxin assay some LPS preparations from R. typhi, R. slovaka, C. burnetti phase I and II, as a demonstration of endotoxicity. All the preparations tested were able to gel the Limulus amoebocyte lysates, even if to a different degree of reactivity. The results add credibility to the hypothesis that LPS from Rickettsiaceae can represent at least in part the pathogenetic mediators of some manifestations described in Rickettsiosis; however, without endotoxin assay in the blood of patients, one cannot assume to this be true.

Coxiella↗
PubMedDate not supplied

[Attempted protection against experimental endotoxic shock].

Protection against endotoxin challenge. The AA. have summarized shortly some experimental data dealing with trials of protection against endotoxin challenge pointing out, mostly, the effects of phosphate-containing compounds, as well as dextrane, anticoagulants, platelets, polymyxin B, and serum antiendotoxin and Lipid A antiserum. They have reported some experiments carried out by means of Limulus amebocyte lysate.

Animals↗
PubMedDate not supplied

[Induction of thromboplastin-like procoagulating activity by Mycoplasma pneumonia].

The capacity of a strain of Mycoplasma pneumoniae to induce the production of procoagulant activity by human mononuclear cells is described. This finding could contribute to the understanding of the pathogenetic mechanism(s) involved in the thromboembolic complications often associated with fatal cases of Mycoplasma pneumoniae infection.

Blood Coagulation↗
PubMedDate not supplied

[Exerimental study of enterotoxic activity in some strains of Salmonella typhimurium: observations on the model of ADP-induced platelet aggregation].

In a previous report it has been shown that enterotoxin preparations (cholera and E. Coli) able to activate adenylatecyclase system, abrogated platelet aggregation induced by ADP. In the present study the same experimental model has been applied to some purified filtrates from Salmonella typhimurium strains. Preparations from 986 and TLM (invasive strains causing also fluid secretion) interfere with platelet aggregation, while 1027 strain (invasive but not evoking fluid secretion) didn't show such effect. It has been argued, as Sandefur and Peterson have demonstrated with different experimental models, that the system of platelet aggregation induced by ADP is valid for some strains of Salmonella typhimurium, which act with a mechanism partially involving adenylatecyclase system (i.g. cholera-like).

Adenosine Diphosphate↗
PubMedDate not supplied

[Role of possible endotoxin contaminants present in some preparations of biological products and injectable drugs].

On the basis of personal contributions and of most recent data, the A. reports some observations and comments regarding the role of possible contaminating endotoxins demonstrated in some biological products and parenteral solutions used in clinical and experimental studies. Since endotoxins even in minute amounts have a variety of biological effects, the use of such contaminated preparations can lead to spurious results and erroneous conclusions. The Limulus assay appears to be a rapid, specific and sensitive method for detection and quantitation of endotoxin in various biological and pharmaceuticals products before they are used in clinical and experimental investigations.

Biological Products↗
PubMedDate not supplied

[Generation of pro-coagulation activity by leukocytes stimulated in vitro with lipoglycans from mycoplasmas].

Lipoglycans represent a special type of lipopoly-saccharide that differs in structure from the well-known gram-negative bacteria lipopolysaccharide. After briefly describing their most important characteristics, the authors take into consideration the in vitro interaction between lipoglycans from Acholeplasma granularum, Acholeplasma oculi, and Acholeplasma axanthum and human leukocytes in terms of production of procoagulant activity. The results obtained show that the examined lipoglycans possess, similarly to lipopolysaccharides, the capacity to induce the production of procoagulant activity, thromboplastin-like, from human mononuclear cells. However, the pathophysiological significance of this endotoxin-like activity remains to be established.

Blood Coagulation Factors↗