Effect of hypophysectomy on renal function in the western painted turtle (Chrysemys picta belli).
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Biomedical subjects
Publications and source records attributed to D G Butler.
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A detailed clinical examination was conducted and blood samples were collected from a total of 151 normal calves as soon as possible after birth and at two to three day intervals until the calves were ten days old. The mean venous pH values for calves from birth to ten days of age was 7.38 +/- 0.05. The mean serum sodium, potassium, magnesium, inorganic phosphate, calcium and chloride ion concentrations in normal calves from birth to ten days of age were 148 +/- 13, 5.4 +/- 0.8, 2.1 +/- 0.4, 4.3 +/- 0.8, 5.6 +/- 0.5, 95 +/-5, mEq/litre respectively. The mean serum osmolality in normal calves from birth to ten days of age was 280 +/- 12 mOsm/litre.
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Pressor responses to [Ile5]-Angiotensin II and [Val5]-Angiotensin II were measured following single intravenous doses of 0.0075, 0.075, 0.375, 0.75 and 7.5 micrograms/kg b.w. in anesthetised rats. [Val5]-Angiotensin II was significantly (10 per cent) more potent than [Ile5]-angiotensin II when log dose was plotted against the increase in carotid arterial pressure. Doses of 0.075 microgram/kg b.w. or more of each of the two peptides gave a full pressor response within 30 seconds. However, only at the highest and, probably, nonphysiological dose of [Ile5]-Angiotensin II (7.5 micrograms/kg b.w.) was there a significant increase in noradrenaline and adrenaline concentrations measured using a radioenzymatic assay. [Val5]-Angiotensin II was more potent than [Ile5]-Angiotensin II insofar as plasma noradrenaline increased significantly (p < 0.05) following doses of 0.75 and 7.5 micrograms/kg b.w.; adrenaline at the higher dose only. Plasma dopamine was unresponsive to both peptides.