Mechanical ventilation: weaning problems and techniques.
Explore the source record for details and available documents.
Female↗
Biomedical subjects
D G Oliver
Publications and source records attributed to D G Oliver.
Detection of Salmonella dublin mammary gland infection in carrier cows, using an enzyme-linked immunosorbent assay for antibody in milk or serum.
An ELISA has been developed for measurement of milk and serum IgG concentrations directed against Salmonella dublin. Four groups of cows were studied: group A--7 experimentally challenge-exposed cows (infected, recovered group); group B--6 normal uninfected randomly selected control cows; group C--7 naturally occurring S dublin carrier cows; and group D--6 normal uninfected S dublin negative cows from the same herd as group C. Group-A cows were inoculated orally, or inoculated orally and then IV, but none became a S dublin carrier. As expected, all 7 group-A cows responded with a marked increase in ELISA titer after oral exposure to virulent S dublin, starting with a mean serum titer of 17.7% and reaching a peak mean serum titer of 79.3% approximately 76 days after initial exposure. As determined by necropsy and organ culturing of the remaining cows, none of the group-A cows became carriers. The mean serum ELISA titer for group-B uninfected control cows was 14.1% (SD +/- 12.8%). The mean milk ELISA titer was -1.0% (SD +/- 5.5%). Colostrum and then milk gave false-positive results for up to 2 weeks after onset of lactation. Group-B cows were culture negative for S dublin in feces and milk during lactation, and when tissues were cultured after euthanasia. Milk and serum samples for ELISA, and milk and fecal samples for culturing were taken from all group-A and -B cows twice a week for 6 months. Statistical correlation (P less than 0.05) was found between serum and milk ELISA titers.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus.
A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpene-lactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forced-air microplate incubator.
Alkaline Phosphatase↗
Enzyme-linked immunosorbent assay for the detection of toxoplasmosis in swine: interpreting assay results and comparing with other serologic tests.
An enzyme-linked immunosorbent assay (ELISA) was developed for the serodiagnosis of swine toxoplasmosis. The results of the ELISA were interpreted as percentage (% ELISA) and ELISA titer and were compared with results of the dye test, indirect fluorescent antibody test, and indirect hemagglutination test. The results obtained from both the % ELISA and ELISA titer directly correlated with the dye test results. The ELISA is a rapid, sensitive, and readily performed serologic test that should be ideal for examining (screening) swine for toxoplasmosis.
Animals↗
Liquid crystal thermography. A method for monitoring temperature gradients in microtitration plates.
Precise quantitative heat transfer information in microtitration plates can be obtained by filling the wells of a microtitration plate with cholesteric liquid crystals and incubating the plates at the desired temperature in different incubators. The liquid crystals indicate temperature by changes in discrete reproducible colors over various temperature ranges. With these instrumented plates, interwell thermal gradients may be documented visually and are in close agreement with results obtained by using wire thermocouple measuring techniques.
Cholesterol↗
Thermal gradients in microtitration plates. Effects on enzyme-linked immunoassay.
Temperature studies of microtitration plates demonstrate that the use of a common bacteriology incubator for heating the plates can cause a phase log of over 30 min for the fluid in the wells to reach 37 degrees C from ambient temperature, and that a temperature gradient of as much as 1.6 degree C can exist between the peripheral and center wells. This gradient is a cause of the "rim" of edge effect noted in enzyme immunoassay using microtitration plates. The problem is corrected by the use of a specially designed forced air microtitration plate incubator.
Enzyme-Linked Immunosorbent Assay↗
Diffuse glioma of the spinal cord with hypoglycorrhachia.
Explore the source record for details and available documents.
Adult↗