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Biomedical subjects

D Galvan

Publications and source records attributed to D Galvan.

6 recordsLinked to original sources
PubMed1999

Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to the structure determination of medium and large macrocycles formed by palladium(0)-catalyzed allylation of arenesulfonamides, sulfamide, and cyanamide.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allowed the direct determination of the extent of macrocyclic and linear oligomer formation in the palladium(0)-catalyzed allylation of highly acidic and non-nucleophilic arenesulfonamides, sulfamide, and cyanamide. Palladium-containing 15-membered-ring macrocyclic compounds gave unusual [M - H](+) ions besides [M + Na](+) and [M + K](+) adducts. Copyright 1999 John Wiley & Sons, Ltd.

Journal Article↗
PubMed1999

Location of the permeation pathway in the recombinant type 1 inositol 1,4,5-trisphosphate receptor.

The inositol 1,4,5-trisphosphate receptor (InsP(3)R) forms ligand-regulated intracellular Ca(2+) release channels in the endoplasmic reticulum of all mammalian cells. The InsP(3)R has been suggested to have six transmembrane regions (TMRs) near its carboxyl terminus. A TMR-deletion mutation strategy was applied to define the location of the InsP(3)R pore. Mutant InsP(3)Rs were expressed in COS-1 cells and single channel function was defined in planar lipid bilayers. Mutants having the fifth and sixth TMR (and the interceding lumenal loop), but missing all other TMRs, formed channels with permeation properties similar to wild-type channels (gCs = 284; gCa = 60 pS; P(Ca)/P(Cs) = 6.3). These mutant channels bound InsP(3), but ligand occupancy did not regulate the constitutively open pore (P(o) > 0.80). We propose that a region of 191 amino acids (including the fifth and sixth TMR, residues 2398-2589) near the COOH terminus of the protein forms the InsP(3)R pore. Further, we have produced a constitutively open InsP(3)R pore mutant that is ideal for future site-directed mutagenesis studies of the structure-function relationships that define Ca(2+) permeation through the InsP(3)R channel.

Amino Acid Sequence↗
PubMed1999

Differences in the use of American Sign Language morphology by deaf children: implications for parents and teachers.

Newport (1988) has noted differences in how American Sign Language (ASL) is used by the following three groups of deaf adults: those with deaf parents (native signers); those, with hearing parents, who learned ASL upon entering school at age 5 years (early signers); and those who learned to sign after puberty (late signers). The present study extends this research to children by investigating the use of morphological inflections in ASL by native and early signers. Thirty deaf children between ages 3 and 9 years were asked to sign a story in ASL. The videotaped stories were analyzed for morphological and contextual complexity. Qualitative differences were found between native and early signers on measures relating to the aspectual complexity of signs but not on measures relating to the complexity of the utterance. Implications of these differences are discussed in terms of communication at home and ASL use in the classroom.

Child↗
PubMed1986

[Quantitative analysis of lymphocyte and monocyte levels in patients after operations for breast cancer].

The much less than Quantigen T and B Cell Assay much greater than method is used to evaluate T and B lymphocyte levels, Nuls Cells and monocytes in the peripheral venous blood of patients surgically treated for breast cancer. The results obtained show a change in immune competent cell levels and temporary immune restoration of the immune response during treatment with immunostimulators.

B-Lymphocytes↗
PubMed1986

[Scanning electron microscopy study of changes induced by thymomodulin on the morphology of mononuclear elements of peripheral blood from healthy subjects and patients with neoplasms].

The authors studied with scanning electron microscopy the surface of peripheral mononucleated blood cells of healthy subjects and neoplastic patients before and after incubation with thymomodulin, a calf thymus derivative with immunomyelomodulating activity. In the healthy subjects before incubation with thymomodulin a wide number of lymphocytes with villous surface was observed, while monocytes appeared as bigger cells with a central portion of cytoplasm with an adhesion plate. Already after an hour of incubation with the thymic derivative an increase of rough lymphocytes and the appearance of cytoplasmic bridges between lymphocytes and monocytes were observed. Afterwards thymomodulin induced in lymphocytes the forming of cytoplasmic appendages named "uropods" and "pseudopods" which are expression of lymphocytic activation. In cancer patients at base line "monstrous" cells, considered as suffering lymphocytes, were seen and they became morphologically normal after incubation with the thymic derivative.

Adult↗