PubMed Health⌕ Search

Biomedical subjects

D Geissler

Publications and source records attributed to D Geissler.

62 records · Page 4Linked to original sources
PubMed1982

[Proliferation of myeloid precursor cells in a microagar culture system].

A microagar-culture system for the in vitro study of myelopoiesis committed stem cells has been reported. Stimulation was induced by a commercially available conditioned medium obtained from culture of histiocytoma cells. The effect of different concentrations of conditioned medium and a linear relation between the seeded cells and the formed colonies has been established. The granulocytic-monocytic character of cell aggregates has been shown by morphologic studies. Further possibilities for the use of the described method have been briefly discussed.

Agar↗
PubMed1981

A micro-culture system for cloning human T lymphocytes in agar.

A simple and reproducible single-layer micro-agar culture system for cloning of human T lymphocytes has been described. The system consists of an agar layer, in which mononuclear cells from peripheral blood were suspended, and a liquid overlayer containing the mitogenic substance. The advantages of the described method are a low incubation volume (0.5 ml) and the liquid overlayer. The addition of different test substances to the liquid overlayer is simple and easily controllable. Depending on the agar concentration a different number of formed colonies can be found floating in the liquid phase. The morphological, cytochemical and immunological characteristics of the cells from those aggregates could be easily studied. The T-cell characteristics of formed clusters and colonies was confirmed by immunofluorescence and E rosette formation. The effects of agar, serum and cell concentrations, as well as the mitogenic activation caused by three lectins on the development and number of colonies were studied on day 7, 10 and 14 of incubation.

Clone Cells↗
PubMed1981

[Lipid binding in blood clots in acute myocardial infarct].

Lipids of plasma and of clots produced under standardized conditions from platelet poor plasma and from PTT reagent were examined in patients with acute myocardial infarction (AMI) and in normal persons. No differences were found, which excludes a significant influence of fibrinogen, but not of platelets, on clot-lipid binding. The main lipid fractions were therefore also estimated in clots produced from platelet rich native and citrate plasma. These and the individual phospholipid fractions were determined also in the corresponding plasmas and high density lipoproteins (HDL). Plasma of patients with AMI contained more monoglycerides and lecithin but less lysolecithin. This fraction was particularly low in the HDL and corresponded with a decrease of cholesterol esters. Lipid fractions of clots did not differ significantly, whether they were obtained from citrate or native plasma. Clots in AMI contained significantly more triglycerides (TG) suggesting an increased affinity of TG-rich lipoproteins to platelet rich clots. An increased and changed lipid content of clots could inhibit lytic enzymes essential for the removal of clots. HDL cholesterol-esters in AMI were negatively correlated with the main clot lipid fractions.

Adult↗
PubMed1980

[Plasma catecholamines in accidental hypothermia (author's transl)].

The plasma levels of adrenaline and noradrenaline were measured by a radioenzymatic method in 3 patients with accidental hypothermia and followed up until normal body temperature was achieved. In all 3 patients the hypothermia was accompanied by markedly elevated levels of noradrenaline, whereas adrenaline increased considerably only in one of the 3 patients. During normalization of body temperature the elevated catecholamine levels started to decrease. In 2 of the 3 patients nearly normal catecholamine levels were measured, when body temperature had normalized. In spite of the high levels of catecholamines in plasma the heart rate was strikingly low at the lowest temperature. During the increase of the lowered body temperature the heart rate increased in contrast to the decreasing catecholamine levels. The increase of the catecholamine levels in plasma in patients with accidental hypothermia can be explained either by an augmented stimulation of the sympathetic nervous system or by a decreased metabolism. On the basis of the high endogeneous catecholamine levels the use of beta-sympathomimetics appears contraindicated in case a haemodynamic insufficiency develops during the course of accidental hypothermia.

Adult↗
PubMedDate not supplied

Hepatitis A in Western Austria--the epidemiological situation before the introduction of active immunisation.

Several European countries report a decreasing prevalence of antibodies to hepatitis A virus (anti-HAV). This trend is most pronounced in the youngest age groups. In 1979, however, 58% of young Austrians aged 20 to 30 years were shown to possess anti-HAV. Here we describe the current epidemiological situation in western Austria. Prevalence of anti-HAV has decreased to 7% in those 18 to 30 years old. This percentage rises to 20% (31 to 40 years of age) and 57% (41 to 50 years of age) and is highest in those older than 50 years (87%). Of 180 cases of clinical hepatitis A occurring from 1985 to 1992 45% were imported by travel to HAV-endemic areas. Seventy-one percent of the cases in children (59/83) occurred in foreign workers' families and were also predominantly acquired abroad. A change in prevention policy should be considered in this respect, as vaccination is available now.

Adolescent↗
PubMedDate not supplied

Dose escalation of ara-c may improve response rates in a subgroup of chronic myeloid leukemia patients with poor response to interferon-alpha and low-dose ara-C.

The present analysis was performed to evaluate the impact of cytosine arabinoside (ara-C) dose escalation on hematological and cytogenetic responses in patients with chronic myelogenous leukemia (CML) who failed to respond to low-dose ara-C (LD ara-C) at a dose of 10 mg/m2/d over 10 days per month and interferon-alpha (IFNalpha, 3.5 MU/d). Following the same administration schedule, dose escalation of ara-C to 15 and 20 mg/m2/d 1-10 was performed in 36 of 119 patients (30%) due to inadequate hematological response and/or disease progression. As a result, improvement of hematological and cytogenetic responses was achieved in 22 (61%) and nine (25%) patients, respectively. Escalated ara-C dose levels were usually well tolerated, although some patients experienced deterioration of preexisting side effects. Our results support the critical role of ara-C dose towards a better disease control in CML.

Adolescent↗