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Biomedical subjects

D Gendron

Publications and source records attributed to D Gendron.

14 recordsLinked to original sources
PubMed1991

Electrophysiology and electrodiagnosis of the post-polio motor unit.

Post-poliomyelitis syndrome refers to new symptoms that may occur years after recovery from poliomyelitis. The most common of these symptoms are new weakness, fatigue, and pain. This article describes electrodiagnostic studies--conventional electromyography (EMG), single fiber electromyography (SFEMG), and macroelectromyography (macro-EMG)--that have provided information on the post-polio motor unit and on the possible etiology of some post-polio syndrome symptoms. Muscular fatigue, and indirectly, general fatigue, may be due to neuromuscular junction transmission defects in some post-polio individuals, as suggested by reduction of the compound motor action potentials on repetitive stimulation, and increased jitter and blocking on SFEMG. Progressive weakness and atrophy in post-polio syndrome is probably due to a distal degeneration of post-polio motor units with resultant irreversible muscle fiber denervation. Electrodiagnostic evidence of ongoing denervation includes fibrillation and fasciculation potentials on conventional EMG, increased jitter and blocking on SFEMG, and smaller macro-EMG amplitudes in newly weakened post-polio muscles. However, even though electrodiagnostic studies have provided insight into the possible causes of some post-polio syndrome symptoms, no specific electrodiagnostic test for the syndrome is currently available.

Action Potentials↗
PubMed1990

Neurologic sequelae of domoic acid intoxication due to the ingestion of contaminated mussels.

In late 1987 there was an outbreak in Canada of gastrointestinal and neurologic symptoms after the consumption of mussels found to be contaminated with domoic acid, which is structurally related to the excitatory neurotransmitter glutamate. We studied the neurologic manifestations in 14 of the more severely affected patients and assessed the neuropathological findings in 4 others who died within four months of ingesting the mussels. In the acute phase of mussel-induced intoxication, the patients had headache, seizures, hemiparesis, ophthalmoplegia, and abnormalities of arousal ranging from agitation to coma. On neuropsychological testing several months later, 12 of the patients had severe anterograde-memory deficits, with relative preservation of other cognitive functions. Eleven patients had clinical and electromyographic evidence of pure motor or sensorimotor neuronopathy or axonopathy. Positron-emission tomography of four patients showed decreased glucose metabolism in the medial temporal lobes. Neuropathological studies in the four patients who died after mussel-induced intoxication demonstrated neuronal necrosis and loss, predominantly in the hippocampus and amygdala, in a pattern similar to that observed experimentally in animals after the administration of kainic acid, which is also structurally similar to glutamate and domoic acid. We conclude that intoxication with domoic acid causes a novel and distinct clinicopathologic syndrome characterized initially by widespread neurologic dysfunction and then by chronic residual memory deficits and motor neuronopathy or axonopathy.

Animals↗
PubMed1990

Gluteal nerve damage following total hip arthroplasty. A prospective analysis.

Injuries to the peroneal or tobial divisions of the sciatic nerve occur in approximately 0.7-7.6% of patients undergoing total hip arthroplasty. No prior studies have investigated the incidence of injury to the superior or inferior gluteal nerves during hip surgery. This study evaluates the incidence of injury to the superior and inferior gluteal nerves in 55 patients undergoing total hip arthroplasty using a newly devised EMG scoring system. Subclinical gluteal nerve injury was documented in over 77% of patients, whether a posterior or a lateral approach to the hip was used.

Adult↗
PubMed1990

Alternative homologous and nonhomologous products arising from intramolecular recombination.

RmI, a chimeric DNA molecule containing polyomavirus (Py) and mouse sequences, generates unit-length Py DNA via intramolecular recombination between two directly repeated viral sequences of 182 base pairs (S repeats). To analyze the contribution of the S repeats in this process, we produced mutants of RmI carrying deletions in either one or both S repeats and tested them for their ability to recombine in mouse 3T6 cells. Mutant DNAs were found to yield unit-length Py DNA as long as they carried a minimal internal homology of 40 to 50 base pairs. Unlike RmI itself, however, the mutants also gave rise to nonhomologous recombination products. These results suggest that when the generation of homologous products is hampered by a limiting homology, nonhomologous products may arise instead of homologous ones. Therefore, the initial step(s) in the mechanisms yielding the two kinds of products could be identical.

Animals↗
PubMed1990

Preferred crossover sites on polyomavirus DNA.

RmI is a hybrid replicon consisting of polyomavirus (Py) and mouse sequences that yields unit-length polyomavirus DNA via recombination between two directly repeated viral sequences of 182 base pairs (S repeats). To define the contribution of the S repeats in this intramolecular recombination, we derived from RmI a series of replicons containing the original S repeats as well as additional direct viral repeats which were 1 to 2 kilobases in length (L repeats). After mouse 3T6 cells were transfected with these constructs, recombination products that displayed the physical properties of homologous recombinants were detected. The structures of these recombinants indicated that whereas repeat length influences the likelihood of recombination, crossover occurs preferentially near the S repeats, provided that one of them is proximal to the viral origin of replication. This finding suggests that recombination near the S repeats depends on a process initiated near the viral origin of replication.

Animals↗
PubMed1990

Learning caring behavior in an integrated manner.

We can see that by transferring caring behaviors from previous experiences, by imitation of role models, and by multiple experiential activities, learners form and utilize schemata of caring. By finding ways to stimulate learners to think about the feeling of caring and to imagine caring for others, caring becomes a psychophysiological or psychokinesthetic experience. A broad aim or high-level purpose, that is, to care, to have interest in and concern for others, or union and separation toward growth could serve as an inner context that would automatically evoke associated networks of learned, integrated caring actions. The caregiver would not usually be aware of each separate action. These broad aims would organize procedural knowledge, or "know how," in the motor areas of the brain. Let us return to Gardield's predicament. Can we help him walk again? Perhaps someone will call him in a friendly voice, someone whom he knows will give him a little chow and lots of stroking on his head. I suspect he would forget about his feet and just hurry over.

Awareness↗
PubMed1989

Myoclonus epilepsy and ragged-red fibres (MERRF). 2. Electrophysiological studies and comparison with other progressive myoclonus epilepsies.

Electrophysiological studies (EEG, evoked potentials, nerve conduction and EMG) in 13 patients with myoclonus epilepsy with ragged red fibres (MERRF) are presented. The most notable findings are the presence of atypical irregular generalized spike and wave discharges arising from an abnormal EEG background (9 patients), focal epileptiform abnormalities (6 patients) most commonly over the occipital regions and giant cortical SEPs (4 of 6 patients tested). Similar findings of disturbed EEG background activity, generalized spike and wave discharges and giant cortical SEPs appear to be shared by the group of diseases characterized by progressive myoclonus epilepsy.

Adolescent↗
PubMed1988

A substitution in a nonconserved region of polyomavirus large T antigen which causes a thermosensitive mutation.

The temperature-sensitive defect of the tsP155 mutant of polyomavirus (Py) maps in the large T antigen (LT) coding sequence of a viral DNA diverging markedly from that of extensively characterized wild-types (WTs) such as A2 and CSP. We have sequenced about 600 base pairs (bp) "early" DNA encompassing the mutated site in tsP155, as well as the corresponding DNA segment from a revertant virus (RtsP155). As expected, tsP155 was found to be more closely related to CSP than to A2. Out of 3 single bp differences between tsP155 and CSP, 2 were common to tsP155 and RtsP155. The only substitution exclusive to tsP155 was a G----C transversion at bp 2658 which canceled the HaeIII site at bp 2657. Heteroduplexes inclusive of tsP155 DNA and of a 312-bp-long fragment of RtsP155 DNA yielded recombinant viruses growing under restrictive conditions whose DNAs had all regained the HaeIII site at bp 2657. These findings clearly identify the ts mutation with the tranversion at bp 2658, which is expected to change Ala 701 for a Pro in LT. We discuss this substitution in relation to the phenotype of tsP155.

Amino Acid Sequence↗
PubMed1986

An amplified genome that may have resulted from recombination within bidirectionally replicating DNA.

Temperature shift-down of permissive mouse cells transformed by a temperature-sensitive (ts) polyomavirus (Py) genome has been shown to induce the accumulation of free copies of the viral DNA. We report here on an unusual product from such induction. The structure of this product is that expected from the occurrence of recombination between growing points in a bidirectionally replicating Py-mouse DNA molecule. This observation may be relevant to the mechanism of gene amplification in mammalian cells.

Animals↗
PubMed1986

An excision event that may depend on patchy homology for site specificity.

In mouse cells transformed by a mutant polyomavirus genome, recombination between integrated viral DNA and flanking cellular DNA resulted in the excision of two readily amplifiable chimeras, designated RmI and RmII. The crossing-over that generated RmII was unique in that it involved a simple cellular sequence in which the triplet 5'-CTG-3' was repeated many times. We show that the sequence across the junction resulting from excision was identical in several molecules of RmII, as if the cross-over generating this junction always involved exactly the same two sites on the viral and cellular DNA. We also show that the cellular site mapped where the replacement of a G by an A in one of many successive 5'-CTG-3' triplets generated a homology of five nucleotides (5'-CTACT-3') with the viral site. Oligonucleotides on both sides of these sites are probably involved in matching the two DNAs prior to recombination.

Animals↗
PubMed1985

Mouse cells surviving polyoma virus infection generally retain the whole viral genome.

Permissive mouse 3T6 cells were exposed to polyoma virus--either wild-type or early mutant--at high multiplicities of infection. From colonies arising from surviving cells, so-called lines and clones were derived under conditions precluding superinfection. These lines and clones were examined for the presence of viral genetic information, using a variety of techniques. Two salient findings were made: most lines or clones analyzed had retained viral genetic material; generally, this material was nondefective, as evidenced by the production of virus and/or viral DNA molecules of genomic size. These findings indicate that mouse cells can survive for many generations while carrying a complete, infectious, and potentially cytocidal polyoma virus genome.

Animals↗
PubMed1978

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