Biomedical subjects
Publications and source records attributed to D Gieracka-Pazucha.
Current opinions connected with the role and significance of ubiquitous proteins--ubiquitins (Ub) were discussed. Genetic determination of ubiquitins and their expression in nature were considered. Thymic ubiquitin complex, as an extract from calf thymus gland (TFX) was also presented. Very important biological activities in some experimental models and usefulness of TFX in medical practice were presented.
Subpopulations CD3+, CD4+ and CD8+ of T lymphocytes in 67 healthy individuals and in 36 sensorineural hearing loss (SNHL) patients with unknown etiology were determined. The patients were studied in two groups, i.e. 29 patients with bilateral and progressive hearing loss and 7 patients with sudden deafness. The ratio of CD4+ and CD8+ was also calculated. The results were presented as the mean value for percentage and absolute numbers of the cells together with statistical analysis. The acquired results of two group patients with SNHL were completed before and at the beginning of ubiquitine therapy. The highly significant differences (p < 0.001) for CD8+ subset in comparison with the results of control group was found. The restitution effect of ubiquitine treatment for CD3+ and CD4+ T lymphocytes subpopulation in SNHL patients was observed. Additionally in patients with sudden hearing loss the CD8+ subset was also normalized. The ratio of CD4+:CD8+ in patients with bilateral and progressive hearing loss was lower (1.27 +/- 0.21) that in control group (1.66 +/- 0.25) and significantly different (t = 7.31; p < 0.001). It was shown that the distribution of examined CD system markers which depended on CD4+:CD8+ ratio was normal only in 27.6% of patients with SNHL. It was a group of 9 patients in whom hearing loss appeared after bacterial or virus infections. No changes in the CD4+:CD8+ subpopulation ratio at the beginning of ubiquitine therapy was observed. The distribution of normal results for T cell subpopulations in patients before and during treatment was presented in table III. Our results indicate that the observations may have an important diagnostic value. The authors suggest that the CD system antigens determination in monitoring the clinical status of SNHL patients and also in patients treated with drugs influencing the immune system is needed.
Receptors activity of peripheral T cells for sheep erythrocytes was analyzed in 67 healthy individuals and in the 29 patients with progressive, bilateral hearing loss and in 7 patients with sudden deafness on unknown etiology. The ability of T lymphocytes to form non-immune complete and incomplete rosettes in TEt and TEa tests was evaluated before and at the beginning of ubiquitine therapy. The mean values of rosette forming cells (RFC) in percentage and absolute numbers and the statistical analysis were presented. It was found that the receptor activity of T lymphocytes in TEt test was not significant different from the results of healthy control group. On the other hand highly significant differences were observed (p < 0.001) in subset of "active" T-cells determined in TEa rosette test. The relative coefficient of receptor activity of lymphocytes (CRAL) calculated for complete TEa rosettes was high different (p < 0.0008). Normalization of the results of TE rosette tests after first period of ubiquitin biotherapy was observed.