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Biomedical subjects

D Granda

Publications and source records attributed to D Granda.

8 recordsLinked to original sources
PubMed1996

[Applications of the polymerase chain reaction (PCR) to the diagnosis of central nervous system infections].

The utility of polymerase chain reaction (PCR) is described for the diagnosis in three patients suffering from central nervous system infections, tuberculous meningitis, herpetic encephalitis and cerebral toxoplasmosis. PCR was performed in the cerebrospinal fluid after processing the specimen by two methods, proteinase K digestion and phenol extraction of DNA. Amplification was realized using primers previously described that amplify specific DNA fragments of each microorganisms (insertion sequence IS6110 of Mycobacterium tuberculosis, B1 gene of Toxoplasma gondii, and DNA polymerase gene of Herpes simplex virus). In all three cases, PCR was positive after amplification of the specimen extracted with proteinase K, as well as when a complete DNA extraction with phenol was realized. In all cases a band of amplified products was observed in agarose gels. In conclusion, in all three patients described, PCR would had allowed the diagnosis in seven hours, and PCR should be consider a rapid sensitive and relatively simple method.

AIDS-Related Opportunistic Infections↗
PubMed1995

The utility of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis.

A fragment of DNA of 123 bp belonging to insertion sequence IS6110, specific of Mycobacterium tuberculosis complex, was amplified by polymerase chain reaction (PCR) of respiratory samples, for the diagnosis of pulmonary tuberculosis. A total of 314 samples (286 sputum and 28 bronchoalveolar lavages) from 242 patients were evaluated by PCR, and the results were compared with the those obtained by acid-fast-stained smears, culture, and clinical diagnosis. Mycobacterium tuberculosis was detected by PCR in 102 of 105 patients with clinical diagnosis of pulmonary tuberculosis. All smear and culture-positive samples were PCR positive. The sensitivity of PCR, culture, and staining was 97%, 88%, and 65%, respectively, and the specificity was 100% in all cases. In ten patients with old residual lesions, but no active disease, M tuberculosis genome was detected by PCR. In our experience, PCR proved to be a useful method for the rapid diagnosis of pulmonary tuberculosis.

Adolescent↗
PubMed1993

[Usefulness of IgG and IgM detection against antigen 60 in the diagnosis of thoracic tuberculosis].

We conducted a prospective study in order to assess the use of the "ELISA" method (Anda-Tb) for the detection of antibodies IgG and IgM against antigen 60 in mycobacterias for the initial diagnosis of thoracic tuberculosis. 215 serum samples from 44 patients with tuberculosis and 171 control cases were studied. The threshold value for IgG in our environment is 200 U, resulting in a specificity of 98% and a sensitivity of 34%. The IgM test has a low sensitivity, although when combined with the IgG, the sensitivity of the test increases while its specificity is reduced. This method is not useful in patients with HIV infection and immunodepression (AIDS). We have not observed any relationship between the serology and the response to PPD intradermorreaction. We conclude that this method could be used in our environment as a supplementary test, but in any case as a substitute of the traditional microbiological diagnosis.

Adolescent↗
PubMed1991

[Mumps encephalitis in adulthood].

Mumps virus is one of the most common causes of viral meningitis. Although brain involvement has been observed in a low proportion of children with mumps meningitis, a pure form of mumps encephalitis is extremely uncommon in the adult. A 23 year-old man presented with a rapidly evolving syndrome of cephalalgia , vomiting, mutism, disorders of gait, somnolence and dystonic movements. The electroencephalogram showed a diffusely slowed background activity. The CSF contained no cells, but the total protein concentration was elevated. The patient recovered without sequelae, but severe intellectual and motor disturbances persisted during more than a month. Serological studies showed an increase of blood IgM mumps-specific antibodies. We conclude that mumps encephalitis, although infrequent, should be considered among viral diseases that mimic herpes simplex encephalitis in the adulthood.

Adult↗
PubMedDate not supplied

[Prevalence of HIV antibody carriers in patients assisted in the recovery room].

A study has been performed on 200 patients at the Recovery Department of our hospital during 1987. This study was carried out in order to determine patients with human immunodeficiency virus (HIV) antibody. This group was randomized and consisted of 154 men and 46 women, with an average age of 35.6 +/- 3.9, a total weight of 68 +/- 6 kg and a height of 1.69 +/- 0.2 m. The day entered at the Recovery Department we assessed the following parameters: a) addiction to drugs by parenteral way; b) bleeding; c) invasive procedures; d) etiology, and e) blood samples were drawn for plasma antibody to HIV. We detected five patients (2.5%) with antibody anti-HIV and all of these patients were male and they were aged in 20-39 years old. We noticed a close relation between addiction to drugs and HIV (p less than 0.001), nevertheless no relation has been found between invasive procedures, bleeding, etiology and antibody to HIV. We conclude that the number of patients that we detected with antibody from the HIV is similar to those found by other studies that has been carried out in emergency situation, but greater than those found in the screening of the general population.

Acquired Immunodeficiency Syndrome↗