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Biomedical subjects

D Grimm

Publications and source records attributed to D Grimm.

At least 55 records · Page 3Linked to original sources
PubMed1995

Visualization of Müller (retinal glial) cells by bulk filling with procion yellow.

A method is presented that allows for an easy and reliable demonstration of retinal glial (Müller) cell morphology. When a 3% solution of the fluorescent dye Procion Yellow (reactive yellow, Sigma) is placed on isolated living retinae for 2 hrs, many Müller cells take up the dye. In paraffin sections, the cells can be observed by confocal microscopy in great detail. As the cells are filled throughout their length, the method has advantages over most immunocytochemical methods which label only parts of the cells. The method was applied to retinae of frogs, rats, guinea pigs, and rabbits. The vitread trunks of the cells differed in diameter. Those of frogs and rats were thin (less than 1 to 2 microns diameter) whereas those of guinea pigs and rabbits were thicker (2 to 5 microns). In all species studied the following rule was found. In thick central regions of the retina, Müller cells were long with slender trunks whereas in the thin retinal periphery, the Müller cells had thick short trunks. There was an inverse relationship between length and diameter of Müller cell trunks. Mammalian Müller cells were densely packed and had rather cylindrical endfeet. In the frog retina, Müller cells were more sparsely distributed, and the endfeet formed wide, flat funnels. It is concluded that the higher metabolic rate of mammalian retinae requires more densely packed Müller cells than occur in the amphibian retina.

Animals↗
PubMed1994

Proliferation of tumor spheroids after shock-wave treatment.

Multicellular tumor spheroids (MCTS) grown from the bladder cancer cell line RT112 and from the prostate cancer cell line PCA were exposed to 200 or 800 electromagnetically generated focused ultrasound shock waves. RT112 cells showed a distinct but transient decrease in proliferation whereas the effect of PCA cells was less pronounced. Flow-cytometric measurements of DNA content and Ki67 expression revealed no significant changes in the cell cycle distribution. The capacity of RT112 cells exposed to 800 shock waves to re-grow as MCTS was markedly decreased, indicating an alteration of intercellular adhesion.

Cell Division↗
PubMed1992

Establishment and characterization of a human papillary thyroid carcinoma cell line with oxyphilic differentiation (ONCO-DG 1).

In the present study the establishment and characterization of a new oxyphilic papillary thyroid carcinoma cell line--ONCO-DG1- is given. With immunohistological, histochemical and flow cytometric methods, ONCO-DG 1 cells revealed features of epithelial differentiation. Furthermore the cells formed von Kossa-positive deposits resembling psammoma bodies in monolayer and spheroid culture until late passages. The tumor cell line is now in the 40th subculture. Because of the ability to form multicellular tumor spheroids (MCTS), this cell line is a good model for examining the interaction between thyroid tumor cells and confluent human endothelial cells on extracellular matrix in vitro. It is also suitable for xenotransplantation studies, because it is tumorigenic in NMRI nude mice in vivo.

Animals↗
PubMed1990

[Computer scintigraphic investigations of function of large salivary glands].

With the help of a gamma scintillation camera and a connected data manipulation system using the radioisotope 99mTc pertechnetate the large salivary glands of the head of 44 patients without pathological history of the salivary glands were examined by the results were interpreted. Because of the extended interval it was not able to ascertain normal values with clinical usefulness of the radionuclide accumulation in the large salivary glands. The time-activity-profiles which were won above "regions of interest" were very variably. The patients were divided classify and by using statistical test we found nonsecure influence of age or diabetes mellitus on the function of the salivary glands. Three types of normal time-activity-profiles were differed. In consequence of the discrepancy between the technical expance and the diagnostic value these method will to reserved to a limited range of indication outside the routine diagnostic.

Adult↗
PubMed1986

Norepinephrine-induced acute renal failure: beneficial effects of atrial natriuretic factor.

The effect of the atrial natriuretic factor (ANF) on early norepinephrine-induced acute renal failure (ARF) was investigated. In anaesthetized female Sprague-Dawley rats, weighing 247 +/- 36 g, ARF of the left kidneys was induced by 40-min intrarenal arterial infusion of norepinephrine (NE; 0.75 micrograms/kg body weight). In each case the right kidney served as a control organ. Inulin clearance was used as an estimate of glomerular filtration rate (GFR). Urine volume (V), GFR and fractional excretion rates of sodium, potassium and chloride were studies in both kidneys. Immediately after NE-induced ARF one group received ANF (alpha-hANaP) and the other groups 5% glucose or isotonic saline; all infusions were administered intrarenally. In the experimental kidney NE-infusion induced anuria. ANF infusion was able to induce complete reversal of the NE effect, and GFR and diuresis improved markedly in the experimental kidney. In addition, a tremendous rise in fractional excretion rates of sodium and potassium after administration of ANF was observed. In the control kidney an additional increase of GFR and diuresis was seen. Following 5% glucose or isotonic sodium chloride infusion, no profound effects were obtained in both kidneys. The results of the present study demonstrate that ANF provides beneficial effects on the functional damage of NE-induced ARF. We conclude that the NE-antagonistic effect of ANF and the consecutive amelioration of GFR may play an important role in the recovery of impaired renal function.

Acute Kidney Injury↗
PubMed1986

Human atrial natriuretic factor prevents against norepinephrine-induced acute renal failure in the rat.

The aim of the present study was to investigate the potential protective effect of atrial natriuretic factor (ANF) on the norepinephrine (NE)-induced acute renal failure (ARF). After determination of basal renal function, ANF at different doses induced a dose dependent increase of glomerular filtration rate (GFR), diuresis (V), sodium (UNaV) and potassium (UKV) excretion. Unilateral intrarenal norepinephrine infusion induced acute renal failure in an isotonic saline pretreated rat group. The experimental kidney in this group was anuric, and no measurable GFR and electrolyte excretion was observed. ANF pretreatment prevented the norepinephrine-induced ARF. In the experimental kidney, GFR, V, UNa V and UK V were subnormal or even elevated during NE-infusion as compared to the basal clearance period. During the 60 min control period after the norepinephrine infusion in the isotonic saline pretreated rat group, anuria persisted. In the ANF pretreated groups, normal or significantly increased levels of GFR, V, UNa V and UK V were obtained.

Acute Kidney Injury↗
PubMed1985

[Treatment of ovarian cancer with combined operative, radiologic and cytostatic therapy].

It is reported about significant superior results of two years of the combined ovarian cancer treatment in contrast to operation and chemotherapy. Our therapy is the combination of operation, great field radiation technique, chemotherapy and second-look-operation. The remission of two years: Stage I: 64%, stage II: 50%, stage III: 45% and stage IV: -Only in stage IV no improvement can be achieved by addition of the radiation therapy.

Adult↗
PubMed1985

[Clinical experience with short-term afterloading therapy in comparison with conventional brachytherapy in the treatment of gynecologic tumors].

The short-term afterloading therapy (AL-ST) with high dose rates (DR) and remote control prevents the risk of a radiation exposure of the staff, facilitates the optimization of the dose distribution in space, makes the treatment easier for patients and hospital, and allows a considerable increase of the treatment capacity without additional need of staff or capital. AL-ST works with another dose distribution in time than the conventional brachytherapy, so a higher fractionation of high-dose-rate afterloading is substituted for the classical protraction of low-dose-rate brachytherapy. 2072 patients with gynecologic tumors were treated by AL-ST between 1974 and 1983. 1762 out of them (964 carcinomas of the cervix, 677 carcinomas of the body, and 121 vaginal tumors, metastases and urethral carcinomas) could be checked up for at least twelve months up to more than five years, which allowed an evaluation with regard to recurrence-free survival rate, local absence of tumors, and side effects. The five-year survival rates obtained by primary and post-operative AL-ST are compared to historical control groups of our own hospital and to the international results. The results, related to the stages, are at least equivalent; several groups show a statistically significant improvement compared to conventional brachytherapy. The incidence of early and late reactions in bladder and rectum showed a statistically significant decrease after AL-ST and was dependent on the dose in a statistically highly significant manner (p = 0.001). In addition to the well-known advantages of AL-ST, the following may be mentioned: 1. The intracavitary application was made without general anaesthesia, only with sedation by drugs, which prevented the primary treatment mortality. 2. An ambulatory treatment was possible in about 40% of the cases due to the time-sparing and patient-sparing method--the advantages are evident. 3. The therapeutic efficacy is increased and the risk of side effects in bladder and rectum is decreased by the better radiobiologic (same DR) and dosimetric adaption of AL-ST and percutaneous high-voltage therapy.

Ambulatory Care↗