Biomedical subjects
Publications and source records attributed to D H Carver.
Preparations of crude leukocytes from rabbit peritoneal exudates contain interferon-like activity which may be separated from pyrogen activity both by Sephadex chromatography and by purification of the pyrogen. This indicates that, despite some similarities, leukocyte pyrogen is not an interferon.
Explore the source record for details and available documents.
Unlike other strains tested, the RA 27/3 vaccine strain of rubella virus, attenuated in human fibroblasts, failed to inhibit superinfection with Newcastle disease virus (i.e. induce intrinsic interference). Since proteins of the input virion are known to lead to intrinsic interference, these may differ in the RA 27/3 strain from those in other rubella strains.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Exposure of human Wi-38 cells to human serums containing Australia antigen, and presumably serum hepatitis virus, renders the cells refractory to infection by Newcastle disease virus as detected by the hemadsorption-negative plaque test for intrinsic interference. Induction of the Newcastle disease virus refractory state could be passed in cell culture with up to a 1 : 100,000 dilution of material obtained from cells "infected" with serums containing Australia antigen after filtration (0.45-microm pores) and heating to 60 degrees C for 1 hour. Human antiserums to the Australia antigen prevented induction of the Newcastle disease virus refractory state.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Cytomegalovirus (CMV) was demonstrated to induce intrinsic interference to Newcastle disease virus (NDV) in human fibroblast cells under noncytopathic conditions. This interference is unique in that (i) cytomegalovirus is the first DNA virus demonstrated to have this property and (ii) the state of interference was transient and progressively lost as the condition of the cells changed with the development of cytopathic effect. These observations are consistent with the view that the newly formed protein responsible for interference with NDV has a limited half-life and is no longer made when cytopathic conditions are produced by CMV.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
A hybrid mouse-hamster cell line was developed from a mouse cell line which produces a high titer of interferon and is sensitive to its action, and a hamster cell line which produces little interferon and is relatively insensitive to its action. Parental cell lines demonstrated complete species specificity with respect to interferon production and action. The hybrid cells produced interferon (or interferons) effective when tested on the mouse cell line and primary hamster cells; the hybrids were sensitive to the action of both mouse and hamster interferons. Hybrid cells produced ten times more hamster interferon than the parent hamster cell line and were eight times more sensitive to hamster interferon than the parent hamster cell line.