Biomedical subjects
Publications and source records attributed to D H Read.
Direct amplification and sequencing of the 16S rRNA gene and a variable region of the flagellin gene from fetal liver-associated spirochetes belonging to the Borrelia parkeri-B. turicatae tick-borne relapsing fever spirochete group with a late-term abortion in a mare are described.
Hepatitis-splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the genetic identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30-35 nm in diameter. The sequence of the 3' half of the viral genome ( approximately 4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3' non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58-61% aa and 57-60% nt sequence identities. The RdRp gene of avian HEV shares 47-50% aa and 52-53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48-49% aa and 48-51% nt sequence identities with the corresponding regions of other known HEV strains. Phylogenetic analysis indicates that avian HEV is genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.
A cross-sectional study was conducted in southern Chile between January and March, 1996, to identify risk factors for papillomatous digital dermatitis (PDD) in lactating dairy and dual-purpose cows. A total of 3,265 cows from 22 farms were examined in the milking parlor for PDD lesions. Additional information was collected from dairies' computerized records and by direct interview of managers. Data were analyzed using logistic and logistic-binomial regression (with dairy as a random-effect term). German Red-Pied (dual-purpose) cows were significantly (P < 0.05) less likely (odds ratio (OR) = 0.3) to have PDD lesions than German Black-Pied and Holstein crossbreds. First-parity cows had the highest odds of PDD, and odds diminished, in a dose-effect manner, as parity increased. Odds of PDD increased with increasing days in lactation. Cows that calved during winter were more likely to have PDD (OR = 1.4) than those calving at any other season. Cows on farms that bought heifers in the past 10 years had a 3-fold increase in the odds of PDD compared to those on farms that never bought heifers. Loose-housed cows had a higher risk of PDD (OR = 7), followed by cows in free stalls or in open corrals (OR = 2.8 and 1.3, respectively), compared to cows on pasture all year. Cows on dairies that used a footbath during 1996 were less likely (OR = 0.3) to have PDD than those in dairies not using one. Parlor type was associated with PDD, but this was likely an effect of parlor design on ease of inspection of cows' feet. A policy of trimming all cows' vs. only lame cows' feet and a policy about buying adult cows did not have significant effects on PDD risk.
Outbreaks of papillomatous digital dermatitis (PDD) have been reported from many countries, but there is little information on prevalence and geographic distribution of the disease. Our objectives were to describe prevalence of PDD on dairies belonging to the two main dairy-producer associations in Chile, and to evaluate a milking-parlor screening method for detection of PDD in dairy cows. First, a self-administered questionnaire was sent to all 214 association dairy managers. Seventy percent of the 119 respondents had observed PDD on their dairies. About 83% of the 63 responding managers had first seen PDD during the previous 10 years. Subsequently, a random sample of 43 dairies stratified by association was selected from the total 214 for PDD screening. During milking, we inspected cows' feet for PDD lesions; 91% of dairies had cows affected by PDD; median PDD prevalence for milking cows in dairies was 6.1%. Finally, in one dairy, PDD screening at the parlor was compared to PDD diagnosis by inspecting restrained cows in a chute. The screening method had a sensitivity of 0.72, and a specificity of 0.99. This study demonstrated that PDD was widespread on study dairies in Chile. The screening test presented can be used as a cost efficient method to estimate prevalence of PDD either on individual dairies or in large-scale surveys.
OBJECTIVE: To determine whether a two-month or longer period without official high-speed workouts (lay-up) is associated with humeral or pelvic fracture in Thoroughbred racehorses. DESIGN: Reprospective study. ANIMALS: Thoroughbred racehorses in California that were euthanatized because of a complete humeral or pelvic fracture. PROCEDURE: Age, sex, activity, number of lay-ups, number of days from a race or official timed workout to fracture, number of days from end of last lay-up to fracture, mean duration of lay-ups, and total number of days in race training were compared between horses with humeral fractures and horses with pelvic fractures. A case-crossover study was used to estimate relative risk for fracture of the humerus or pelvis occurring within hazard periods of 10 and 21 days following lay-up, compared with periods following more regular participation in official racing or timed workout events. RESULTS: Horses with pelvic fractures were more often female, older, and had 0 or > or = 2 lay-ups. Horses with humeral fractures were typically 3-year-old males that had 1 lay-up. Horses with pelvic fractures had more total days in race training, fewer days from last exercise event to fracture, and a greater number of days from end of last lay-up to fracture than horses with humeral fractures. Return from lay-up was strongly associated with risk for humeral fracture during hazard periods of 10 and 21 days (relative risk = 71 and 45, respectively). CLINICAL IMPLICATIONS: Risk of humeral fracture may be reduced if horses are cautiously reintroduced into race training after lay-up.
OBJECTIVE: To investigate relationships of several racehorse characteristics and race conditions with risk of a catastrophic musculoskeletal injury (CMI) resulting in euthanasia in Thoroughbreds during racing in California in 1992. DESIGN: Retrospective longitudinal study. ANIMALS: Thoroughbreds that incurred CMI during racing and all California race entrants in 1992. PROCEDURE: Necropsy records were reviewed, and race start information was obtained. Incidence risk of CMI/1,000 race entrants was estimated. Relationships between CMI during racing and race-meet, entrant age and sex, race type and length, and racing surface type and condition were evaluated by use of logistic regression. RESULTS: Incidence risk of CMI was 1.7/1,000 entrants. A higher risk of CMI was found at 2 fair race-meets, with incidence risks of 4.9 and 5.5/1,000 entrants. Risk of injury in male horses was 1.7 times greater than that in female horses, and influence of age on risk depended on race type. Risk of injury for horses 2 to 5 years old was two times greater for claiming horses than for maiden horses. Race length or racing surface type (dirt vs turf) or condition (fast, muddy, yielding) were not significantly associated with risk of CMI. CLINICAL IMPLICATIONS: Incidence of CMI was similar among 12 of 14 major and fair race-meets and among various race lengths and racing surface types and conditions, whereas incidence of CMI was influenced by entrant age and sex as well as race type. Investigators should consider controlling for age and sex, race-meet, and race type whenever possible in studies of risk of CMI.
Clinical, gross pathologic, and therapeutic studies were performed on a contagious, painful, wart-like digital disease of unknown etiology in California dairy cattle. The disease was called papillomatous digital dermatitis (PDD). Survey indicated that the disease spread geographically throughout southern California over the past few years. In 1991, 31% of herds had papillomatous digital dermatitis, whereas in 1994, 89% were affected. Increased incidence occurred during late spring and summer, 1-3 months after the rainy season. Within-herd morbidity ranged from 0.5% to 12% per month. Study of 93 cows in 10 drylot dairies revealed that 91% had characteristic circumscribed, erosive to papillomatous, intensely painful lesions often surrounded by a ridge of hyperkeratotic skin bearing hypertrophied hairs. Lesions were 2-6 cm across (88%), circular to oval (78%), and raised (59%) and had surfaces that were uniformly erosive and granular (31%), uniformly papillary (28%), or composites of both appearances (41%). Lesions were most frequently seen in lactating heifers (31%) and 3-year-old cows (43%). Clinical signs were characterized by lameness, with walking on toes and clubbing of hooves. Lesions exclusively involved the hind limbs in 82% of cows and the plantar/palmar regions in 84% of cows. Lesions had high (89%) prediliction for plantar/palmar skin bordering the interdigital space. Lesions exclusively involved either the medial or lateral digit in 10% and 28% of cows, respectively. In 50% of cows, both medial and lateral digits of individual limbs were involved; in most cows (31%), lesions apposed each other across the plantar interdigital space, whereas in others (19%), lesions confluently involved the entire plantar/palmar commissural skin folds. In another 12% of cows, lesions were axial. High proportions of lesions showed complete therapeutic responses to antibiotics: parenteral penicillin (9/9) and ceftiofur (41/44), and topical oxytetracycline (4/4). Recurrence or new lesion development occurred in 48% of cows reexamined 7-12 weeks after complete therapeutic response was observed. Overall, the findings indicated that PDD is a distinct disease entity of economic importance in which bacteria may play an important pathogenic role.
OBJECTIVE: To evaluate hoof size, shape, and balance as risk factors for catastrophic musculoskeletal injuries (CMI), including suspensory apparatus failure (SAF) and cannon bone condylar fracture (CDY) in Thoroughbred racehorses. ANIMALS: 95 Thoroughbred racehorses that died between 1994 and 1996. PROCEDURE: 38 quantitative measures of hoof size, shape, and balance were obtained from orthogonal digital images of the hoof and were compared between case horses with forelimb CMI (70), SAF (43), and CDY (10) injuries and control horses whose death was unrelated to the musculoskeletal system (non-CMI, 25). Comparison of group means between cases and controls was done using ANOVA, and multivariable logistic regression was used to estimate odds ratios. RESULTS: Odds of CMI were 0.62 times lower for a 5mm increase in ground surface width difference and 0.49 times lower for a 100-mm2 increase in sole area difference. Odds of SAF were 6.75 times greater with a 10 degrees increase in toe-heel angle difference and 0.58 times lower with a 100-mm2 increase in sole area difference. Odds of CDY were 0.26 times lower with a 3 degrees increase in toe angle, 0.15 times lower with a 5-mm increase in lateral ground surface width, and 0.35 times lower with a 100-mm2 increase in sole area difference. CLINICAL RELEVANCE: Decreasing the difference between toe and heel angles should decrease risk of SAF for Thoroughbred racehorses and should be considered in addition to increasing toe angle alone to help prevent catastrophic injury. Trimming the hoof to perfect mediolateral symmetry may not be a sound approach to avoiding injury.
OBJECTIVE: To characterize history, clinical signs, and pathologic findings in horses with histologically confirmed acute hemorrhagic pulmonary infarction and necrotizing pneumonia. DESIGN: Retrospective study. ANIMALS: 21 horses. RESULTS: 19 of the 21 horses were Thoroughbred racehorses in training. Eighteen horses had had strenuous exercise immediately prior to onset of illness. Fifteen horses had a serosanguineous nasal discharge during hospitalization. Seventeen horses had radiographic evidence of pulmonary consolidation and pleural effusion. Nine of 14 horses had ultrasonographic evidence of large pulmonary parenchymal defects consistent with consolidation. Pleurocentesis yielded a suppurative, serosanguineous effusion in the 14 horses in which it was performed. Bacteria were isolated from all transtracheal aspirates (14) and from 6 of 12 pleural fluid samples. Actinobacillus suis-like organisms and Streptococcus equi subsp zooepidemicus were most commonly isolated. Nineteen horses were hospitalized and treated. Mean duration of treatment was 5 days, and most horses were euthanatized because of secondary complications, continued costs of medical treatment, or poor prognosis for future performance. Pathologic lesions included well-demarcated regions of hemorrhagic pulmonary infarction with necrosis and a serosanguineous pleural effusion. Thrombosis of pulmonary vessels was found in 11 horses. CLINICAL IMPLICATIONS: An acute or peracute onset of severe respiratory distress, with serosanguineous nasal discharge, ultrasonographic and radiographic evidence of severe pulmonary consolidation, and serosanguineous suppurative pleural effusion, is strongly suggestive of pulmonary infarction in horses. Horses with pulmonary infarction responded poorly to conventional treatment for pleuropneumonia and had a poor prognosis for recovery.
This retrospective study of papillomatous digital dermatitis (PDD) was performed using data for cows that calved between July 1993 and June 1994 on a Mexicali, Mexico, dairy farm in order to calculate incidence rates, and to compare milk yield and reproduction data between affected and nonaffected dairy cows. A total of 190 out of 577 (33%) cows were affected during lactation and six (1%) during the dry period. Fifty-two of 77 (68%) cows that had lesions in the previous lactation and were also present on the farm in the current lactation had lesions in the current lactation. The highest risk for PDD occurred during the first month of lactation (9%). The yearly estimated cumulative incidence risk was 35% and the incidence density rate was 44.6 cases per 1000 cow-months. More animals were affected in summer and fall than in winter and spring. Purchased animals were 3.4 times more likely to be affected than animals born on the farm. Survival analyses indicated healthy cows conceived 93 days after calving (median), but affected cows conceived 113 days after calving (median) (P < 0.01). PDD-affected animals produced less milk than healthy cows, but the difference was not statistically significant in the multiple regression.
OBJECTIVE: To determine whether a humoral response against spirochetes isolated from papillomatous digital dermatitis (PDD) lesions is elicited in dairy cattle affected with PDD. SAMPLE POPULATION: 41 cattle with PDD from 8 dairies (study population) and 30 cattle from 2 dairies free of PDD (control population). Additionally evaluated were 32 cattle from a dairy with a past history of PDD but no current disease, and 52 cattle from a dairy with high prevalence of PDD, 25 with and 27 without detectable lesions. PROCEDURE: ELISA were used to evaluate the humoral response of all cattle to representative isolates from 2 groups of spirochetes of unknown species isolated from PDD lesions. Specificity of the response was evaluated, using immune sera prepared against each of the spirochetes, and by adsorption studies of immune and field sera. The potential for confounding by an antibody response to other spirochetes associated with diseases of cattle was assessed. RESULTS: The antibody response (specific) to both PDD spirochete groups of cows with PDD was significantly increased, compared with that of cows from PDD-free dairies. There was no association between antibody response to PDD-associated spirochetes and antibody response to other spirochetal diseases of cattle. None of the cattle from the dairy with previous history of PDD but without current disease were classified as test positive by either PDD ELISA. There was a significant (P < 0.01) difference in classification results for both PDD ELISA for cattle with PDD from the dairy with a high herd prevalence of PDD, compared with cattle without detectable disease from the same dairy. CONCLUSIONS AND CLINICAL RELEVANCE: The humoral response in cattle with PDD lesions was significantly different from that in cattle without detectable lesions, thus providing additional information regarding the potential role of spirochetes isolated from PDD lesions in the etiopathogenesis of PDD.
OBJECTIVE: To estimate prevalence of papillomatous digital dermatitis (PDD) in California dairies during 1993, to describe temporal and spatial patterns of PDD and response of cows to treatment, and to evaluate herd-related risk factors for PDD. DESIGN: Epidemiologic survey. SAMPLE POPULATION: All southern California dairies and a 50% random sample of the rest of California dairies. PROCEDURE: A questionnaire was mailed to managers of 1,429 selected dairies. RESULTS: Prevalence of PDD during 1993 was significantly higher for dairies in the south (75.3%) and central (68.8%) regions, compared with north coast (33.3%) and north (23.1%) regions of the state. Herd mean and median proportions of affected cows in PDD-affected herds were 11.6 (SEM = 0.9) and 5%, respectively. Most (74%) dairy managers surveyed observed PDD for the first time during 1992 or 1993. Highest PDD activity was reported as taking place in summer in the south and in fall and winter in the north coast and north regions; an obvious pattern was not determined for the central region. CLINICAL IMPLICATIONS: PDD was widespread in California dairies during 1993 and affected a high proportion of cows, especially in the south and central regions of the state.
OBJECTIVE: To characterize and contrast data from Thoroughbreds that incurred a fatal musculoskeletal injury (FMI; injury resulting in death or euthanasia) during racing or training and data from all California race entrants during a 9-month period in 1991. DESIGN: Case-control study. ANIMALS: Thoroughbreds that incurred a FMI during racing or training at a California race-meet and all California race entrants from January through June and October through December 1991. PROCEDURE: Age and sex were compared with chi 2 and Fisher's exact tests among horses fatally injured while racing and training. A log-linear model was fit to assess the relationship between race-meet and age and sex of California race entrants. Incidence risk of racing FMI was estimated per 1,000 race entrants, and the relationship between the occurrence of FMI during racing with race-meet, age, and sex was evaluated by logistic regression. RESULTS: Injury type and sex-specific age distributions differed among the horses fatally injured during racing and training. Age and sex distributions of the race entrants were not independent and varied among race-meets. Overall incidence risk of racing FMI was estimated at 1.7/1,000 race entrants. Risk of racing FMI in male horses was about twofold that in female horses, and in 4-year-olds was twofold that in 3-year-olds. CLINICAL IMPLICATIONS: Age and sex-related differences in risk of incurring a FMI during racing should be considered when comparing fatal injury rates among race-meets.
OBJECTIVES: To develop a standard technique for evaluation of racehorse shoes, to assess homotypic variation (interlimb variation) in shoe characteristics, and to determine whether shoe characteristics varied with age and sex. DESIGN: Cross-sectional study. ANIMALS: Thoroughbred racehorses (n = 201) that died or were euthanatized at California racetracks between August 1992 and July 1994. PROCEDURE: Shoe characteristics were measured on horses examined after death. Percentage of agreement was used to compare shoe characteristics between limbs (homotypic variation). Using chi 2 analysis, shoe characteristics were compared between horses grouped by age and sex. RESULTS: Toe grabs were present on 90.5% of horses, and rim shoes were present on 15.9% of horses. Heel traction devices were less frequent on front (2.5%) than rear (6%) hooves. Pads were present on 24.9% of horses, with bonded rim pads most common. Special types of shoes were present cn 5% of horses. Percentage of agreement between left and right front hooves and between left and right rear hooves was high (20/25 variables; % agreement > or = 99). In contrast, percentage of agreement between left front and left rear hooves and between right front and right rear hooves was low (2/25 variables; % agreement > or = 99). Presence of a pad was significantly (P < 0.05) associated with age, and several shoe variable (size, presence of a special shoe, overall wear matched) were significantly (P < 0.05) associated with sex. CLINICAL RELEVANCE: Except for variables related to special shoes, wear, and weight, 1 shoe for the respective fore- or hind limbs could be used as an indicator for the contralateral shoe worn by Thoroughbred racehorses without substantial loss of information. However, 1 shoe could not be used as an indicator for shoe characteristics of all 4 limbs. Some shoe characteristics are associated with age and sex, and these variables should be considered possible confounders in studies of shoe characteristics.
OBJECTIVE: To evaluate selected shoe characteristics as risk factors for fatal musculoskeletal injury (FMI) and specifically for suspensory apparatus failure (SAF) and cannon bone condylar fracture (CDY) of Thoroughbred racehorses in California. DESIGN: Case-control study. ANIMALS: Thoroughbred racehorses (n = 201) that died of were euthanatized at California racetracks between August 1992 and July 1994. PROCEDURE: Shoe characteristics were compared between case horses affected by FMI (155), SAF (79), and CDY (41) and control horses that died for reasons unrelated to the appendicular musculoskeletal system (non-FMI; 46). Multivariable logistic regression was used to estimate odds ratios for FMI, SAF, and CDY. RESULTS: Toe grabs were identified as possible risk factors for FMI, SAF, and CDY. The odds of FMI, SAF, and CDY were 1.8, 6.5, and 7.0, respectively, times greater for horses shod with low toe grabs than for horses shod without toe grabs on front shoes. Horses shod with regular toe grabs on front shoes had odds 3.5, 15.6, and 17.1 times greater (P < 0.05) for FMI, SAF, and CDY, respectively, compared with horses shod without toe grabs. The odds of horses shod with rim shoes were a third (P < 0.05) of those shod without rim shoes for either FMI or SAF. The apparent association between toe grab type and CDY may, in part, be attributable to concurrent SAF and CDY injuries in many horses. CLINICAL RELEVANCE: Avoiding the use of toe grabs should decrease the incidence of FMI, especially SAF, in Thoroughbred racehorses. The use of rim shoes that are more consistent with natural hoof shape may decrease injury risk.
Two groups of spirochetes were isolated from papillomatous digital dermatitis (PDD) lesions in dairy cattle. The two groups could be readily differentiated on the basis of morphologic and immunologic characteristics and enzymatic activity. A spirochete isolated from an interdigital dermatitis (IDD) lesion appeared morphologically and antigenically similar to spirochetes in one of the PDD groups and exhibited an identical enzyme activity pattern. The two groups of PDD spirochetes had characteristics most consistent with the genus Treponema. The PDD and IDD isolates differed morphologically from previously described bovine Treponema spp. Although spirochetes have been observed to be one of the predominant bacterial morphotypes in PDD and IDD and are found invading the stratum spinosum and dermal papillae in PDD lesions, the significance of these spirochetes in the etiopathogenesis of PDD and IDD is presently unknown.
Necropsies were performed on 496 horses that had a fatal injury or illness at a California racetrack during the period February 20th 1990 to March 1st 1992. The primary cause of death was categorised by breed, activity at time of injury or illness and organ system affected. Most of the submissions were Thoroughbred horses (432) and Quarter Horses (46). Most of the injuries occurred while racing (42%) and in training sessions (39%); with fewer non-exercise (12%) and accident (7%) related injuries or illnesses. Musculoskeletal injuries accounted for 83% of the Thoroughbred and 80% of the Quarter Horse submissions. The Thoroughbred horses incurred 306 fractures with 263 in the limbs and 90% of those in the forelimbs. The proximal sesamoid bone(s), third metacarpal bone and humerus were the most common bones fractured in Thoroughbred horses and Quarter Horses. Other major causes of death included respiratory, digestive and multi-organ system disorders.
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