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Biomedical subjects

D HENDLIN

Publications and source records attributed to D HENDLIN.

At least 19 recordsLinked to original sources
PubMed1965

EXCRETION AND DEGRADATION OF RIBONUCLEIC ACID BY BACILLUS SUBTILIS.

Demain, A. L. (Merck & Co., Inc., Rahway, N.J.), R. W. Burg, and D. Hendlin. Excretion and degradation of ribonucleic acid by Bacillus subtilis. J. Bacteriol. 89:640-646. 1965.-Bacillus subtilis MB-1480 was found to produce several 5'-ribonucleotides in the extracellular medium, in addition to the previously found 5'-guanosine monophosphate and guanosine diphosphate. The nucleotides accumulated after excretion of high-molecular-weight ribonucleic acid (RNA) and subsequent extracellular breakdown. Excretion of RNA paralleled growth closely, and was accompanied by deoxyribonucleic acid (DNA) and protein excretion. The process occurred in the absence of visible lysis. Whereas extracellular DNA was stable, the other macromolecules were degraded after excretion. With extracellular DNA as a measure of maximal lysis, it was calculated that the major part of the extracellular RNA must have been excreted by cells which had not undergone lysis.

Bacillus subtilis↗
PubMed1964

PRODUCTION OF EXTRACELLULAR GUANOSINE-5'-MONOPHOSPHATE BY BACILLUS SUBTILIS.

Demain, A. L. (Merck Sharp & Dohme Research Laboratories, Rahway, N.J.), I. M. Miller, and D. Hendlin. Production of extracellular guanosine-5'-monophosphate by Bacillus subtilis. J. Bacteriol. 88:991-995. 1964.-Wild-type Bacillus subtilis colonies were found to feed purineless mutants. A strain with high feeding capacity was selected for study, with a guanineless mutant of B. subtilis used as the assay organism. The factor was excreted during its growth phase in a complex medium containing starch and soybean meal extract. Nutritional studies led to the development of a defined medium to be used for biochemical studies and to aid in the isolation of the factor. Starch was replaced by maltose and the soybean meal extract by Mn(++). Production of the factor was sensitive to the pH of the medium during growth. Practically its entire extracellular accumulation occurred before visible lysis. The factor was identified as guanosine-5'-monophosphate derived by extracellular enzymatic hydrolysis of excreted ribonucleic acid.

Bacillus subtilis↗
PubMed1963

Effect of methionine, norleucine, and lysine derivatives on cephalosporin C formation in chemically defined media.

Demain, A. L. (Merck Sharp & Dohme Research Laboratories, Rahway, N.J.), Joanne F. Newkirk, and D. Hendlin. Effect of methionine, norleucine, and lysine derivatives on cephalosporin C formation in chemically defined media. J. Bacteriol. 85: 339-344. 1963.-Chemically defined media were developed for production of cephalosporin C by Cephalosporium sp. In such media, the requirement for methionine can be satisfied by norleucine. Further stimulation of antibiotic production was obtained with the lysine derivatives epsilon-N-acetyl-l-lysine and epsilon-aminocaproic acid but not with lysine itself. Also inactive were alpha-aminoadipic and ketoadipic acids. Other lysine derivatives were found to inhibit cephalosporin C production at 0.01 m. The final medium supported the production of approximately 0.5 g of cephalosporin C per liter of medium.

Acremonium↗
PubMed1962

Oxidation of glycerol by Streptococcus faecalis.

Claridge, C. A. (Merck Sharp & Dohme Research Laboratories, Rahway, N.J.) and David Hendlin. Oxidation of glycerol by Streptococcus faecalis. J. Bacteriol. 84:1181-1186. 1962.-The nature of the factors in yeast autolysate essential for the oxidation of glycerol by Streptococcus faecalis F24 was examined. Two factors appear to be involved in the oxidation of glycerol. One factor was shown to be an inducer of the enzyme system required for glycerol oxidation; the other was shown to be alpha-lipoic acid. Minute quantities of glucose will "spark" growth of S. faecalis in a medium containing glycerol and acetate as carbon sources, probably by supplying sufficient energy for induction of the glycerol-oxidation system.

Acetates↗