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Biomedical subjects

D Hamlin

Publications and source records attributed to D Hamlin.

8 recordsLinked to original sources
PubMedDate not supplied

Synthesis of 125I-labeled oligonucleotides from tributylstannylbenzamide conjugates.

A rapid and efficient method for the synthesis of 125I-labeled oligodeoxynucleotides ([125I]ODNs) is described. The key intermediates are tributylstannylbenzamide-modified ODNs (Sn-ODNs). Reaction conditions are described for the preparation of 5'-modified Sn-ODNs. Treatment with NaI and chloramine T gave conversion to the desired I-ODN, which was easily isolated by reversed phase chromatography. Thermal denaturation (Tm) studies showed that hybridization properties were not disturbed by the 4-iodobenzamide modification. An [125I]ODN was prepared and characterized by hybridization to 32P-labeled DNA targets. Sequence specific cleavage of the target DNA strand by 125I was measured.

Base Sequence↗
PubMedDate not supplied

Magnetic resonance imaging of sacrococcygeal tumors.

Five sacral tumors, 2 chordomas, 2 chondro- and/or osteosarcomas and one metastasis were examined with magnetic resonance imaging (MRI). The tumor delineation was excellent in all cases, and in several respects superior to CT. The signal pattern from the chordoma differed considerably from the other tumors suggesting a potential for tumor differentiation with MRI.

Adolescent↗
PubMedDate not supplied

Magnetic resonance imaging and tissue characterization of a renal cell carcinoma and its osseous metastasis. Report of a case.

A patient with renal cell carcinoma, first presenting because of symptoms from an osseous metastasis, was examined with CT and magnetic resonance imaging (MRI). Both the primary tumor and the metastasis were well demonstrated by both methods. MRI tissue characterization showed that the T1 values in the kidney and the metastasis were the same, as were the T2 values. This may mean a potential for the MRI to define primary tumors when a metastasis is found.

Aged↗
PubMedDate not supplied

Magnetic resonance imaging of the extremities. I. Technique for depiction of normal anatomy.

In 3 adult volunteers, the appropriate technique for depicting the normal anatomy with magnetic resonance imaging was tested. At 0.15 T, the images obtained in different anatomic planes with different pulse sequences and radiofrequency coils were compared. Spin echo technique with a repetition time of 500 ms and an echo time of 30 ms appeared as a good compromise, giving excellent depiction of both soft tissues and joints, especially if a surface coil was used. The optimum anatomic plane varied with the structures examined.

Adult↗
PubMedDate not supplied

Magnetic resonance imaging of the extremities. II. T1 and T2 relaxation times of muscle and fat. Normal values, reproducibility and dependence on physiologic variations.

In a resistive magnetic resonance imaging system, the T1 and T2 values of muscle and fat were calculated in 2 volunteers, at rest and during venous and arterial stasis, postischemic hyperemia and dehydration. The values found at rest were reproducible from one occasion to another and there were no significant differences between values in the arms and legs. Dehydration induced a slight increase in the T2, otherwise there was no change during stasis, hyperemia or dehydration.

Adipose Tissue↗