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D Hardie

Publications and source records attributed to D Hardie.

23 records · Page 2Linked to original sources
PubMed1984

Subclasses of IgG on the surface of human lymphocytes: a study with monoclonal antibodies.

Cells from sIgG+ B lymphoblastoid cell lines, sIgG+ B cell neoplasms and from tonsils, adult and cord blood and fetal spleen were tested for IgG subclass expression using a panel of monoclonal antibodies. IgG1 was in all cases the commonest subclass except on blood lymphocytes, when approximately equal numbers of cells expressing IgG1 and IgG2 were found. No evidence for multiple subclass isotype expression was found.

Adult↗
PubMed1983

Detection of free kappa chains in human serum and urine using pairs of monoclonal antibodies reacting with C kappa epitopes not available on whole immunoglobulins.

The properties of five monoclonal antibodies (McAbs) which react with free kappa chains but not with IgG kappa, IgM kappa or IgA kappa have been studied. IgG preparations stored for long periods sometimes contained low and high molecular weight material reacting as free kappa. This could be removed by passing the preparation through an affinity column to which two of the McAbs had been coupled, but reactive material reappeared when the purified IgG was heated at 65 degrees C. Two of the McAbs from one fusion reacted with F(ab')2 fragments of IgG whereas two from another fusion did not. Pairs of McAbs (one from each fusion) were much more sensitive in the detection of free kappa, particularly monomer, than a single McAb. This provided the basis for a very sensitive method of detection of free kappa chains in human body fluids and culture supernates.

Antibodies, Monoclonal↗
PubMed1982

Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity.

Thirteen monoclonal antibodies (McAbs) showing subclass-specific and subclass-restricted reactivity to human IgG have been produced by the hybridoma technique. Their specificity has been defined by passive haemagglutination (HA) and haemagglutination-inhibition (HAI) assays using a large panel pf purified IgG paraproteins of known subclass. Their use in assaying human IgG subclasses in radial immunodiffusion (RID) and in purifying human subclasses by both positive and negative immunoabsorption chromatography is illustrated.

Animals↗
PubMed1981

Properties of monoclonal antibodies to human immunoglobulin kappa and lambda chains.

Hybridomas have been produced from mice immunized with human IgG. Culture supernates were assayed for the presence of antibody-producing cells by passive haemagglutination. Hybridomas producing antibodies to human kappa (kappa) and lambda (lambda) light chains have been cloned and grown as ascitic tumours in BALB/c mice. The antigen-binding characteristics of the monoclonal antibodies, contained in the ascitic fluid, were assessed by haemagglutination inhibition, ELISA and radioimmunoassay systems and by the binding of radiolabelled antigen in analytical flat-bed iso-electric focussing gels. One monoclonal anti-kappa reacted better with free than with combined kappa chains; for another the reverse was true. Antibody fractions separated by DEAE chromatography of ascitic fluids were coupled to ox red cells with chromic chloride and compared with polyclonal antibodies for the detection of cell-surface immunoglobulins.

Animals↗