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Biomedical subjects

D Hart

Publications and source records attributed to D Hart.

At least 91 records · Page 5Linked to original sources

The psychological outcome of breast reconstruction.

My first experience with breast cancer as a nurse was in 1974. I was a 23-year-old nursing student and working as an operating room technician. A radical mastectomy was being performed on a 52-year-old woman. As I passed clamps and scissors to the two surgeons, I struggled to conceal the shock I was experiencing. "Is it really necessary to remove so much tissue for such a small lump?" "Yes," the doctor replied, "It's either this or she'll die." It was 10 years later that I would begin my work with patients undergoing breast reconstruction. During the 7-1/2 years that I worked as an operating room supervisor and assistant to a plastic surgeon, I responded to the needs of a number of women during their time of crisis. We would see each other every week or two for a year, become friends, and treatment would come to an end. Their lives would go on, and I would continue my work with a new patient, then another and another. My work with reconstruction patients continues as Patient Information Coordinator for Mentor H/S, a breast implant manufacturer. Hundreds of women who have had breast cancer call me each year. I provide information, answer questions, and help educate. Etched in my memories are emotion-filled faces and voices of shock, fear, confusion, sorrow, and resignation followed by acceptance. Individual women of various lifestyles and personalities as well as different levels of financial and social status, all have one thing in common--breast cancer, mastectomy, decisions, and reconstruction. Many were experiencing an interruption in their lives, a stress on their relationships and a drain on their finances. But the even greater challenge they faced was the life and death crisis of an illness that is both life threatening an disfiguring. Researchers such as Schain, Goldberg and Kasper have documented the psychological effects of breast-loss for women and their relationships (Schain, 1991; Goldberg, Stoltzman, & Goldberg, 1984; Kasper, 1995). Jones, Matheson, and Rowland looked at psychological adjustment, counseling needs and patients' response to their altered body image (Jones & Reznikoff, 1989; Matheson & Drever, 1990; Rowland, Holland, Chaglassian, & Kinner, 1993). The alteration of body image first occurs with mastectomy and continues with reconstruction. Goin, Cederna and Wellisch provide insight into the effects of various types of reconstruction (Goin & Goin, 1988; Cederna, Yates, Chang, Cram, & Ricciardelli, 1995; Wellisch, Schain, Noone, & Little, 1987). The following article is a compilation of my personal experience, and excerpts from literature presented as an overview of the psychological effects of mastectomy and reconstruction.

Adaptation, Psychological↗

The experience of self in the bottlenose dolphin.

Marten and Psarakos have presented some evidence which suggests that objective self-awareness and possibly representations of self may characterize the dolphins' experience of self. Their research demonstrates the possibility of similarities in the sense of self between primate species and dolphins, although whether dolphins have subjective self-awareness, personal memories, and theories of self--all important facets of the sense of self in humans--was not examined. Clearly, even this limited evidence was difficult to achieve; the difficulties in adapting methods and coding behavior are quite apparent in their report. Future progress, however, may depend upon clarification of what are the necessary components for a sense of self and an explication of how these might be reflected in dolphin behavior. We are mindful of the authors' point (pp. 219 and 220) that the dolphin lives more in an acoustic than a visual environment. Thus, while tasks relying upon vision may reveal the presence or absence of the sense of self in primates, it might well be the case that in dolphins self-related experiences might be better revealed in auditory tasks. But then, what is the nature of human self-awareness in terms of audition? While both conceptual and methodological hurdles remain, Marten and Psarakos have demonstrated that important questions can be asked about the minds and phenomenal worlds of nonanthropoid species.

Animal Communication↗

Differential macrophage responses in the peripheral and central nervous system during wallerian degeneration of axons.

We characterized quantitatively the macrophage response following axonal injury in both the peripheral (PNS) and central nervous system (CNS) of adult mammals. A monoclonal antibody (ED-1) which stains monocytes, macrophages, and activated microglia was employed. In one model, Wallerian degeneration of the sciatic nerve was studied. An increase in the number of macrophages was seen as early as 1 day following nerve transection. Macrophage number increased synchronously along the length of degenerating nerve over a 21-day period. In a second model, transection of a spinal dorsal sensory root allowed us to compare and contrast the macrophage response along the PNS and CNS portions of a single axonal pathway. An increased number of macrophages restricted to the PNS portion of this pathway was seen by 3 days and continued to increase over a 14-day period. Myelin breakdown occurred in association with an increase in the number of macrophages by 3 days in the PNS but not the CNS portion of the degenerating dorsal root axon pathway. Low-affinity nerve growth factor receptor immunohistochemical staining increased by Day 1 in the PNS but not the CNS portion of this pathway, occurring prior to the invasion of macrophages. In both models, the morphology of infiltrating macrophages changed over time from small slender ramified cells to large elongated multivacuolated cells. In conclusion, our results demonstrate that the macrophage response during Wallerian degeneration of axons in adult mammals is much more rapid and robust in the PNS, where axonal regeneration occurs, than in the CNS, where axonal regeneration is far more limited.

Animals↗

Women and saline breast implant surgery.

Since clinical studies are ongoing relative to breast implants, keeping up with the most current information is an important aspect of nursing practice. This article will provide answers to five basic questions that arise in regards to breast implantation and nurses' roles in the process.

Breast Implants↗

Measuring disadvantage: changes in the underprivileged area, Townsend, and Carstairs scores 1981-91.

OBJECTIVE: To compare the intercensal change for each of the underprivileged area (UPA), Townsend, and Carstairs scores calculated from 1981 and 1991 census data. SETTING: England and Wales. METHODS: The method described enables comparison of change in composite scores such as the UPA, Townsend, and Carstairs scores which are derived from normalised variables. The national values of equivalent variables derived from the censuses are calculated and normalised on the same baseline of the 1981 electoral ward mean and SD values. The resultant change in composite scores for different censuses can then be compared directly. MAIN OUTCOME MEASURE: Change in the composite score values for the 1991 census when compared with the 1981 census. RESULTS: For England and Wales, the UPA score increased by 5.62 units (0.35 of the SD) but the Townsend and Carstairs scores fell by 2.39 and 1.13 units respectively (0.71 and 0.33 of the SDs). CONCLUSION: The Townsend and Carstairs scores are good measures of material deprivation and show a general improvement as such between 1981 and 1991. The UPA score, however, includes additional factors relating to family structure, social deprivation, and health need and shows a decline in the overall situation.

Data Interpretation, Statistical↗

Technical note: potentially higher patient radiation doses using digital equipment for barium studies.

Digital fluorography offers several advantages over conventional radiographic systems. The general expectation of digital equipment has been of a lower radiation dose to the patient. A series of dose-area product measurements on two digital sets during barium meal and barium enema examinations has shown that this expectation is not necessarily correct, particularly when the dose from the fluoroscopic part of the examination is considered. Users of digital fluorography sets are advised to check the performance of their equipment by suitable dose measurements.

Barium Sulfate↗

Prosocial behavior and caring in adolescence: relations to self-understanding and social judgment.

The relation of self-understanding and moral judgment to dedicated prosocial behavior is investigated. Participants were African-American and Latin-American adolescents who had been nominated by community leaders for having demonstrated unusual commitments to care for others or the community (care exemplars). The care exemplars, and matched comparison adolescents, were extensively interviewed over the course of 4-6 sessions in order to elicit self-understanding, moral judgment, and implicit personality theories. The care exemplars were more likely than the comparison adolescents to: (1) describe themselves in terms of moral personality traits and goals, (2) view themselves as having closer continuity to their pasts and futures, (3) think of themselves as incorporating their ideals and parental images, and (4) articulate theories of self in which personal beliefs and philosophies are important. There were no differences between the care exemplars and the comparison adolescents in developmental stages of moral judgment nor in the abstractness of their implicit personality theories.

Adolescent↗

The plasma interleukin-6 and stress hormone responses to acute pyelonephritis.

The relationships between the "stress hormones" corticotrophin (ACTH), vasopressin (AVP), corticotrophin releasing hormone (CRH) and cortisol, and the cytokines, interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor were studied during an acute infection. Ten patients (7 female, 3 male, age range 16-56 years) with acute pyelonephritis and normal renal function were studied during the first 72 hours following hospital admission. Peptide hormones were measured by radioimmunoassay, cortisol and cytokines by ELISA. Reference ranges for all hormones were from samples donated by 40 or more volunteers from the electoral roll. The reference data for IL-6 was obtained from 20 normal donor sera. The mean plasma IL-6, AVP and CRH concentrations on admission to hospital were significantly raised above the mean 08:00h values of the normal volunteers (p < 0.001 for AVP and CRH, p < 0.01 for IL-6), but mean plasma ACTH and cortisol were not. Mean plasma IL-6 and AVP were raised more than two standard deviations above the mean of the reference range for 72 hours, although IL-6 tended to fall after 24 hours. No change in plasma IL-1 and tumour necrosis factor was observed in three patients. The correlation between plasma IL-6 and cortisol concentrations at all sampling times and in all subjects was highly significant (p < 0.001). Significant correlations between plasma IL-6 and AVP (p < 0.005), and IL-6 and ACTH (p < 0.05) were also observed. No correlation between IL-6 and CRH could be demonstrated.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute Disease↗

Educational audit of postgraduate medical education.

This project examined the academic audit of postgraduate medical education, and went on to research, design and implement an educational audit system for use in Mersey Region, UK. Issues found to be significant included ownership of both standards and results, norming, and the levels of aggregation of data. The project has demonstrated that designing and implementing a system of feedback which is effective and sustainable is feasible, but requires the agreement and support of tutors as to its objectives and use. The resulting system has now been tested across a range of courses and programmes of meetings in Mersey Region, and has been accepted by the Postgraduate Office as the basis for future development.

Education, Medical, Graduate↗

Defining osteoarthritis of the hand for epidemiological studies: the Chingford Study.

OBJECTIVES: To explore the relative merits of clinical and radiological examination in defining hand osteoarthritis (OA) for epidemiological purposes. METHODS: A total of 976 women aged 45-64 years were selected from the general population and underwent a structured interview, clinical examination of the hand joints and radiography of the hands and knees. The inter-relationship of the clinical and radiological findings was examined, and also the association of each with hand symptoms and the presence of knee OA. RESULTS: Clinical and radiographic signs of hand OA generally correlated with each other, and each was associated with hand symptoms to a similar degree. When analysed in combination, they were only marginally better at predicting symptoms than when tested individually. However, when they were examined in relation to radiographic features of knee OA, there was a significantly stronger association with radiographic features of hand OA than with clinical features. CONCLUSIONS: Hand radiology provides a better overall assessment of osteoarthritis than physical examination of the interphalangeal joints or carpo-metacarpal joint; in situations where radiology is not available clinical examination is a viable substitute.

England↗

Mechanism of endothelin activation of phospholipase A2 in rat renal medullary interstitial cells.

Previous studies from this laboratory have demonstrated that endothelin-1 (ET) stimulates phosphatidylinositol (PI) hydrolysis, activates dihydropyridine-insensitive Ca2+ channels, and promotes prostaglandin E2 (PGE2) accumulation in cultured rat renal medullary interstitial cells (RMIC). The mechanism whereby ET augments PGE2 production was explored in the current study. ET-evoked PGE2 accumulation proceeded independent of large increments in cytosolic free Ca2+ concentration ([Ca2+]i), derived from either extracellular or intracellular sources. Chelation of intracellular Ca2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid eliminated ET-evoked PGE2 production, indicating that eicosanoid production was nonetheless a Ca(2+)-requiring process. Nanomolar concentrations of phorbol 12-myristate 13-acetate (PMA) alone did not stimulate PGE2 production, nor did PMA alter ET-stimulated PGE2 accumulation. Furthermore, downregulation of protein kinase C (PKC) by prolonged exposure of cells to PMA did not mitigate ET-mediated PGE2 production, demonstrating that PKC stimulation was not required for PGE2 production. ET stimulated PGE2 accumulation despite PI-specific phospholipase C (PI-PLC) inhibition by nanomolar concentrations of PMA, indicating that eicosanoid production was not a downstream event of PI hydrolysis. ET stimulated arachidonic acid metabolite release in parallel with a loss of label from membrane phospholipids. Phosphatidylethanolamine was the preferred substrate for ET-mediated activation of phospholipase A2 (PLA2). Immunocytochemical studies including immunostaining, immunoblotting, and immunoprecipitation confirmed the presence of cytosolic PLA2 (cPLA2) in RMIC. In summary, ET stimulation of PGE2 production in RMIC is mediated via agonist activation of cPLA2 independent of activation of PI-PLC, suggesting direct coupling to the ET receptor. Constitutive levels of [Ca2+]i rather than abrupt increments in [Ca2+]i are sufficient for activation of this receptor-effector system, with no obligatory requirement for PKC.

Animals↗

Estimation of effective dose from dose-area product measurements for barium meals and barium enemas.

The International Commission on Radiological Protection has recommended the use of a new dose quantity "effective dose". The doses to 22 organs of the human body are required for a formal calculation of this quantity. This paper shows that a quick estimate of the effective dose received by a patient from a barium meal or a barium enema can be made by multiplying the measured dose-area product for the complete examination by an appropriate conversion coefficient. Despite the varied techniques used for barium examinations, such an estimate is unlikely to be in error by more than 25%.

Administration, Oral↗

Patient radiation doses from enteroclysis examinations.

Data relating to patient dose have been acquired for enteroclysis examinations (small bowel enemas) performed at the John Radcliffe Hospital, Oxford, on 23 adult patients. Dose-area products, fluoroscopy times and the number of radiographs taken are used to compare the examination procedure at the John Radcliffe Hospital with enteroclysis and barium follow-throughs performed elsewhere. The mean dose-area product for the 23 examinations was 6.8 Gy cm2 and the mean effective dose was estimated to be 1.5 mSv. These doses are intermediate between those arising from barium meals and barium enemas performed in the same room.

Adult↗

Self-reported fears of hospitalized school-age children.

This research examines the fears of 82 hospitalized school-age children 8 to 11 years of age as self-reported on the Child Medical Fear Scale (CMFS) (Broome, Hellier, Wilson, Dale, & Glanville, 1988). The variables of age, gender, yearly income level, health status (acute or chronic), and the score on the Trait scale of the State-Trait Anxiety Inventory for Children (STAIC), (Spielberger, Edwards, Lushene, Montuori & Platzek, 1973) were examined. Fear of separation from the family, having shots and finger sticks, having to stay in the hospital for a long time, and being told that something was wrong with them were the fears with highest mean scores. Children with higher trait anxiety and from families with lower yearly income reported a higher amount of fear. Pediatric nurses are encouraged to perform fear assessments and provide individualized interventions that anticipate fear reactions in order to assist school-age children to cope with sources of fears during hospitalization.

Adaptation, Psychological↗

Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway: implications for the possible role of dietary fibre in the prevention of large-bowel cancer.

The purpose of this study was to determine whether cultured colonic adenoma and carcinoma cells undergo apoptosis (programmed cell death) in vitro and whether specific growth and dietary factors, thought to be involved in the control of growth and differentiation of human colonic cells, could induce cell death through apoptosis. In cell lines originating from 6 colorectal adenomas and 7 carcinomas, spontaneous apoptosis was observed. Sodium butyrate, a naturally occurring fatty acid, is present in the human large bowel in millimolar amounts as a result of bacterial fermentation of dietary fibre. Sodium butyrate, at physiological concentrations, induced apoptosis in 2 adenoma cell lines, RG/C2 and AA/Cl, and in the carcinoma cell line PC/JW/FI. In contrast, transforming growth factor beta 1, which is thought to have an important role in the control of growth in colonic epithelium, did not induce apoptosis. Neither RG/C2 nor PC/JW/FI contain wild-type p53, therefore this tumour-suppressor gene is not required to mediate signals for the induction of apoptosis in colonic tumour cells. Our studies report the induction of apoptosis in colonic tumour cells by the naturally occurring fatty acid sodium butyrate. Since sodium butyrate is produced by bacterial fermentation of dietary fibre, the observation that this fatty acid can induce apoptosis could, in part, explain why a high-fibre diet appears to be protective against colon cancer. Escape from the induction of programmed cell death may be an important event in colorectal carcinogenesis.

Adenoma↗

Response of plasma low density lipoprotein subfractions to oestrogen replacement therapy following surgical menopause.

OBJECTIVE: Epidemiological studies suggest that postmenopausal oestrogen replacement reduces the incidence of cardiovascular disease. The purpose of this study was to establish the effects of oestrogen replacement therapy on subfractions of plasma low density lipoprotein in bilaterally oophorectomized women. DESIGN: In a placebo controlled, double-blind study, patients were randomized on a two to one basis to receive either oestradiol valerate (2 mg/day) or placebo respectively for a period of 16 weeks. PATIENTS: Seventeen women aged 28-51 years who had all had hysterectomy and bilateral oophorectomy at least 2 months before recruitment were assigned to either the active (n = 12) or placebo (n = 5) group. MEASUREMENTS: Plasma lipids, lipoproteins, apolipoproteins and LDL subfractions were determined immediately before and after the treatment period. LDL subfractions were isolated directly from plasma by density gradient ultracentrifugation within 24 hours. Non-parametric statistical analysis was carried out within each group using Wilcoxon's signed rank test for matched pairs. RESULTS: After 16 weeks of treatment, HDL cholesterol, apo A-I and HDL-2 were increased in the group receiving oestrogen (HDL cholesterol +12%, P < 0.01; apo A-I +14%, P < 0.01; HDL-2 +24% P < 0.01). While there were no significant changes in serum cholesterol, LDL cholesterol or triglycerides, the proportion and concentration of the least dense LDL-I subfraction was decreased significantly (-27%, P < 0.05). The LDL subfraction of intermediate density (LDL-II) was decreased in eight subjects, while small, dense LDL-III was unaffected. Overall, these changes resulted in an apparent shift in the distribution of LDL subfractions towards small, dense LDL-III, although there was no net increase in the latter. CONCLUSION: In view of a similar and characteristic response of LDL subfractions to hypolipidaemic drugs that enhance the clearance of LDL via the LDL receptor, the present findings suggest that oestrogen promotes the preferential removal of LDL-I and II by activating LDL receptors. As this effect is normally associated with a reduction in the circulating level of LDL, it should not be regarded as an unfavourable response to oestrogen replacement therapy.

Adult↗

Bond strength of composite to alloy treated with bonding systems.

PURPOSE: The in-vitro bond strengths of a composite bonded to a nickel-chromium-beryllium alloy treated by eight bonding systems were measured after three storage conditions. MATERIALS AND METHODS: Nickel-chromium-beryllium alloy samples were treated by eight commercial bonding systems including adhesive composite cements, all-purpose bonding agents, and silica-coating systems. A composite was bonded to the alloy samples. Sample groups were stored in water for 24 hours at 23 degrees C, or thermocycled, or stored for 6 months at 23 degrees C and then debonded in tension. RESULTS: Bond strengths after 24 hours storage were: adhesive composite cements, 14.2 to 22.1 MPa; all-purpose bonding agents, 11.4 to 14.6 MPa; and silica-coating systems, 18.6 to 20.2 MPa. Bond strengths after thermocycling were: adhesive composite cements, 12.6 to 20.6 MPa; all-purpose bonding agents, 9.9 to 17.7 MPa; and silica-coating systems, 11.1 to 19.2 MPa. Bond strengths after 6 months were: adhesive composite cements, 12.0 to 13.1 MPa; all-purpose bonding agents, 8.7 to 14.1 MPa; and silica-coating systems, 14.8 to 18.4 MPa. CONCLUSIONS: Only two bonding systems showed decreased bond strength after thermocycling, as compared with 24 hours' storage; whereas four bond systems showed decreased bond strength after 6 months' storage. Bond strengths measured after thermocycling were not predictive of those measured after 6 months' storage.

Analysis of Variance↗