Biomedical subjects

D Heitmann

Publications and source records attributed to D Heitmann.

76 records · Page 5Linked to original sources

PubMed1977

[The diagnostic value of mammography during pregnancy and lactation (the radiological features of the breast during pregnancy) (author's transl)].

During early and late pregnancy and lactation, there is an increase in the size of the breasts, with increased density of their structure. The moderately dense parenchyma in young women is characterised by a) wavy contours of the subcutaneous zone and b) linear or oval translucencies in the central parenchyma. Our investigations indicate that it usually takes three months after delivery before the breast structure returns to normal and one is able to interpret the mammogram satisfactorily. For this reason mammography should be avoided during these three months and if there is a suspicion of disease in the breast, a biopsy should be carried out immediately.

Adolescent↗

PubMed1977

[Life expectancy in penile carcinoma (statistical evaluation of 150 penile carcinomas in the period 1912-1970)].

In the period 1912-1970 in the Göttingen area, 150 patients suffering from penis carcinoma were investigated and/or treated. With regard to method of treatment, three periods can be distinguished: 1912-1930, 1931-1948, and 1949-1970. During the first period, treatment was chiefly radiotherapy, in the second chiefly surgery, and in the third combined radiotherapy and surgery. Independent of the type of therapy and period of observation, the 3-, 5-, and 10-year survival rates were 52.5%, 46.7%, and 31.4%, respectively. The greatest life expectancy was that following combined surgical and high-voltage therapy. The importance of lymphography is emphasized.

Adult↗

PubMed1977

[Carcinoma of the penis: symptomatics, therapy and prognosis (author's transl)].

During the period from 1912 through 1970, 150 cases with a carcinoma of the penis have been examined and treated in the clinics of the university of Göttingen. The preferred age of incidence was between fifty to sixty years. Primary phimosis could be verified in 17.3%, a secondary phimosis, caused by the tumor, in 5.3%. Eighty patients were graded stage I, seventy stage II and more advanced stages. Metastases to the inguinal region were observed in 60.6% out of 99 patients who had undergone lymphadenectomy. Distinctly enlarged inguinal lymph nodes proved to be metastases in only a third of the cases. The staging of a case with carcinoma of the penis, therefore, is not possible by means of clinical criteria alone but needs also a biopsy from the inguinal region. The 3-year, 5-year, and 10-year survival rates amounted to 52.5%, 46.7% and 32.4% and did not depend on treatment techniques. The most promising survival rate resulted from a combination of surgical treatment with high-voltage therapy.

Adult↗

PubMedDate not supplied

Replacement of chloroform throughout glycosphingolipid isolation.

Chloroform, the predominant constituent of solvents used for lipid extraction and chromatography, is believed to give rise to birth defects and serious damage to health, and may also be carcinogenic. Therefore, simple and successful methods have been developed to replace chloroform throughout the isolation of glycosphingolipids (GSLs) by less harmful solvents. Gangliosides of sheep brain (ganglio-series gangliosides GM1, GDla, GD1b and GT1b) and of lymphocyte-derived mouse hybridoma cells (namely GM3) were extracted with six different solvent mixtures. Chloroform:methanol:water (40:80:30, v/v/v) was employed as reference (solvent I). Combinations without chloroform were: n-propanol:water (40:10, v/v) (II), methylisobutylketone:methanol:water (40:80:30, v/v/v) (III), ethylacetate:methanol:water (40:72:28, v/v/v) (IV), methylacetate:methanol:water (40:72:28, v/v/v) (V) and petroleum ether:isopropanol:water (40:112:38, v/v/v) (VI). After extraction and dialysis, the weight of lipid extract as well as the content of sialic acid, gangliosides, sulphatides and phospholipids were determined. Quantitation of GSL yields in crude extracts obtained by the alternative solvent mixtures II to VI showed recoveries of brain gangliosides from nearly 67% up to 104% compared with the reference solvent I. Extraction of hybridoma cells by means of the alternative combinations without chloroform revealed at least the same and mostly better ganglioside yields in the range from 98% to 116% with regard to the reference solvent I. n-Propanol:water (II) and methylisobutylketone:methanol:water (III) were the recommended extractants for both tissues. Therefore, the methods described offer simple, less hazardous and successful strategies for GSL extraction in excellent yield without the need for using chloroform.

Animals↗