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Biomedical subjects

D Hollard

Publications and source records attributed to D Hollard.

At least 55 records · Page 3Linked to original sources
PubMed1979

Partial characterization of colony stimulating activity from human leucocyte conditioned medium tested on human marrow cells.

Conditioned medium from normal human leucocytes was fractionated by gel filtration (Sephadex G 150) and affinity chromatography (Con A-Sepharose). On gel filtration, all colony stimulating activity (CSA) was eluted as a single peak with a molecular weight of 33.000 -- 41.000. Affinity chromatography revealed two separate peaks of colony stimulating activity, one lacking and the other having concanavalin A affinity (MW congruent to 44.000 -- 58.000). The properties of these two fractions are under investigation.

Bone Marrow Cells↗
PubMed1979

[Plasma cell beta-glucuronidase and monoclonal gammapathies (author's transl)].

beta-glucuronidase activity, estimated cytochemically, is, in more than half cases of myeloma, very increased or decreased, compared to that found in benign dysglobulinemias, where this activity is normal. A very high or very low score makes sure the diagnostic of myeloma. The relations between the beta-glucuronidase activity and the myeloma clinical stade according to Durie and Salmon and the bone lysis are discussed.

Clinical Enzyme Tests↗
PubMed1979

In vitro granulopoiesis in oligoblastic leukaemia: prognostic value, characterization and serial cloning of bone marrow colony and cluster forming cells in agar culture.

The colony and cluster forming capacity of bone marrow cells (BM CFC and CluFC) in agar culture, was studied from 20 oligoblastic patients. 13 patients had a leukemic growth pattern and 12 of these died within one year after diagnosis. 7 patients had no leukemic growth and 4 are alive 16 to 90 months after diagnosis. Both the determination of the proportion of abnormally light buoyant density of CFC and CluFC and the study of their suiciding index were used to characterize more precisely the leukemic or nonleukemic status of patients. Because of the small number of patients involved in the later preliminary study, the prognostic significance cannot be valuated. Serial studies of individual patients showed different types of evolution in the growth pattern of BM CFC and CluFC. Either the increase of BM CFC and CluFC paralleled that of the myeloblasts, or there was a change in the growth pattern before AML transformation suggesting clonal evolution.

Aged↗
PubMed1977

The proteolytic action of bacterial proteases from Clostridium histolyticum on bovine fibrinogen.

Bacterial proteases from Clostridium histolyticum bring about the polymerization of fibrinogen into structured fibres, the period being 9 nm when the ionic strength (I) of the solution is between 0.1 and 0.2. At I=0.3 no polymerization occurs, but the successive actions of bacterial proteases and thrombin induce polymerization, the fibres obtained showing a periodic structure: the major period is 23 nm with two minor striations. The striation pattern is different from that obtained with fibrin which shows a major period of 23 nm with three minor striations. The degree of degradation of fibrinogen monitored by disc gel electrophoresis shows that the Aalpha polypeptide chain (mol. wt.=68 000) is degraded into Aalpha1 (mol. wt.=32 000) and Aalpha2 (mol. wt.=29 000), whilst the mobility of Bbeta increases and gamma remains unchanged; the molecular weight is estimated between 272 000 and 269 000. These results emphasize that the charge distribution differences which are compatible with the loss of different portions of the molecule, lead to variations in fibre structures.

Animals↗
PubMed1977

Quantitative study of T and B lymphocytes in Hodgkin's disease.

Peripheral blood lymphocytes forming E rosettes in the presence of sheep red blood cells and those bearing surface immunoglobulins (SIg) have been studied quantitatively as an evaluation of T and B lymphocytes in Hodgkin's disease. 62 patients were investigated, 21 of whom before any treatment. It appears that the lymphocytes forming E rosettes are significantly lower in percentage in 86% of the patients and in absolute count in 65%. SIg bearing lymphocytes are elevated in percentile in 61% of the cases, but the absolute count is normal in 50% of the patients and elevated in 30% only. At diagnosis the T/B lymphocytes equilibrium is modified in 13 among 21 patients but, after the initial treatment of the disease, the ratio is modified in 90% of the patients in complete remission and remains unchanged for years even in the absence of relapse or immnunosuppressive treatment. It is suggested that SIg + lymphocytes from the peripheral blood are actually B lymphocytes and not anti-T-antibody coated T lymphocytes or antigen-antibody lacking of membrane markers, which are numerous in one third of the investigated patients, might be T lymphocytes with qualitative abnormality.

Adult↗
PubMed1976

[Trial of androgen therapy in the treatment of non-lymphoblastic acute leukemia. First results].

Addition of a daily dose of androgen in the form of 0.15 mg/kg of Stanzolol, given without interruption, gave an average survival of more than 4 years in patients suffering from granulocyte series acute leukaemias after complete remission was obtained. The simplicity of this treatment is apparent only from the appearance of marked manifestations of androgen impregnation in women from the 8th month of treatment onwards. These results, superior to those obtained up to the present time in the survival of myeloid leukaemias (non-lymphoblastic) were also better in terms of the stability and X "quality" of the remission in comparison to those obtained in acute lymphoblastic leukaemias. Confirmation of these results by controlled clinical trial will open up interesting perspectives, along side immunotherapy which remains of unproven effectiveness in myeloid leukaemias. The effectiveness of androgen stimulation of haematopoiesis as a stabilising factor of complete remissions in acute leukaemias has, in addition, interesting implications with regard to the theory of the leukaemic process.

Age Factors↗
PubMed1975

[Granulopoiesis and its regulation].

The neutrophil granulocytes are derived, in the present state of our knowledge, from a hemocytoblast identical with that of the other blood cells, defined by its power to form in vivo clones of multiple composition, defining their characteristic of totipotential undifferentiated cell. The way in which the clone-forming cell evolves in vivo towards granulopoiesis, depends on a genetic factor, phenomena of derepression and an extrinsic factor, the origin of which is probably cellular. Differentiation of the hemocytoblast leads to a cell, the destiny of which is then fixed, which may then form clones in vitro in semi-solid medium (clone-forming cell in vitro or CFC). This cell is definitely a true entity. A humoral factor of macrophage origin intervenes to ensure granulopoiesis in vivo (clone-stimulating factor CSF3. The phenomena which regulate this granulopoiesis then cause to intervene various biochemical forms of this clone-stimulating factor and an inhibitor, the reality of which is not yet definitely demonstrated in vivo. Laboratory animals and cell culture of human normal and leukemic granular cells now supplies experimental models which permit studies of the granular differentiation of the hemocytoblast completed by kinetic studies in vivo to make rapid progress, especially in our knowledge of the human leukemic process.

Anemia, Aplastic↗