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Biomedical subjects

D Hsu

Publications and source records attributed to D Hsu.

59 records · Page 4Linked to original sources
PubMedDate not supplied

Human cell cultures infected by tumor-inducing and non-tumor-inducing viruses, an electron microscopic study.

This study is dealing with the infection patterns of adenovirus type 2 and type 12 on human fibroblast cell lines, KB, WI and MAF. With the exception of Ad -12 leads to WI, many intranuclear viral particles were present. None of these second passages (Ad-2 WI-WI, Ad-12 WI-WI, Ad-2 MAF-MAF, Ad-12 MAF-MAF) was found to have viral production. This indicates that the injections on both WI and MAF cells caused by both Ad-2 and Ad-12 cannot be serially transmitted. However, when these infected cells were used to expose KB cells, significant viral yields were obtained. This shows that the infected cells might still carry the viral specific antigens although no visible virions were observed.

Adenoviridae↗
PubMedDate not supplied

Bifunctional adsorbents for hydrophobic displacement chromatography of proteins.

The separation of pancreatic trypsinogen and alpha-chymochypsinogen A by displacement chromatography was tested on a bifunctional adsorbent containing both butyl and carboxymethyl groups. Methacrylic triblock copolymer was synthesized and used as the displacer. Compared to the displacement results on commercial Butyl-Sepharose, it was found that both the separation and recovery of trypsinogen and alpha-chymochypsinogen A were improved. Adsorption isotherms of proteins were measured on both the commercial Butyl-Sepharose and the synthesized bifunctional adsorbents. It was found that the improvement of protein separation on bifunctional adsorbents was attributed to the alteration of the adsorption of trypsinogen. Charge repulsion between trypsinogen and the negatively charged carboxymethyl groups may account for the alteration. In addition, taking advantage of the effect of charge repulsion, the column regeneration became much easier on the column packed with bifunctional adsorbents.

Chromatography, Liquid↗
PubMedDate not supplied

Pediatric retransplantation.

Previous studies have attempted to outline the efficacy of heart retransplantation in adults. A limited number of these retransplantation procedures have been performed in children; however, no study to date has evaluated the risk of heart retransplantation in this specific patient population. We conducted a retrospective review of 17 pediatric (non-neonatal) heart transplant recipients who subsequently underwent heart retransplantation. Thirteen male and four female patients underwent retransplantation at four different institutions between 1974 and 1992. Patient age at the time of primary transplantation ranged from 2 to 19 years (mean, 12.5 years) and from 3 to 30 years (mean, 16 years) at retransplantation. The time interval between transplantation procedures ranged from 2 days to 15.6 years and was not predictive of patient outcome. Follow-up after retransplantation ranged from 1 day to 11 years (mean, 2.8 years). All patients were New York Heart Association class 3 or class 4 at the time of retransplantation. After retransplantation all survivors were New York Heart Association class 1. No patient had mechanical device implantation or extracorporeal membrane oxygenation bridge-to-transplantation at primary transplantation or retransplantation. The indication for retransplantation was transplant coronary artery disease (TxCAD) in seven patients (41.2%), acute rejection in four (23.5%), chronic rejection in one (5.8%), chronic rejection associated with TxCAD in four (23.5%), and intraoperative donor organ failure in one (5.8%). No significant difference occurred in linearized rates of rejection or infection or in actuarial freedom from rejection or infection when secondary grafts were compared with primary grafts in patients undergoing retransplantation.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗