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Biomedical subjects

D Hu

Publications and source records attributed to D Hu.

At least 127 records · Page 7Linked to original sources
PubMed1997

[Efficacy of a triclosan/copolymer dentifrice in the control of plaque and gingivitis: a six-month study in China].

A total of one hundred fifty three healthy male and female adults were entered into a six month, double-blind clinical study designed to evaluate the effect of a dentifrice containing 0.3% triclosan and 2.0% PVM/MA copolymer in a 0.243% sodium fluoride/silica base, relative to a 0.243% sodium fluoride silica base control dentifrice on supragingival plaque accumulation and gingivitis. Plaque and gingivitis were scored after 3 and 6 months' use of the assigned dentifrice. At the end of the study, the dentifrice containing triclosan/copolymer demonstrated an average reduction, relative to the control dentifrice of 16.1% in plaque accumulation and 24.3% in gingivitis. Both were statistically significant(P < 0.001). It was concluded that the twice-daily use of the triclosan/copolymer containing dentifrice resulted in a significant reduction in plaque formation and a significant improvement in gingival health. No side-effects were observed.

Adult↗
PubMed1997

[Clinical study on the antithrombotic effects of Holothuria ldeucospilota acidic mucopolysaccharide].

OBJECTIVE: To study the antithrombotic effects of Holothuria Ldeucospilota Acidic Mucopolysaccharide (HLAM). METHODS: A series of variables were observed before and after treatment with HLAM in normal middle-aged subjects and patients recovering from cerebral embolism or suffering from ischemic heart disease, each of which were divided into two groups according to the dosage administered (60 mg/d and 120 mg/d). RESULTS: The fat metabolism was enhanced in addition to the anticoagulant and the blood viscosity reducing effects. No side-effect was found. CONCLUSION: HLAM is a promising drug for antithrombotic therapy.

Adult↗
PubMed1997

Expression and cellular location of endothelin-1 mRNA in rat liver following endotoxemia.

OBJECTIVE: To study the effect of endotoxin on the alteration of the transcription, expression and cellular location of endothelin-1 (ET-1) mRNA in the hepatic tissue. METHODS: Wistar rats were divided into control and endotoxic group. The rats in the control group were injected with saline, and those in the endotoxic group with endotoxin at a dose of 10 mg.kg-1 body wt. ET-1 hepatic homogenate was assayed by radioimmunoassay at 3, 6, 9, 12 and 24 h after endotoxin administration. Dot blot was used to identify and quantify ET-1 mRNA of the hepatic tissue. Hybridization of ET-1 of the hepatic tissue was proceeded at 3, 6, 12 and 24 h after endotoxin administration. RESULTS: ET-1 concentrations and the level of ET-1 mRNA increased rapidly and reached the peak at 6 h, and remained high at 24 h after endotoxin administration. By in situ hybridization, ET-1 mRNA was found in hepatic sinusoids, endothelial cells of portal vein and kupffer cells. CONCLUSIONS: Endotoxin may be the principal stimulating factor for expression of ET-1 mRNA in hepatic tissue. Endotoxin may affect transcription and translation level of ET-1, leading to increase of the synthesis and release of ET-1. The hepatic vascular endothelial cells, hepatic sinusoids and Kupffer cells all synthesize and release ET-1.

Animals↗
PubMed1997

Multicenter clinical trial of thrombolytic therapy in 1,406 patients with acute myocardial infarction. Collaborative Group of Clinical Trial for Urokinase Therapy.

OBJECTIVE: To investigate the clinical efficacy and safety of intravenous thrombolytic therapy of Urokinase Tech-Pool (UKTP) in acute myocardial infarction (AMI). METHODS: The data were collected from the 148 participating hospitals from November, 1994 to April, 1996. A total of 1,406 patients with AMI were analyzed to evaluate the clinical efficacy, side effects and mortality of UKTP. The patency of the infarct-related artery (IRA) was evaluated in 124 patients by coronary artery angiography (CAG) 90 minutes after the onset of UKTP infusion. RESULTS: The reperfusion rate in IRA was 73.5% by clinical standards. The patency rate was 72.6% by CAG. The total mortality during the first 5 weeks was 7.8% (109/1,406). The rate of minor bleeding was 10.2% (143/1,406), of major bleeding 0.43% (6/1,406) and of intracranial hemorrhage 0.50% (7/1,406). In elderly patients (> 75 years old), UKTP was as effective and safe as in younger patients (< 65 years old). Late thrombolytic therapy with UKTP (> 6 hours after the onset of symptom) was still effective. The appropriate dosage of UKTP might be 150 million units of infusion within 30 minutes. CONCLUSION: UKTP is an effective, reliable and safe agent in the thrombolytic therapy of AMI.

Aged↗
PubMed1996

[Arterioportal fistulas in the extra-hepatic circulation of the common hepatic artery. Diagnosis and radiologic interventional therapy].

Two cases of arterioportal fistulas and new possibilities in diagnosis and treatment are reported: A 62-year-old women was admitted to our hospital with abdominal pain. Color flow duplex sonography showed an arterioportal fistula between the proper hepatic artery and the ectatic portal vein that was confirmed by angiography, permitting percutaneous embolization as a definitive treatment. The pain disappeared immediately. A 36-year-old man underwent surgery due to a penetrating gastric ulcer. He was referred to us with an abdominal murmur. Color flow duplex sonography proved by spiral CT with 3D reconstruction and maximum intensity projection (angio-CT) revealed an arteriovenous fistula between the gastroduodenal artery and a mesenteric branch. Angiography confirmed the diagnosis and permitted definitive therapeutic embolization.

Adult↗
PubMed1996

Changes in substance P in the jejuna of rats after burns.

Radioimmunoassay (RIA) was used to determine the dynamic changes of immunoreactive substance P (iSP) in the jejuna of rats (TBSA 30 per cent full skin thickness burn) during the first 72 h postburn. Immunohistochemistry and image analysis techniques were used to observe and quantitate the SP immunoreactivity (SP-IR) of positive nerve fibres in the villi of jejuna postburn. Changes in the amount of iSP in jejuna of burned rats were: (1) iSP increased significantly at 1 h postburn, and the high level of iSP lasted 4 h; it then decreased greatly 8 h later with the low level of iSP persisting for 72 h. (2) Significant changes in SP-IR-positive nerve fibres in the villi after burn were shown by the immunohistochemical studies including the morphoses; the distributive densities and SP-IR-positive products in the SP-IR-positive nerve fibres. The results quantified by image analysis showed similar alterations in the distributive densities and SP-IR-positive products in the nerve fibres in the villi during 72 h postburn; which were distinctly elevated by 1 hr then dropped by 8 h and 12 h and finally elevated again. The results indicated that the irritation-release and consumption of SP occurred in jejuna of rats after burns. It might be that SP contributed to the postburn intestinal lesion in rats by bioactivities, such as enhancing the vascular permeability and regulating the intestinal movement. The SP peptidergic nerve fibres of the villus had a direct effect on the damage to mucosal epithelia.

Animals↗
PubMed1996

Mouse lethal activity of a HEp-2 vacuolation factor, cereulide, produced by Bacillus cereus isolated from vomiting-type food poisoning.

The HEp-2 vacuolation factor (or cereulide) produced by Bacillus cereus isolated from vomiting-type food poisoning, which is supposed to induce emesis, was found to give mouse and suncus lethality after intravenous and intraperitoneal administration. The emetic activity of the factor was also found to be resistant to heating at 121 degrees C for 15 min, exposure to pH 2 and 11, and to digestion with proteolytic enzymes such as pepsin and trypsin. These findings suggest that the cereulide produced by B. cereus is stable in the digestive tracts, induce emesis, and show lethal activity leading to cellular damage.

Animals↗
PubMed1996

[Effect of dl-3-n-butylphthalide (NBP) on purine metabolites in striatum extracellular fluid in four-vessel occlusion rats].

The effects of NBP on concentrations of some purine metabolites in extracellular fluid of rat striatum during global ischemia and reperfusion were studied. Global ischemia was produced by the four-vessel occlusion method. Push-pull cannula was implanted stereotaxically into the striatum of rat and was perfused with Ringer's solution at a flow rate of 2.5 microliters.min-1. The level of adenosine(Ade), inosine(Ino), hypoxanthine(Hyp) and xanthine(Xan) in perfusates were measured with HPLC connected with a UV detector. The results indicate that the levels of ade, ino, hyp and xan were significantly increased (about 3-5 times of initial value) during cerebral ischemia and reperfusion. NBP at the dose of 20 or 40 mg.kg-1 given intra-peritoneally 20 min before ischemia was shown to depress the increase of ade, ino, hyp and xan during ischemia and reperfusion dose dependently. But no change in the level of purine metabolites was found in sham operated rats. It has been known that harmful free radicals were produced when xan and uric acid were formed by xanthine oxidase during reperfusion. This might be important for the development of ischemic injuries. Our findings suggest that the effect of NBP might be beneficial for protection against post-ischemic neuronal damage.

Adenosine↗
PubMed1996

[Observation and analysis of postburn changes in substance P (SP) and SP peptidergic nerve fibers in the intestine of rat].

Substance P (SP) was measured by radioimmunoassay (RIA) and SP peptidergic nerve fibers were identified by Immunohistochemistry (IHC) in the intestinal wall of rats (TBSA 30% and with full-thickness burn) during 72 hours after burn. The results were as follows: (1) SP increased significantly at 1 hr and 4 hr postburn, then decreased markedly 8 hr later, and the low was maintained up to 72 hr. (2) The significant changes in SP peptidergic nerve fibers in the villi after burn were found with immunohistochemical studies including the morphology, distributive densities and SP contents of the SP peptidergic nerve fibers. The results from image analysis showed that the distributive densities of the nerve fibers elevated distinctly in 1 hr group, then dropped at 8 hr and 12 hr and finally elevated again. The results indicated that, the SP release might occur leading to SP consumption in the intestinal wall of rats after burn. It is possible that SP contributes to the postburn intestinal lesion in rats. The SP peptidergic nerve fibers in villus have a direct effect on the damage of intestinal mucosal barrier.

Animals↗
PubMed1996

[Effect of dl-3-butylphthalide on the striatum extracellular amino acid and dopamine contents in the rat during cerebral ischemia].

The effect of dl-3-butylphthalide (NBP) on the contents of amino acids and dopamine in the rat striatum during globe cerebral ischemia has been studied. By using the technique of microperfusion in the striatum of rats subjected to 4-vessel occlusion cerebral ischemia, the extracellular contents of glutamate, taurine, gamma-aminobutyric acid and dopamine were found to be significantly increased in the striatum during the 20 min of cerebral ischemia. NBP (40 mg.kg-1; i.p. 30 min before ischemia) was shown to reduce the contents of dopamine and its metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), in the striatal extracellular fluid of the rat during ischemia. The content of glycine before and after ischemia was also reduced. However, no significant effect on the contents of glutamate and some other amino acids was observed. The results suggest that NBP may improve the striatal ischemic injury.

3,4-Dihydroxyphenylacetic Acid↗