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Biomedical subjects

D Humphries

Publications and source records attributed to D Humphries.

14 recordsLinked to original sources
PubMed2004

Clinical and magnetic resonance imaging features of cricket bowler's side strain.

The clinical features of 10 cases of lateral trunk muscle injury in first class cricket pace bowlers are described. Typically the injury occurs during a single delivery, is associated with considerable pain, and prevents the bowler from continuing. The clinical picture is typical of a muscular or musculotendinous injury. The most consistent clinical tests were focal tenderness on palpation and pain with resisted side flexion towards the painful side. The magnetic resonance image in 70% of cases was consistent with an injury to the internal oblique, the external oblique, or the transversalis muscles at or near their attachments to one or more of the lowest four ribs. The injury occurs on the non-bowling arm side. Recovery can be prolonged. The injury was a recurrence in six of the 10 cases. The biomechanics of the injury are not yet understood.

Adrenal Cortex Hormones↗
PubMed2002

Magnetic bead purification of labeled DNA fragments for high-throughput capillary electrophoresis sequencing.

We have developed an automated purification method for dye-terminator-based DNA sequencing products using a magnetic bead approach. This 384-well protocol generates sequence fragments that are essentially free of template DNA, salt, and excess dye-terminator products. In comparison with traditional ethanol precipitation protocols, this method uses no centrifugation, is rapid, completely automated, and increases the phred-20 read length by an average of 40 bases. To date, we have processed over 4 million samples with 94% averaging 641 phred-20 bases on the MegaBACE 1000 and 4000 and the ABI PRISM 3700 capillary instruments.

DNA↗
PubMed1996

Acute changes in cranial blood flow after cocaine hydrochloride.

Cerebral blood flow (CBF) was measured with the 133Xenon inhalation technique and forehead skin flow with laser Doppler, before and twice after 0.3 milligram/kilogram of cocaine hydrochloride and a placebo given intravenously to six cocaine abusers, during two visits, separated by a minimum of one week. After cocaine, subjects showed significant increases in intoxication and tension. Systolic blood pressure and pulse rate also increased significantly after the drug but not after the placebo. CBF, with and without correction for end-tidal CO2, showed increases in left and right hemisphere after cocaine. The CBF increase was maximal in frontal, central and parietal regions. CBF changes correlated only with changes in a rated level of intoxication. Forehead skin flow did not change after cocaine or placebo. In habitual cocaine abusers, 0.3 mg/kg of cocaine, given intravenously produced increased CBF and no changes in forehead skin flow.

Adult↗
PubMed1996

A rapid method for determination of Aroclor 1260 in cattle adipose tissue.

A simple analytical procedure for the determination of Aroclor 1260 in cattle adipose tissue is described. The polychlorinated biphenyls residues are extracted from the tissue using a soxhlet extractor and the extracts are cleaned up using a florisil SEP-PAK cartridge. The residues are detected using a gas chromatograph equipped with an electron capture detector. The effect of extraction time of the Aroclor 1260 residues from the tissue has been investigated and a period of four hours is found to give satisfactory percent recoveries. Greater than 85 percent recoveries were obtained from adipose tissue spiked with Aroclor 1260. The method can be used to detect Aroclor 1260 residue levels as low as 0.10 parts per million. The method was used to analyze thirty-one cattle adipose tissue samples out of which twenty-six samples were taken from cattle suspected of exposure to a pasture containing electrical transformers and capacitors containing Aroclor 1260. Five control samples were collected from cattle with no known exposure. All twenty-six samples were found to contain non-detectable Aroclor 1260 residues.

Adipose Tissue↗
PubMed1994

Increased CD4+ T cell-dependent anti-erythrocyte antibody levels following the onset of parasite egg production in Schistosoma mansoni infected mice.

Anaemia has been reported to be a symptom of schistosomiasis mansoni. In other chronic infectious diseases, anti-red blood cell (RBC) antibodies have been suggested or shown to play a role in anaemia by participating in either complement or macrophage-dependent RBC elimination. To examine whether such a situation could be contributing to the anaemia of schistosomiasis, we examined RBC taken from infected mice for surface-bound antibodies. Our data show that prior to the onset of egg production infected mice have plasma haemoglobin levels that are indistinguishable from age matched controls (AMC). However, consistent with previous reports, following the initiation of egg laying, infected mice have significantly lower haemoglobin levels than AMC. Surface-bound IgM, IgG1 and IgG3 on RBC from infected mice increased markedly after egg laying began. Levels of RBC-associated IgG2b were similar on RBC from infected and normal mice. Antibody production against RBC was Th cell-dependent since it did not occur in mice depleted of CD4+ cells. Antibodies eluted from RBC of infected mice bound to isolated membranes of RBC from AMC and to a soluble extract of schistosome eggs. Furthermore, antibodies in serum from mice carrying patent infections bound to the membranes of RBC from normal mice. Taken together, these data suggest that schistosome eggs induce an antibody response which may cross react with a RBC surface antigen.

Animals↗
PubMed1989

Prosthetic usage following major lower extremity amputation.

There were 157 patients following major lower extremity amputation who were evaluated to determine functional prosthetic ambulation. Twenty-eight patients were evaluated in the amputee clinic and found not to be candidates for prosthetic fitting. Forty-one patients were fit with a prosthesis but did not become functional prosthetic ambulators. Eighty-eight patients became functional prosthetic ambulators. Of all above-the-knee amputees, 46% became functional prosthetic ambulators. Only 19% of bilateral lower extremity amputees became functional prosthetic ambulators. Of all below-the-knee amputees, 66% became functional ambulators. The presence of coronary artery disease decreased the ambulatory potential in above-the-knee or bilateral amputees but not in below-the-knee amputees. A screening method for elderly, dysvascular amputees should be instituted prior to prosthetic fitting.

Aged↗
PubMed1980

Effects of a bone resorptive factor from human cancer ascites fluid on rat bone cell calcium and cyclic AMP.

The effects of a bone resorptive protein isolated from human cancer ascites fluid on bone cell calcium and cyclic AMP were studied with fetal rat cells. The osteoclast-activating factor increased bone cell calcium uptake at 37 degrees C and 4 degrees C with no direct effects on calcium efflux. Concentrations of the resorptive factor that increased in vitro bone resorption and cell calcium uptake had no effect on cyclic AMP. The effects of the protein on calcium uptake were not specific for bone cells, and large increases were also observed in isolated fetal rat skin cells. These studies suggest that increases in the permeability of the cell membrane to calcium are involved in the mechanism of action of the ascites fluid resorptive protein.

Animals↗