Biomedical subjects

D I Kurtböke

Publications and source records attributed to D I Kurtböke.

5 recordsLinked to original sources

PubMed2002

Fighting foam with phages?

Seventeen (17) phages infective for the mycolata were isolated from six samples of activated sludge using 21 prospective hosts from the genera Dietzia, Gordonia, Nocardia, Rhodococcus, Tsukamurella and Mycobacterium. Their morphology indicated that they were all members of the viral family Siphoviridae, but they varied in the size of the icosahedral head and length of non-contractile tail, suggesting they were different. This was confirmed by host-range studies with 47 strains of mycolata, which showed that each phage had a unique host-range, and this was polyvalent in the majority (15/17) of cases, with 12 phages infective for hosts representing two or three of the genera Gordonia, Nocardia and Rhodococcus. The potential for use of these phages in the control of foaming and other applications is discussed.

Actinobacteria↗

PubMed1995

Isolation of Dermatophilus congolensis phage from the 'lumpy wool' of sheep in Western Australia.

A lytic phage with species-specific activity was isolated from wool samples infected with the actinomycete Dermatophilus congolensis, the agent of 'lumpy wool', collected from properties in Western Australia. The physiochemical properties, plaque morphology, host range and particle morphology of the phage isolated were characterized. The isolated phage reduced the cell numbers of Dermatophilus congolensis on infected wool samples in vitro. It may therefore have potential as a biocontrol agent of dermatophilosis.

Actinomycetales↗

PubMed1993

Detection and identification of novel actinomycetes.

The actinomycetes are well known as a group of filamentous, Gram-positive bacteria that produce many useful secondary metabolites, including antibiotics and enzymes. Although they have been intensively studied for both theoretical and practical objectives, there is much scope for developing our basic knowledge of the means of detection and isolation of these microbes. This session concentrated on new methods for the detection and identification of novel actinomycetes from a range of environments. Approaches to the detection of actinomycetes ranged from investigations of neglected habitats and extreme environments (e.g. alkaline soils and oil drills) to the analysis of DNA extracted from the environment and use of specific phages. The continuing problems of the identification of actinomycete isolates were also considered. Topics discussed included use of phage typing, DNA probes, and correlation between phenetic and genotypic species of Streptomyces.

Actinomycetales↗

PubMed1993

Use of bacteriophage for the selective isolation of thermophilic actinomycetes from composted eucalyptus bark.

A method was developed to reduce the numbers of thermophilic bacteria on isolation plates, which in turn facilitated the detection and isolation of thermophilic actinomycetes. The method involves exposing the test material to bacteriophage suspensions prior to inoculation on isolation plates. This method was applied to composted eucalyptus bark samples, which were then inoculated on R8 and 1/2 TSA + 0.2% casein hydrolysate agar plates. The phage susceptibility of thermophilic bacteria provided a selective means of reducing their numbers on isolation plates and hence increased the numbers of Thermomonospora, Saccharopolyspora rectivirgula, and thermophilic Streptomyces spp. on these media in comparison with the numbers recorded from control plates.

Actinomycetaceae↗

PubMed1992

Use of polyvalent phage for reduction of streptomycetes on soil dilution plates.

An isolation method was developed in which prior to inoculation soil suspensions were exposed to suspensions of polyvalent phage isolated to Streptomyces spp. The phage susceptibility of streptomycetes provided a selective means of reducing streptomycetes on isolation plates subsequent to inoculation, and this reduction was persistent after long incubation periods. The efficiency and applicability of the method developed were checked with different samples from a range of sources. The increased chances of development of other genera after the reduction of streptomycetes on soil dilution plates were assessed.

Actinomycetales↗